Bacilysin within the Bacillus subtilis group: gene prevalence versus antagonistic activity against Gram-negative foodborne pathogens

Nannan, Catherine; Vu, Huong Quynh; Gillis, Annika; Caulier, Simon; Nguyen, Thuy Thanh Thi; Mahillon, Jacques

doi:10.1016/j.jbiotec.2020.12.017

Cited by 36 publications

(22 citation statements)

References 43 publications

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“…Two antibiotic-encoding genes were also detected in the BS50 genome, including bacilysin and bacillaene. Bacilysin is a non-ribosomally synthesized dipeptide antibiotic that inhibits Gram-negative foodborne pathogens [ 107 , 108 , 109 ]. Bacillaene is a polyene antibiotic that can accelerate biofilm formation and has activity against a broad spectrum of bacteria, including S. aureus and E. coli [ 110 , 111 , 112 , 113 ].…”

Section: Discussionmentioning

confidence: 99%

Preclinical Safety Assessment of Bacillus subtilis BS50 for Probiotic and Food Applications

Brutscher¹,

Borgmeier

Garvey³

et al. 2022

Microorganisms

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Despite the commercial rise of probiotics containing Bacillaceae spp., it remains important to assess the safety of each strain before clinical testing. Herein, we performed preclinical analyses to address the safety of Bacillus subtilis BS50. Using in silico analyses, we screened the 4.15 Mbp BS50 genome for genes encoding known Bacillus toxins, secondary metabolites, virulence factors, and antibiotic resistance. We also assessed the effects of BS50 lysates on the viability and permeability of cultured human intestinal epithelial cells (Caco-2). We found that the BS50 genome does not encode any known Bacillus toxins. The BS50 genome contains several gene clusters involved in the biosynthesis of secondary metabolites, but many of these antimicrobial metabolites (e.g., fengycin) are common to Bacillus spp. and may even confer health benefits related to gut microbiota health. BS50 was susceptible to seven of eight commonly prescribed antibiotics, and no antibiotic resistance genes were flanked by the complete mobile genetic elements that could enable a horizontal transfer. In cell culture, BS50 cell lysates did not diminish either Caco-2 viability or monolayer permeability. Altogether, BS50 exhibits a robust preclinical safety profile commensurate with commercial probiotic strains and likely poses no significant health risk to humans.

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Section: Discussionmentioning

confidence: 99%

Preclinical Safety Assessment of Bacillus subtilis BS50 for Probiotic and Food Applications

Brutscher¹,

Borgmeier

Garvey³

et al. 2022

Microorganisms

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“…Bacillibactin was a specific transport system enabling Bacillus cells to accumulate and take up limited iron ions from their natural environment (Chen et al, 2009). Bacilysin participated in the antagonistic activity of B. velezensis against Gram-negative foodborne pathogens (Nannan et al, 2021). As a potent inhibitor of filamentous fungi, fengycin owned a strong antifungal ability against V. dahliae and B. cinerea (Li Q.R.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Ability and Mechanism of a Broad-Spectrum Antifungal Strain Bacillus safensis sp. QN1NO-4 Against Strawberry Anthracnose Caused by Colletotrichum fragariae

Zhang

et al. 2021

Front. Microbiol.

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Strawberry is a very popular fruit with a special taste, color, and nutritional value. Anthracnose caused by Colletotrichum fragariae severely limits fruit shelf life during post-harvest storage. Use of traditional chemical fungicides leads to serious environment pollution and threatens food safety. Biocontrol is considered as a promising strategy to manage the post-harvest fruit diseases. Here, strain QN1NO-4 isolated from noni (Morinda citrifolia L.) fruit exhibited a high antifungal activity against C. fragariae. Based on its physicochemical profiles and phylogenetic tree of the 16S rRNA sequence, strain QN1NO-4 belonged to the genus Bacillus. The average nucleotide identity (ANI) calculated by comparing two standard strain genomes was below 95–96%, suggesting that the strain might be a novel species of the genus Bacillus and named as Bacillus safensis sp. QN1NO-4. Its extract effectively reduced the incidence of strawberry anthracnose of harvested fruit. Fruit weight and TSS contents were also maintained significantly. The antifungal mechanism assays indicated that the extract of the test antagonist inhibited mycelial growth and spore germination of C. fragariae in vitro. Cells of strain QN1NO-4 demonstrated the cytoplasmic heterogeneity, disappeared organelles, and ruptured ultrastructure. Notably, the strain extract also had a broad-spectrum antifungal activity. Compared with the whole genome of strain QN1NO-4, several functional gene clusters involved in the biosynthesis of active secondary metabolites were observed. Fifteen compounds were identified by gas chromatography–mass spectrometry (GC-MS). Hence, the fruit endophyte B. safensis sp. QN1NO-4 is a potential bio-agent identified for the management of post-harvest disease of strawberry fruit.

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“…Antagonistic effect of B. subtilis against clinical human isolates of E. coli due to its fibrinolytic activity (Jeong et al, 2015;Irkitova et al 2018) were also reported. Recently Nannan et al (2021) reported the bacilycin production ability of B. subtilis which is an important antimicrobial peptide that gives this species the opportunity to be evaluated as a probiotic. Antagonistic effect of B. subtilis was also evaluated against fish pathogens and Das et al ( 2014) reported a strong antagonistic effect against fish originated isolates of Pseudomonas aeruginosa, Edwardsiella tarda, Vibrio parahaemolyticus, Flavobacterium columnare and Staphylococcus aureus, but they also reported a negative antagonistic effect against Aeromonas hydrophila.…”

Section: Resultsmentioning

confidence: 99%

A mixed Bacillus gibsonii and Sphingomonas echinoides infection in cultured rainbow trout (Oncorhynchus mykiss)

Çanak¹,

Akayli²,

Ürkü³

2021

Marine and Life Sciences

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Bacilysin within the Bacillus subtilis group: gene prevalence versus antagonistic activity against Gram-negative foodborne pathogens

Cited by 36 publications

References 43 publications

Preclinical Safety Assessment of Bacillus subtilis BS50 for Probiotic and Food Applications

Preclinical Safety Assessment of Bacillus subtilis BS50 for Probiotic and Food Applications

Biocontrol Ability and Mechanism of a Broad-Spectrum Antifungal Strain Bacillus safensis sp. QN1NO-4 Against Strawberry Anthracnose Caused by Colletotrichum fragariae

A mixed Bacillus gibsonii and Sphingomonas echinoides infection in cultured rainbow trout (Oncorhynchus mykiss)

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