Bacillus velezensis supernatant mitigates tomato Fusarium wilt and affects the functional microbial structure in the rhizosphere in a concentration-dependent manner

Másmela-Mendoza, Julian Esteban; Velandia, Carlos Andrés Moreno

doi:10.1016/j.rhisph.2022.100475

Cited by 6 publications

(3 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results showed that compared with the CK treatment, the AWCD values (representing microbial metabolic activity) were significantly increased in the CF-BF treatment. These findings were in accordance with those reported by Másmela-Mendoza et al [53], where the application of the culture filtrate of B. velezensis Bs006 increased microbial metabolic activity in the wilt-diseased tomato plant rhizosphere. Root exudates, which are secreted at the soil-root interface, have been considered the most important factor affecting microbial metabolic function in rhizosphere soil [52].…”

Section: Discussionsupporting

confidence: 93%

“…The loss of microbial catabolic activity or of the diversity of carbon source use is regarded as a warning of diminishing agricultural soil health [52]. Several reports have indicated that the microbial function in carbon source utilization decreased significantly in the rhizosphere soil of diseased plants with vascular wilting symptoms [53]. Our results showed that compared with the CK treatment, the AWCD values (representing microbial metabolic activity) were significantly increased in the CF-BF treatment.…”

Section: Discussionmentioning

confidence: 54%

See 1 more Smart Citation

Co-Culture of White Rot Fungi Pleurotus ostreatus P5 and Bacillus amyloliquefaciens B2: A Strategy to Enhance Lipopeptide Production and Suppress of Fusarium Wilt of Cucumber

Xu,

Shi,

Fan

et al. 2023

JoF

View full text Add to dashboard Cite

Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC), poses a serious threat to cucumber productivity. Compared to traditional chemical pesticides, biological control strategies have attracted more attention recently owing to their effectiveness against pathogens and their environmental safety. This study investigated the effect of white rot fungi Pleurotus ostreatus P5 on the production of cyclic lipopeptides (CLPs) of Bacillus amyloliquefaciens B2 and the potential co-culture filtrate of strains B2 and P5 to control cucumber Fusarium wilt. A PCR amplification of CLP genes revealed that B. amyloliquefaciens B2 had two antibiotic biosynthesis genes, namely, ituA and srf, which are involved in iturin A and surfactin synthesis. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) revealed that CLPs derived from strain B2 contained two families, iturin A (C14, C15) and surfactin (C12–C17). The co-culture exhibited an enhanced accumulation of iturin A and surfactin compared to the monoculture of strain B2. Furthermore, the gene expressions of ituA and srf were both significantly upregulated when co-cultured with the fungus compared to monocultures. In an in vitro experiment, the co-culture filtrate and monoculture filtrate of B. amyloliquefaciens B2 inhibited mycelial growth by 48.2% and 33.2%, respectively. In a greenhouse experiment, the co-culture filtrate was superior to the monoculture filtrate in controlling cucumber Fusarium wilt disease and in the promotion of plant growth. Co-culture filtrate treatment significantly enhanced the microbial metabolic activity and decreased the abundance of FOC in the rhizosphere soil. These results show that the co-culture of P. ostreatus P5 and B. amyloliquefaciens B2 has great potential in cucumber Fusarium wilt disease prevention by enhancing the production of bacterial CLPs.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 54%

Co-Culture of White Rot Fungi Pleurotus ostreatus P5 and Bacillus amyloliquefaciens B2: A Strategy to Enhance Lipopeptide Production and Suppress of Fusarium Wilt of Cucumber

Xu,

Shi,

Fan

et al. 2023

JoF

View full text Add to dashboard Cite

show abstract

“…Meanwhile, tomato production in Indonesia remains relatively low at 6.3 tons ha -1 when compared to Taiwan, Saudi Arabia and India, which produce 21, 13.4 and 9 tons ha -1 , respectively (Alwi et al, 2022). Several pathogens significantly impact tomato plant production, including Fusarium oxysporum, which causes Fusarium wilt disease leading to suboptimal results (Rojas et al, 2020;Abdel-Aziz et al, 2021;Khalil et al, 2021;Bahroun et al, 2021;Másmela-Mendoza and Moreno-Velandia, 2022). Furthermore, F. oxysporum can cause 70% to 90% damage in infected tomato plants and up to 50% production losses (Jamil et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Nurlila¹,

Fua

Abidin³

et al. 2022

Caraka Tani J. Sustain. Agric.

View full text Add to dashboard Cite

Fusarium wilt is a plant disease caused by Fusarium oxysporum that generates significant economic loss to crops. A method to sustainably control F. oxysporum is utilizing biological agents, such as endophytic bacteria. Therefore, this study aimed to isolate endophytic bacteria from tomato plant tissue, which could inhibit the pathogen of wilt disease (F. oxysporum). Endophytic bacteria were isolated from local tomato plants in Muna Regency, Indonesia. Morphological characteristics such as size, shape, color and height of bacterial colonies were then determined by Gram staining using potassium hydroxide (KOH). Endophytic bacterial isolates were evaluated for their ability to inhibit F. oxysporum through inhibition and hydrogen cyanide (HCN) production tests. Subsequently, analysis of variance was used to determine whether endophytic bacteria inhibited F. oxysporum growth, and if there was a significant effect, Duncan's test was conducted at 95% significance. HCN production was observed through qualitative methods. The results showed that four endophytic bacteria isolates, namely LBR I A03, SWR II B04, SDM II B05 and SWR I A02 inhibited the growth of F. oxysporum by more than 50%. It also revealed that four endophytic bacterial isolates were strong HCN producers and two were weak producers. Therefore, isolates showing antifungal activity in this study can be used as biopesticide agents to induce plant resistance to F. oxysporum.

show abstract

Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

Moreno-Velandia,

Izquierdo-García,

García-Arias

et al. 2024

Eur J Plant Pathol

View full text Add to dashboard Cite

Clubroot disease caused by Plasmodiophora brassicae is the most devastating disease in Brassicaceae plants. Control of clubroot is limited because of the survival of resting spores of the pathogen in the soil for years. Crop rotation, liming of the soil, fungicides and resistant cultivars have not been totally efficient in reducing the damages of the disease or pathogen spread. Although biocontrol with microorganisms has shown potential against clubroot, the variable results when using individual strains have made implementation difficult. Thus, the aim of this work was to determine whether a consortium of selected beneficial microorganisms is more efficient in controlling clubroot in broccoli than single strains. Single strains of bacteria from the Lysinibacillus, Bacillus and Pseudomonas genera (5 × 107 cells ml−1) and Trichoderma spp. (1 × 106 conidia ml−1) were evaluated in an initial screening. Four strains showing the best performance on plant growth and reduction of clubroot were evaluated as consortia in a further experiment. Treatments were applied in nursery (4 ml plant−1), and to the soil one week before transplant and the day of transplant (60 ml plant−1 each time). Plant shoot and root dry weight and the incidence and severity of clubroot were recorded. Supernatant from liquid cultures of Pseudomonas migulae Pf014 and Lysinibacillus xylanilyticus Br042, and washed cells of Bacillus velezensis Bs006 and B. pumilus Br019 promoted shoot growth of the plants. Single applications of Trichoderma asperellum Th034, Bs006, Br042 and Pseudomonas fluorescens Ps006 showed biocontrol potential against clubroot. However, the consortia built of Bs006, Br042 and Ps006 showed the highest reduction of clubroot incidence (78%) and severity (74%). A synergistic interaction by this consortium was found compared with the efficacy of single strains. To our knowledge, this work reports for the first time a consortium based on three rhizobacteria with high potential to control clubroot disease.

show abstract

Bacillus velezensis supernatant mitigates tomato Fusarium wilt and affects the functional microbial structure in the rhizosphere in a concentration-dependent manner

Cited by 6 publications

References 49 publications

Co-Culture of White Rot Fungi Pleurotus ostreatus P5 and Bacillus amyloliquefaciens B2: A Strategy to Enhance Lipopeptide Production and Suppress of Fusarium Wilt of Cucumber

Co-Culture of White Rot Fungi Pleurotus ostreatus P5 and Bacillus amyloliquefaciens B2: A Strategy to Enhance Lipopeptide Production and Suppress of Fusarium Wilt of Cucumber

Effectiveness of Endophytic Bacteria from Local Tomato Plants Against Wilt Disease Caused by Fusarium oxysporum

Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

Contact Info

Product

Resources

About