Bacillus velezensis Identification and Recombinant Expression, Purification, and Characterization of Its Alpha-Amylase

Zhang, Xiaodong; Li, Caixia; Chen, Xuantong; Chio, Chonlong; Shrestha, Sarita; Khatiwada, Janak Raj

doi:10.3390/fermentation7040227

Cited by 7 publications

(3 citation statements)

References 50 publications

(97 reference statements)

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“…The bacterial lysates were checked using a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and a protein band of the expected molecular weight in the expressed proteins of the bacteria confirmed the fusion protein expression. The experimental process is similar to that described by Zhang et al [ 45 ].…”

Section: Methodsmentioning

confidence: 78%

Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A

Yang

et al. 2023

Genes

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Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family 1 (GH1) using bioinformatic methods based on the wild jujube genome. The conserved domains and motifs of the 35 putative β-glucosidases, along with the genome locations and exon–intron structures of 35 β-glucosidase genes were revealed. The potential functions of the putative proteins encoded by the 35 β-glucosidase genes are suggested based on their phylogenetic relationships with Arabidopsis homologs. Two wild jujube β-glucosidase genes were heterologously expressed in Escherichia coli, and the recombinant proteins were able to convert jujuboside A (JuA) into jujuboside B (JuB). Since it has been previously reported that JuA catabolites, including JuB and other rare jujubosides, may play crucial roles in the jujuboside’s pharmacological activity, it is suggested that these two proteins can be used to enhance the utilization potential of jujubosides. This study provides new insight into the metabolism of jujubosides in wild jujube. Furthermore, the characterization of β-glucosidase genes is expected to facilitate investigations involving the cultivation and breeding of wild jujube.

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Section: Methodsmentioning

confidence: 78%

Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A

Yang

et al. 2023

Genes

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“…In this work, we found that most of the Bacillaceae strains displayed the amylase-producing ability in the artifcial ponds and earthen ponds, particularly the genera of Bacillus and Halobacillus. Numerous Bacillus species were identifed as the producer of amylases, such as B. licheniformis [41], B. amyloliquefaciens [42], B. subtilis [43], B. cereus [44], B. paramycoides [45], B. koreensis [46], and B. velezensis [47]. At the same time, there were relatively few reports on Halobacillus species with the capacity of producing amylases, such as Halobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Contribution of the Types of Tropical Coastal Aquaculture Ponds to the Differential Distribution of the Family Bacillaceae Bacterial Community

Ren

Feng

Wang

et al. 2023

Aquaculture Research

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The family Bacillaceae bacteria, particularly Bacillus communities, are useful and attractive probiotics with desirable features to fulfill the vast demand for aquaculture. In Hainan Island, the coastal aquaculture ponds hold about 1% of the total area in China, whereas the knowledge of Bacillaceae bacterial communities was limited. Herein, the Bacillaceae bacterial communities in the coastal aquaculture ponds of the artificial ponds (lined with high-density polyethylene plastic sheets) and earthen ponds were investigated by the culturable method. The characteristics of culturable Bacillaceae communities in these two types of coastal aquaculture ponds showed a massive difference. All the culturable Bacillaceae bacteria were identified as Bacillus in the artificial ponds, whereas the earthen ponds were dominated by Bacillus followed by Halobacillus. It is worth noting that the artificial ponds with a low Bacillaceae diversity displayed a high Bacillaceae concentration with a better water quality, whereas the earthen ponds with a higher Bacillaceae diversity and concentration displayed a higher risk of Vibrio infection, indicating that the Bacillaceae concentration than its diversity made a greater contribution to water purification and Vibrio control. This work besides providing a promising strategy for healthy coastal aquaculture by balancing the relationship between Bacillaceae concentration and its diversity also will lay a foundation for the development of probiotic Bacillaceae agents for wastewater purification and disease control.

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“…The α-amylase gene from B. velezensis WangLB was cloned, expressed, purified, and characterized. The purified α-amylase maintained high activity over a wide range of temperatures [30] . A novel fibrinolytic serine metalloprotease gene and a galactosidase gene from B. velezensis SW5 were heterologously expressed in E. coli [31][32] .…”

Section: Bioinformatics Study Of B Velezensismentioning

confidence: 97%

Research advance of Bacillus velezensis: bioinformatics, characteristics, and applications

Su,

Shen,

et al. 2024

Food Science and Human Wellness

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Bacillus velezensis is a Gram-positive and spore-forming bacterium. It has potent antimicrobial properties that can be used to promote plant growth and as a pesticide by inhibiting pathogens. B. velezensis has the capability to generate a diverse range of enzymes that have potential applications in various fields, such as enzyme production, fermented food, degradation of pollutants, and bioenergy. In addition, B. velezensis is a promising probiotic. It possesses high bile-salt tolerance characteristics and has a high success rate of colonization in the intestinal mucosa. Besides, the strain can also regulate gut microbiota constitute by increasing the number of beneficial microorganisms and decreasing the number of pathogens. Furthermore, based on its special properties, including high-yield protease production and high salt-tolerance, B. velezensis shows potential for use in marine protein fermentation, opening up new avenues for the development of novel food products and bioactive peptides. In addition, B. velezensis can shorten the fermentation time as well as improve the nutritional value and fl avor of fermented food. The safety of B. velezensis for food production was evaluated. This review provides valuable insights into the potential uses and benefi ts of B. velezensis, particularly in the context of fermented foods.

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Bacillus velezensis Identification and Recombinant Expression, Purification, and Characterization of Its Alpha-Amylase

Cited by 7 publications

References 50 publications

Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A

Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A

Contribution of the Types of Tropical Coastal Aquaculture Ponds to the Differential Distribution of the Family Bacillaceae Bacterial Community

Research advance of Bacillus velezensis: bioinformatics, characteristics, and applications

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