Bacillus velezensis, a potential and efficient biocontrol agent in control of pepper gray mold caused by Botrytis cinerea

Jiang, Chunhao; Liao, Mengjie; Wang, Hongkai; Zheng, Mingzi; Jian-jun, XU; Guo, Jianhua

doi:10.1016/j.biocontrol.2018.07.017

Cited by 141 publications

(69 citation statements)

References 41 publications

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“…For example, transmission electron microscopy observation of fumigated and untreated B. cinerea showed excessive vesication or thickened cell walls in exposed conidia and increased strong retraction of plasma membrane in exposed hyphae (Li et al, 2012). The VOCs of B. velezensis 5YN8 can suppress the mycelium growth and conidia formation of B. cinerea BC1301 (Jiang et al, 2018). In this study, the insights into the mechanisms of B. subtilis ZD01 volatiles against A. solani showed similar biocontrol strategies.…”

Section: Discussionmentioning

confidence: 53%

“…In several recent studies, VOCs produced by Bacillus species have been identified to inhibit the airborne pathogens, mainly as Botrytis cinerea, the causal agent of tomato gray mold. Two Bacillus velezensis strains 5YN8 and DSN012 could suppress the growth and spore formation of B. cinerea by releasing numbers of VOCs (Jiang et al, 2018). The VOCs of B. velezensis ZSY-1 strain exhibit significant antifungal activity against B. cinerea (Gao et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Dai

Yang

et al. 2020

Front. Microbiol.

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Antifungal activities of plant-beneficial Bacillus have been widely studied in recent years. Numerous studies have studied the antifungal mechanisms of soluble nonvolatile bioactive compounds such as lipopeptides and proteins produced by Bacillus against soil-borne diseases. However, the antagonistic mechanisms of volatile organic compounds (VOCs) from Bacillus against airborne phytopathogens are still largely unknown, and the function of Alternaria solani pathogenic genes has not been well identified. Here, we first isolated a Bacillus strain with strong antifungal activity and finally identified it as B. subtilis ZD01. Then, the antagonistic mechanisms of VOCs produced by strain ZD01, against A. solani, an airborne fungal pathogen that can cause early blight diseases of potato, were studied. We showed that VOCs produced by strain ZD01 can reduce the colony size and mycelial penetration and can cause serious morphological changes of A. solani. Scanning electron microscope (SEM) observation showed that VOCs released by ZD01 could cause more flaccid and gapped hyphae of A. solani. Also, we found that VOCs produced by ZD01 can inhibit the conidia germination and reduce the lesion areas and number of A. solani in vivo significantly. Meanwhile, based on gas chromatography/mass spectrometry (GC/MS) analysis, 29 volatile compounds produced by strain ZD01 were identified. Out of 29 identified VOCs, 9 VOCs showed complete growth inhibition activities against A. solani. Moreover, we identified two virulence-associated genes (slt2 and sod) in A. solani. slt2 is a key gene that regulates the mycelial growth, penetration, sporulation, and virulence in vivo in A. solani. In addition, sod plays a significant role in the SOD synthetic pathway in A. solani. Results from qRT-PCR showed that the transcriptional expression of these two genes was down-regulated after being treated by VOCs produced by ZD01. These results are useful for a better understanding of the biocontrol mechanism of Bacillus and offer a potential method for potato early blight disease control.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Dai

Yang

et al. 2020

Front. Microbiol.

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“…could enhance the activity of defense enzymes in rice [22], tomato [23], and peach [24], etc. There are a few reports showing that bacterial VOC can influence the activity of some defense enzymes of fruit and antioxidant enzymes of plant pathogens significantly [25][26][27]. Although the mechanisms by which microbial antagonists control postharvest diseases have not been clearly elucidated, induced disease resistance has been inferred to be one of the major modes of their actions [4].…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Volatile Organic Compounds Produced by Bacillus methylotrophicus and Bacillus thuringiensis against Five Common Spoilage Fungi on Loquats

Zhu

et al. 2020

Molecules

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Loquat fruit is one of the most perishable fruits in China, and has a very limited shelf life because of mechanical injury and microbial decay. Due to an increasing concern about human health and environmental security, antagonistic microorganisms have been a potential alternative for fungicides to control postharvest diseases. In this work, the antifungal effect of volatile organic compounds (VOCs) produced by Bacillus methylotrophicus BCN2 and Bacillus thuringiensis BCN10 against five postharvest pathogens isolated from loquat fruit, Fusarium oxysporum, Botryosphaeria sp., Trichoderma atroviride, Colletotrichum gloeosporioides, and Penicillium expansum were evaluated by in vitro and in vivo experiments. As a result, the VOCs released by BCN2 and BCN10 were able to suppress the mycelial growth of all targeted pathogens according to inhibition ratio in the double petri-dish assay as well as disease incidence and disease diameter on loquat fruits. The main volatile compounds were identified by solid-phase microextraction (SPME)-gas chromatography. These VOCs produced by the two strains played complementary roles in controlling these five molds and enabled loquat fruits to keep fresh for ten days, significantly. This research will provide a theoretic foundation and technical support for exploring the functional components of VOCs applicable in loquat fruit preservation.

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“…Experiments in vitro that assessed the antagonism of rhizobacteria against P. capsici indicated that six strains exhibited strong suppression of mycelial growth of the fungal pathogen. Such strong antifungal activity in vitro can be attributed to the production of a variety of antimicrobial compounds that cause cytolysis, disruption of the structural integrity of membranes, inhibition of mycelial growth and inhibition of spore germination (Gu et al 2017;Jiang et al 2018;Luna-Bulbarela et al 2018;Niu et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Growth-promoting and disease-suppressing effects of Paenibacillus polymyxa strain YCP16-23 on pepper (Capsicum annuum) plants

Jing

Guo

et al. 2020

Trop. plant pathol.

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Phytophthora blight is the most devastating disease for pepper (Capsicum annuum L.) production and is caused by Phytophthora capsici. Current management strategies are not effective. Our study assessed the multi-trait effects of Paenibacillus polymyxa, which suppresses Phytophthora blight and promotes plant growth in pepper plants. A total of 82 bacterial strains isolated from rhizosphere soil of pepper plants were screened for in vitro inhibition of P. capsici growth. The results revealed that only six strains showed strong antagonistic activity against P. capsici. These six strains were used for controlling Phytophthora blight in pepper by seedling assays under greenhouse conditions. Three strains showed a lower incidence of Phytophthora blight and were used in further growth-promoting and biocontrol studies under greenhouse conditions. Strain YCP16-23 showed >60.0% suppression of P. capsici and significantly promoted growth of pepper plants compared to the untreated control. Based on cultural and morphological characterization and 16S rRNA gene sequencing, strain YCP16-23 was identified as Paenibacillus polymyxa. Strain YCP16-23 demonstrated many characteristics that are beneficial for plants, such as production of α-amylase, ammonia, cellulase, indole-3-acetic acid, pectinase, siderophores, and protease, as well as solubilization of phosphate. In a twoyear field trial, strain YCP16-23 exhibited remarkable disease suppressing effect by controlling Phytophthora blight (76.24% and 38.09% in years 2017 and 2018, respectively) compared with the untreated control. Strain YCP16-23 caused disease suppression comparable to the microbial fungicides 'Cike' (72.04% and 15.41%) and Fluazinam (80.47% and 5.24%). Overall, the results suggest that strain YCP16-23 could provide protection against Phytophthora blight via direct and indirect modes of action and can be used as a biocontrol agent.

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Bacillus velezensis, a potential and efficient biocontrol agent in control of pepper gray mold caused by Botrytis cinerea

Cited by 141 publications

References 41 publications

Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Antifungal Effects of Volatiles Produced by Bacillus subtilis Against Alternaria solani in Potato

Antifungal Activity of Volatile Organic Compounds Produced by Bacillus methylotrophicus and Bacillus thuringiensis against Five Common Spoilage Fungi on Loquats

Growth-promoting and disease-suppressing effects of Paenibacillus polymyxa strain YCP16-23 on pepper (Capsicum annuum) plants

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