Bacillus velezensis 5113 Induced Metabolic and Molecular Reprogramming during Abiotic Stress Tolerance in Wheat

Abstract

Abiotic stresses are main limiting factors for agricultural production around the world. Plant growth promoting rhizobacteria (PGPR) have been shown to improve abiotic stress tolerance in several plants. However, the molecular and physiological changes connected with PGPR priming of stress management are poorly understood. The present investigation aimed to explore major metabolic and molecular changes connected with the ability of Bacillus velezensis 5113 to mediate abiotic stress tolerance in wheat. Seedlings treated with Bacillus were exposed to heat, cold/freezing or drought stress. Bacillus improved wheat survival in all stress conditions. SPAD readings showed higher chlorophyll content in 5113-treated stressed seedlings. Metabolite profiling using NMR and ESI-MS provided evidences for metabolic reprograming in 5113-treated seedlings and showed that several common stress metabolites were significantly accumulated in stressed wheat. Two-dimensional gel electrophoresis of wheat leaves resolved more than 300 proteins of which several were differentially expressed between different treatments and that cold stress had a stronger impact on the protein pattern compared to heat and drought. Peptides maps or sequences were used for database searches which identified several homologs. The present study suggests that 5113 treatment provides systemic effects that involve metabolic and regulatory functions supporting both growth and stress management.

PGPR Mediated Alterations in Root Traits: Way Toward Sustainable Crop Production

“…Treatment of wheat seedlings with Bacillus velezensis 5113 exposed to cold/freezing, heat or drought stress, improved the plant survival under stress conditions. Protein profile of wheat leaves showed differential expression of several proteins with cold stress showing strong impact on protein profile than heat and drought (Abd El-Daim et al, 2019). PGPR also help the plant under stress conditions by nutrient cycling through diazotrophy, phosphorus solubilization, and siderophore synthesis, by enhancing photosynthesis, increasing fine root production and overall root surface area and reducing the stress ethylene emission, thus contributing toward better plant performance (Casanovas et al, 2002;Timmusk et al, 2014;Gagné-Bourque et al, 2016).…”

Plant Responses to Abiotic Stresses and Rhizobacterial Biostimulants: Metabolomics and Epigenetics Perspectives

“…The rhizosphere chemistry and the development of plant-rhizomicrobiome interactions are still poorly characterised, however, emerging studies have reported that various PGPR species can pre-condition the plants for augmented defence responses against abiotic stresses [ 160 , 161 , 162 , 163 , 164 ]. The molecular mechanisms underlying the rhizobacteria-related defence priming show that this induced state suggests a reprogramming in the cellular metabolism and regulatory machinery of the plants.…”

PGPR in Agriculture: A Sustainable Approach to Increasing Climate Change Resilience

“…Implementing a sustainable strategy to improve plant resistance against such environmental limitations is of great importance to secure and optimize global food production. One of the eco-friendly approaches is the application of PGPR and/or their byproducts (Mayak et al, 2004;Bano and Fatima, 2009;Piccoli and Bottini, 2013;Zafar-Ul-Hye et al, 2014;Qin et al, 2016;Abd El-Daim et al, 2019;Ipek et al, 2019), which can sustainably assist the plants to withstand the extreme environmental conditions (Table 1).…”