Bacillus Thuringiensis Var. Israelensis Como Alternativa Para O Controle Populacional De Aedes Aegypti (Linnaeus, 1762) (Diptera: Culicidae)

Abstract

Dengue, chikungunya and zika are among the major problems to public health problem in the world. In the absence of vaccine, the only known alternative to prevention is to control the mosquito. Among the methods of control, using biological strains of Bacillus thuringiensis israelensis (Bti) has stood out for its efficiency and security in the application. This study aimed to evaluate the efficiency of Bti to control the population of the Aedes aegypti mosquito in junkyards and recycling centers of Chapecó, Santa Catarina. Seven applications of Bti were conducted once every 21 days, three junkyards and three recycling centers, from August to December 2014. Was used wettable powder, soluble in water and applied at a concentration of 800 grams per hectare according to the manufacturer's technical specifications. The monthly number of larvae and of eggs and larvae of A. aegypti were recorded and compared with the results obtained in junkyards and recycling control. It was observed a reduction of 89.6% in the number of eggs and mosquito larvae in establishments that received treatment with Bti, compared to the establishments control. The infestation occurred two months earlier in establishments that did not receive treatment. The Bti was efficient in the mosquito A. aegypti population control in junkyards and recycling centers in tested conditions. It is presented as an alternative for the prevention of dengue, chikungunya and zika virus.

Efficacy Bacillus thuringiensis var. israelensis serotype H-14 (Bti H-14) for control Aedes spp. density in Denpasar, Bali

“…A study in Brazil using Bti in containers in recycling and junkyard showed an 89.6% reduction in larvae. 27…”

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

“…Bt is a Gram-positive bacterium known for its toxicity and specificity towards insect hosts due to its ability to produce and release crystal proteins (Cry and Cyt) during the sporulation stage [3]. Bacillus thuringiensis serovar israelensis (Bti) is one of the subspecies' most effective larvicides for mosquito control, being recommended by the World Health Organization (WHO) [4,5]. Bti produces Cry and Cyt crystals (Cry4Aa, Cry4Ba, Cry10Aa, Cry11Aa, Cyt1Aa, and Cyt2Ba) that exhibit toxicity against mosquito species from the genus Aedes, Anopheles, and Culex, and for the black fly (Simuliidae) [4,[6][7][8].…”

“…Description based on Blastx from NCBI 4. The number of peptide sequences that are unique to the protein 5. The percentage of the protein sequence covered by identified peptides 6.…”

Biopolymer Microencapsulation of Bacillus thuringiensis israelensis Tested against Two Populations of Aedes aegypti1 Larvae

“…Significant reduction was observed in the number of breeding sites of A. aegypti in places treated with Bti compared with those that were not (checks) because these had five times more breeding sites than did treated ones. In general, numbers of mosquito eggs and larvae were 89% less with the applied product (Lutinski et al 2017).…”