Bacillus thuringiensis growth and toxicity

Avignone–Rossa, Claudio; Mignone, Carlos Fernando

doi:10.1007/bf02907471

Cited by 26 publications

(14 citation statements)

References 139 publications

(164 reference statements)

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“…Oxygen is necessary for all aerobic fermentation. Maintaining the appropriate concentration of dissolved oxygen has been pointed out as an important factor in the fermentation of B. thuringiensis (Avignone- Rossa & Mignone 1995;Morris et al 1996;Flores et al 1997) Although there is a considerable amount of literature available in relation to the effects of oxygen on the biomass concentration (Liu et al 1994) and toxin synthesis (Foda et al 1985;Dulmage et al 1990;Maldonado-Blanco et al 2003) the research was invariably carried out under constant aeration conditions, all along the culture, in flasks or laboratory fermenters. However the oxygen demands of culture are not the same all along the culture and depend strictly on the dominant physiological state of the microbial population in the culture.…”

Section: Introductionmentioning

confidence: 98%

The effect of oxygen on the sporulation, δ-endotoxin synthesis and toxicity of Bacillus thuringiensis H14

Sarrafzadeh

Navarro

2005

World J Microbiol Biotechnol

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The sporulation and toxicity of Bacillus thuringiensis H14 were studied as a function of aeration. The fed-batch cultures carried out in the similar aeration conditions were followed in four different oxygen transfer rates containing 0, 20, 100 and 250 mmol/l/h. The percentage of total cells which had formed refractile spores in these four oxygen transfer rates were 100, 93, 84 and 48%, respectively. The highest rate of sporulation was observed in the absence of oxygen and the mature spores were the only population present under this condition at the end of culture. Sporulation in a large portion of cells failed under saturated oxygenation and either mature spores or vegetative cells were present at the end of culture. In the intermediate conditions, cells in different physiological states could be observed at the end of culture. It was found that the optimal conditions for spore yield and for d-endotoxin yield were not the same, even though sporulation and d-endotoxin formation proceed simultaneously during the fermentation process. The 130-kDa d-endotoxin seemed to be more sensitive to aeration conditions. The higher toxicity against Culex pipiens was obtained under the saturated condition.

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Section: Introductionmentioning

confidence: 98%

The effect of oxygen on the sporulation, δ-endotoxin synthesis and toxicity of Bacillus thuringiensis H14

Sarrafzadeh

Navarro

2005

World J Microbiol Biotechnol

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“…On the order of 13,000 tonnes of Bt is used worldwide annually to control pests on a wide range of food and fiber crops, in commercial forestry, and in ornamental crops, as well as in environmentally benign programs for control of diseases spread by mosquito and black fly vectors (2,3). Knowledge on both fundamental aspects of the biology, biochemistry and genetics of this microorganism and applied considerations related to production of Bt bioinsecticides has been reviewed within recent years (4,5).…”

Section: Introductionmentioning

confidence: 99%

Specific Oxygen Uptake Rate Variations during Batch Fermentation of Bacillus thuringiensis Subspecies kurstaki HD‐1

Rowe

Margaritis

Wei

2003

Biotechnology Progress

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The specific oxygen uptake rate (q(O)2, respiration rate) of Bacillus thuringiensis subsp. kurstaki HD-1 was very high at inoculation and was found to decrease essentially monotonically throughout both vegetative growth phase and transition phase under different batch culture conditions. Average q(O)2 values decreased from 8-10 mmol/g h at 1 h after inoculation to less than 2 mmol/g h by the time growth ended. The results are shown to be consistent with the few previous reports on q(O)2 in B. thuringiensis in the literature but also novel in that this pattern of monotonic decline has not been described previously. Both pH control and EDTA in low concentration shortened the vegetative growth phase and reduced the 10 h biomass concentration. Using plots of q(O)2 versus specific growth rate, mu, biomass yield based on the oxygen used for growth, was calculated for transition phase to be 0.041-0.047 g/mmol, consistent with literature values. The same plot also showed that the presence of EDTA resulted in an atypical q(O)2-mu trajectory and apparently much higher biomass yield from the oxygen consumed.

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“…not classified as genetically modified and therefore more likely to pass public perception for field testing). The parent strain of B. thuringiensis cry À HD-1 was approved for commercial production and field release and has a significant amount of toxicology data (Anon 1986, 1999, Beegle and Yamamoto 1992Agaisse and Lereclus 1995;Avignone-Rossa and Mignone 1995;Bishop and Robinson 2014). Spore decontamination data also support selection of acrystalliferous B. thuringiensis spores as surrogates for B. anthracis (Buhr et al 2012a(Buhr et al , 2013(Buhr et al , 2015aProkop et al 2014).…”

Section: Acrystalliferous Bacillus Thuringiensis Sporesmentioning

confidence: 99%

Hot, humid air decontamination of a C-130 aircraft contaminated with spores of two acrystalliferous Bacillus thuringiensis strains, surrogates for Bacillus anthracis

et al. 2016

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Bacillus thuringiensis growth and toxicity

Cited by 26 publications

References 139 publications

The effect of oxygen on the sporulation, δ-endotoxin synthesis and toxicity of Bacillus thuringiensis H14

The effect of oxygen on the sporulation, δ-endotoxin synthesis and toxicity of Bacillus thuringiensis H14

Specific Oxygen Uptake Rate Variations during Batch Fermentation of Bacillus thuringiensis Subspecies kurstaki HD‐1

Hot, humid air decontamination of a C-130 aircraft contaminated with spores of two acrystalliferous Bacillus thuringiensis strains, surrogates for Bacillus anthracis

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