Bacillus thuringiensis as Potential Biocontrol Agent for Sustainable Agriculture

Pohare, Manoj; Wagh, Sopan Ganpatrao; Udayasuriyan, V.

doi:10.1007/978-981-15-6949-4_18

Cited by 9 publications

(4 citation statements)

References 193 publications

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“…The Bt TOD651 genome also harbors different virulence factor genes, such as hemolysins, enterotoxins, proteases, and phospholipases. These virulence factor genes, conserved in the Bacillus cereus group, are responsible for the colonization and adaptation of Bt in insect hosts [51][52][53].…”

Section: Discussionmentioning

confidence: 99%

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

et al. 2023

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The combination of genomic and proteomic analyses is a useful tool for the study of novel Bacillus thuringiensis (Bt) strains, as these approaches allow the accurate identification of pesticidal proteins and virulence factors produced. Here, we isolated and evaluated the potential of a novel Neotropical Bt strain (TOD651) for controlling larvae of Aedes aegypti and Culex quinquefasciatus mosquitoes. Aiming for the full comprehension of the TOD651 larvicidal potential, we further evaluated the whole TOD651 genome and conducted the proteomic analysis of the TOD651 spore–crystal mixtures. Our results showed that Bt TOD651 similarly killed both A. aegypti (0.011 µg/mL) and C. quinquefasciatus (0.023 µg/mL) larvae, exhibiting similar potency to the commercial Bt strain. The genome sequence revealed that Bt TOD651 harbors cry11Aa3, cry10Aa4, cry4Aa4, cry4Ba5, cyt1Aa5, cyt1Ca1, cyt2Ba13, mpp60Aa3, and mpp60Ba3. The proteomic analysis revealed no expression of Mpp60Aa3, while all the other pesticidal proteins were expressed (Cry4Ba5 was more abundant than Cyt1Aa5). The expression of the Mppe showed the major proportions between proteases. The virulent factor neutral protease B and spore coat proteins were also expressed. The expression of relevant pesticidal proteins (e.g., Cry, Cyt, Mpp, and other pathogenic factors), whose actions can occur in a synergic relation, indicates that the biocontrol using Bt TOD651 may contribute to delaying the selection of resistant individuals.

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Section: Discussionmentioning

confidence: 99%

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

et al. 2023

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“…Para su control es común la utilización de plaguicidas de amplio espectro, como los organofosforados, peritros y carbamatos (Dubrovsky Berensztein et al, 2017), sin embargo, su uso ocasiona graves conflictos socioambientales (Islam et al, 2021), la mayoría de ellos son insecticidas con efecto residual de lixiviación prolongada, lo que genera perjuicio en el ciclo biogeoquímico (Jin et al, 2019;Pohare et al, 2021). Ya está evidenciado que los insectos tienen la capacidad de desarrollar resistencia genética y eso hace que restrinjan el uso prolongado del mismo ingrediente activo (Datta et al, 2021;Zhang et al, 2020).…”

Section: Introductionunclassified

Bacterias y hongos entomopatógenos en el manejo de Brevicoryne brassicae (Homoptera: Aphididae) en los cultivos de la col

Valverde- Rodríguez,

Cornejo y Maldonado,

Villanueva

et al. 2024

SIEMBRA

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El uso de hongos y bacterias con capacidad patogénica hacia los insectos se considera una alternativa agroecológica sostenible de lucha eficiente contra las plagas por ser microorganismos de fácil producción en escala industrial, formulación y uso; entonces, el objetivo ha sido evaluar cuatro formulados entomopatógenos en el control de Brevicoryne brassicae L, plaga clave de la col (Brassica oleracea L.), en diseño de bloques completos al azar de 4 tratamientos, sumado un testigo absoluto y 4 réplicas, siendo las variables evaluadas la cantidad de pulgones muertos y vivos por planta y cantidad de plantas infestadas. Los formulados comerciales Biosafe, BesT-K, Metarrizo y Yurak que contenían cepas de Bacillus subtilis, Bacillus thuringiensis var kurstaki (Btk), Metarhizium anisopliae y Bauveria bassiana, respectivamente, son los que fueron utilizados mediante la aplicación por aspersión al cultivo en dos épocas de siembra, temporada agosto-diciembre 2019 y agosto-diciembre 2021. Entre los resultados se registró al M. anisopliae con hasta 91,11 % de eficiencia, seguida por B. bassiana con 89,50 % y Bs con 79,38 % en la reducción de las poblaciones en condiciones de campo; en laboratorio el Btk y M. anisopliae alcanzaron el 100 % de mortalidad en un lapso de siete días, por lo que se concluye que a mediano plazo los hongos entomopatógenos en campo resultan ser más eficientes en el control del pulgón, sin embargo, en condiciones controladas el Btk también es eficiente.

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“…In fact, organic agriculture is a holistic way of farming with an aim of conserving the natural resources through the agronomic practices and the use of locally available low cost inputs in order to maintain soil fertility and conserve the rich bio-diversity to provide safe clean water, air and to achieve economical sustainability. Considering these adverse effects of synthetic pesticides on environment and natural habitats and the promotion of environmentally sustainable and organic agriculture, fungicide alternatives such as the use of natural antagonistic microorganisms and natural bioagents such as biopesticides, panchagavya, cow urine, cow urine with plant extracts etc are the effective ways to controlling various plant disease Akhter et al, 2006;Murugan et al, 2012;Rakesh et al, 2013 Pohare et al, 2020) [7][8][9][10][11] . Bacillus and Pseudomonas were among the first bacterial isolates to show promising biocontrol characteristics and promoters of plant health and development and are known to survive in the rhizosphere, with the competence ability to tolerate a reasonable range of abiotic factors including temperature, pH and moisture.…”

Section: Introductionmentioning

confidence: 99%

Isolation and evaluation of cow urine micro-organisms for their antibacterial and antifungal potential

Gawade¹,

Daspute²,

Wagh³

et al. 2020

J Pharmacogn Phytochem

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Cow urine has tremendous applications in agriculture. It is shown to possess inhibitory activity against many phytopathogenic fungi and bacteria. The present study was conducted to determine antibacterial and antifungal efficacy of cow urine. The well-plate assay showed marked growth of inhibition against Aspergilus niger, Fusarium oxysporum, Xanthomonas auxonopodis, Psedomonas solancearum, Xanthomonas cucurbitae by cow urine as well as microbs isolated from cow urine. The TLC and titrimetric analysis were showed presence of lipase enzyme in the cow urine. Moreover, sterilized cow urine can be utilized as natural growth medium for Pseudomonas fluorescence, Bacillus subtilis, mixture of fungi and yeast. The use of cow urine can be the cost-effective and eco-friendly approach for controlling various diseases of plants.

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Bacillus thuringiensis as Potential Biocontrol Agent for Sustainable Agriculture

Cited by 9 publications

References 193 publications

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

Isolation, Genomic, and Proteomic Characterization of a Novel Neotropical Strain of Bacillus thuringiensis with Mosquitocidal Activities

Bacterias y hongos entomopatógenos en el manejo de Brevicoryne brassicae (Homoptera: Aphididae) en los cultivos de la col

Isolation and evaluation of cow urine micro-organisms for their antibacterial and antifungal potential

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