Bacillus thuringiensis and Chlorantraniliprole Trigger the Expression of Detoxification-Related Genes in the Larval Midgut of Plutella xylostella

Shabbir, Muhammad Zeeshan; Yang, Xiangbing; Batool, Raufa; Yin, Fei; Kendra, Paul E.; Li, Zhenyu

doi:10.3389/fphys.2021.780255

Cited by 4 publications

(5 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Lepidoptera: Plutellidae) [8]. It is worth noting that high enzymatic activity does not always associate with resistance to individual insecticides.…”

Section: Resultsmentioning

confidence: 99%

“…It is also provided by the ABC transporters, involved in the excretion of metabolites from the insect body, which were formed in the previous step [7]. Hydrolysis of insecticides by esterases is an important biochemical mechanism for the development of insecticide resistance that is common to several classes of chemical compounds [8][9][10]. Acetylcholinesterase (EC 3.1.1.7, AChE) is a serine esterase of the α-, βhydrolase family.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acetylcholinesterase Activities in Adult Houseflies Musca Domestica L. of the Chlorfenapyr-Resistant Strain

Kinareikina¹,

Silivanova²

2022

Entomol Appl Sci Lett

View full text Add to dashboard Cite

The study of changes that occurred in insect organisms in response to insecticidal exposure and their species-specific characteristics is important for a fuller understanding of the environmental and evolutionary patterns of pesticidal resistance. For chlorfenapyr from the pyrrole group of insecticides, the mechanism underlying the resistance in insects is not quite clearly described. This study evaluated the activity of acetylcholinesterase (AChE) in adults of the house fly Musca domestica of the chlorfenapyrresistant (selected with chlorfenapyr) strain (ChlA). Also, we assessed the kinetic parameters of AChE in females and males of the ChlA strain compared to these of the unselected strain (Lab) of M. domestica for the first time. Specimens of Lab and ChlA strains had no statistically significant differences in specific AChE activity. The percentage remaining activities of propoxur-inhibited AChE was 3.81 times less (р < 0.05) and values of Vmax and Km were 43.3% and 46.9% (р < 0.05), respectively, less in females of the ChlA strain compared to these in females of the Lab strain. For both Lab and ChlA strains M. domestica, the catalytic efficacy of AChE based on Vmax/Km in males was more than that in females. In general, the results obtained suggest that the affinity of AChE to specific ligands (like a substrate acetylthiocholine and an inhibitor propoxur) increased without a rise of the catalytic activity in females of the ChlA strain M. domestica that was under selection with chlorfenapyr during 23-24 generations.

show abstract

“…(Lepidoptera: Plutellidae) [8]. It is worth noting that high enzymatic activity does not always associate with resistance to individual insecticides.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase Activities in Adult Houseflies Musca Domestica L. of the Chlorfenapyr-Resistant Strain

Kinareikina¹,

Silivanova²

2022

Entomol Appl Sci Lett

View full text Add to dashboard Cite

show abstract

“…In studies on Plutella xylostella (diamondback moth) strains resistant to chlorantraniliprole and to Bacillus thuringiensis plus chlorantraniliprole, the expression of numerous potential genes, including cytochrome P450, glutathione S ‐transferase (GST), carboxylesterase and acetylcholinesterase, were found to be increased (Shabbir et al . 2021). A molecular docking study indicated that pesticides could potentially enter a cavity within one of the four subdomains of CAT, highlighting specific amino acid residues and hydrogen bonds involved in CAT–pesticide interactions (Tan et al .…”

Section: Discussionmentioning

confidence: 99%

“…Entomological Research 54 (2024) e12710 (GST), carboxylesterase and acetylcholinesterase, were found to be increased (Shabbir et al 2021). A molecular docking study indicated that pesticides could potentially enter a cavity within one of the four subdomains of CAT, highlighting specific amino acid residues and hydrogen bonds involved in CAT-pesticide interactions (Tan et al 2014).…”

Section: Catalases In Tenebrio Molitormentioning

confidence: 99%

In silico identification and expression analyses of catalases in Tenebrio molitor

JANG,

LEE,

KOJOUR

et al. 2024

Entomological Research

View full text Add to dashboard Cite

Agricultural intensification has led to significant increases in production, but the overuse of pesticides and associated hazards pose threats to biodiversity and ecological functions. Catalase (CAT), a key antioxidant enzyme, plays a crucial role in alleviating oxidative stress by directly interacting with toxins. In this study, we identified three CAT isoforms in Tenebrio molitor (TmCAT‐iso1, TmCAT‐iso2 and TmCAT‐iso3). These CATs possess a CAT domain, tetramer interface sites and a heme‐binding pocket. We examined the expression of Tm catalases across all developmental stages and in specific tissues using quantitative real time polymerase chain reaction (qRT‐PCR) experiments. Our findings demonstrate that TmCAT‐iso1 and TmCAT‐iso3 exhibit peak expression in young and late larval stages, respectively, whereas TmCAT‐iso2 shows peak expression during the egg and pre‐pupal stages. Tissue distribution analysis revealed the high expression of TmCAT‐iso1 and TmCAT‐iso2 in larval hemocytes, whereas TmCAT‐iso3 is predominantly expressed in larval Malpighian tubules. Furthermore, injection with chlorantraniliprole significantly elevated mRNA expression levels of TmCAT‐iso1, TmCAT‐iso2, and TmCAT‐iso3 in larval groups, compared with control groups. Our study highlights the distinct developmental stages and tissues where TmCATs are expressed. We also elucidated the effects of pesticide application on the expression of each TmCAT, revealing the physiological characteristics of CATs in response to these pesticides, which are dose‐ and time‐dependent in T. molitor.

show abstract

“…However, minimal studies on cuticular resistance in invasive insects such as P. xylostella revealed a significant difference in cuticular microRNAs between the chlorantraniliprole resistance population and the susceptible population. They give insight into cuticular alteration during the development of resistance ( Zhu et al, 2017 ; Shabbir et al, 2021 ). There is evidence of cuticular resistance in H. armigera , which successfully prevented deltamethrin adsorption in the body by generating thicker cuticles in resistant individuals compared to susceptible individuals, where insecticide percolation was substantially higher ( Ahmad et al, 2006 ).…”

Section: Resistance In Invasive Insectsmentioning

confidence: 99%

Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

et al. 2023

View full text Add to dashboard Cite

Threatening the global community is a wide variety of potential threats, most notably invasive pest species. Invasive pest species are non-native organisms that humans have either accidentally or intentionally spread to new regions. One of the most effective and first lines of control strategies for controlling pests is the application of insecticides. These toxic chemicals are employed to get rid of pests, but they pose great risks to people, animals, and plants. Pesticides are heavily used in managing invasive pests in the current era. Due to the overuse of synthetic chemicals, numerous invasive species have already developed resistance. The resistance development is the main reason for the failure to manage the invasive species. Developing pesticide resistance management techniques necessitates a thorough understanding of the mechanisms through which insects acquire insecticide resistance. Insects use a variety of behavioral, biochemical, physiological, genetic, and metabolic methods to deal with toxic chemicals, which can lead to resistance through continuous overexpression of detoxifying enzymes. An overabundance of enzymes causes metabolic resistance, detoxifying pesticides and rendering them ineffective against pests. A key factor in the development of metabolic resistance is the amplification of certain metabolic enzymes, specifically esterases, Glutathione S-transferase, Cytochromes p450 monooxygenase, and hydrolyses. Additionally, insect guts offer unique habitats for microbial colonization, and gut bacteria may serve their hosts a variety of useful services. Most importantly, the detoxification of insecticides leads to resistance development. The complete knowledge of invasive pest species and their mechanisms of resistance development could be very helpful in coping with the challenges and effectively developing effective strategies for the control of invasive species. Integrated Pest Management is particularly effective at lowering the risk of chemical and environmental contaminants and the resulting health issues, and it may also offer the most effective ways to control insect pests.

show abstract

Bacillus thuringiensis and Chlorantraniliprole Trigger the Expression of Detoxification-Related Genes in the Larval Midgut of Plutella xylostella

Cited by 4 publications

References 59 publications

Acetylcholinesterase Activities in Adult Houseflies Musca Domestica L. of the Chlorfenapyr-Resistant Strain

Acetylcholinesterase Activities in Adult Houseflies Musca Domestica L. of the Chlorfenapyr-Resistant Strain

In silico identification and expression analyses of catalases in Tenebrio molitor

Insights into insecticide-resistance mechanisms in invasive species: Challenges and control strategies

Contact Info

Product

Resources

About