Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

Zhang, Guoyan; An, Yi; Zabed, Hossain M.; Guo, Qi; Yang, Miaomiao; Yuan, Jun; Li, Wen; Sun, Wenjing; Qi, Xianghui

doi:10.4014/jmb.1807.06066

Cited by 40 publications

(21 citation statements)

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“…Applications of B. subtilis spore surface display B. subtilis has a well-established fermentation and production technology, and spores are resistant to harsh environmental conditions (Wang et al 2011), so the application of spore surface display technology is very extensive. To date, this technology has been used in the production of multimeric proteins, oral vaccine preparations, and industrial enzyme production (Guoyan et al 2019). Table 1 summarizes the related applications of spore surface display of foreign proteins based on recombinant approach in previous studies.…”

Section: Spore Surface Display Of B Subtilis Using Other Anchoring Pmentioning

confidence: 99%

“…Among bacteria, the understanding of its genetic background and physiology is second only to Escherichia coli (Kunst et al 1997;Sonenshein et al 2002). Spore surface display is a method of anchoring exogenous functional proteins on the surface of spores by means of a special structure (Zhang et al 2019). B. subtilis spore surface display has many advantages.…”

Section: Introductionmentioning

confidence: 99%

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Progress in research and application development of surface display technology using Bacillus subtilis spores

Lin

Yuan

et al. 2020

Appl Microbiol Biotechnol

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Bacillus subtilis is a widely distributed aerobic Gram-positive species of bacteria. As a tool in the lab, it has the advantages of nonpathogenicity and limited likelihood of becoming drug resistant. It is a probiotic strain that can be directly used in humans and animals. It can be induced to produce spores under nutrient deficiency or other adverse conditions. B. subtilis spores have unique physical, chemical, and biochemical characteristics. Expression of heterologous antigens or proteins on the surface of B. subtilis spores has been successfully performed for over a decade. As an update and supplement to previously published research, this paper reviews the latest research on spore surface display technology using B. subtilis. We have mainly focused on the regulation of spore coat protein expression, display and application of exogenous proteins, and identification of developing research areas of spore surface display technology.

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Section: Spore Surface Display Of B Subtilis Using Other Anchoring Pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Progress in research and application development of surface display technology using Bacillus subtilis spores

Lin

Yuan

et al. 2020

Appl Microbiol Biotechnol

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“…In recent years, B. subtilis as a good carrier of intestinal immunization has become a hot spot (Permpoonpattana et al ., 2011; Vogt et al ., 2018; Guoyan et al ., 2019; Yao et al ., 2019). Some candidate antigens of C. sinensis were also successfully expressed on the surface of B. subtilis spore and explored their potential as oral vaccines against clonorchiasis (Zhou et al ., 2008; Tang et al ., 2016 a ; Sun et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of immune response toBacillus subtilisspores expressingClonorchis sinensisserpin3

Lin

Sun

et al. 2020

Parasitology

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Clonorchis sinensis (C. sinensis) is one of the most serious food-borne parasites, which can lead to liver fibrosis or cholangiocarcinoma. Effective measures for clonorchiasis prevention are still urgently needed. Bacillus subtilis (B. subtilis) is an effective antigen delivery platform for oral vaccines. Chonorchis sinensis serpin (CsSerpin) was proved to be potential vaccine candidates. In this study, CsSerpin3 was displayed on the surface of B. subtilis spore and recombinant spores were orally administrated to BALB/C mice. CsSerpin3-specific IgA levels in faecal, bile and intestinal mucous increased at 4–8 weeks after the first administration compared with those in control groups. The mucus production and the number of goblet cells in intestinal mucosa elevated in B.s-CotC-CsSerpin3 (CotC, coat protein of B. subtilis spore) spores treated group compared to those in blank control. No significant difference in the activities of glutamic-pyruvic transaminase/ alanine aminotransferase and glutamic oxalacetic transaminase/aspartate aminotransferase were observed between groups. There was no side effect inflammation and observable pathological damage in the liver tissue of mice after administration. Moreover, collagen deposition and Ishak score were statistically reduced in B.s-CotC-CsSerpin3 spores treated mice. In conclusion, B. subtilis spores displaying CsSerpin3 could be investigated further as an oral vaccine against clonorchiasis.

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“…For the surface display of cotA laccase on B. subtilis spores, cotE, cotG, and cotY were selected as anchoring motifs, not only because of their localization on B. subtilis spores, but also due to their relative abundance over other spore coat proteins [34].…”

Section: Construction Of Expression Vector and Recombinant B Subtilimentioning

confidence: 99%

“…CotG is a 24.0 kDa protein [38] that recruits a small subset of proteins to the coat [39]. CotG was selected as an anchor protein for this study because it has been studied extensively [34]. The proteins expressed using CotG as an anchoring motif include streptavidin [29], ω-transaminase [40], chitinase [41], and L-arabinose isomerase [42].…”

Section: Construction Of Expression Vector and Recombinant B Subtilimentioning

confidence: 99%

Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores

Park¹,

Kim²,

Lee³

et al. 2019

Journal of Microbiology and Biotechnology

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Introduction Approximately 10,000 different dyes and pigments are used industrially, and over 7 × 10 5 tons of synthetic dyes are produced annually worldwide [1, 2]. Synthetic dyes are extensively used in textile dyeing, paper printing, color photography, pharmaceutical, food, cosmetics, and other industries [3]. Approximately 128 tons of dye wastewater is discharged daily and high cost is incurred for its removal. Wastewater remediation is challenging because it contains a variety of pollutants and it is difficult to change the composition of the contaminants. Physical, chemical, and biological methods have been widely used to decolorize dye wastewater [4]. The physical and chemical treatment methods are preferred over other methods; however, these methods require high operating cost and other accessory equipment. It would be desirable to develop a new decolorization method that is efficient and cost effective to reduce the overall cost of dye wastewater treatment. Recently, many studies have been published on the decolorization of synthetic dyes by laccase [5, 6]. Laccase was first discovered in 1883 from Rhus vernicifera, the Japanese lacquer tree [7]. Laccases (benzenediol: oxygen oxidoreductase; E.C. 1.10.3.2) are a family of multi-copper oxidases (MCOs) that require oxygen to oxidize organic compounds-particularly phenols and non-phenolic aromatic substrates-by one-electron transfer to form reactive radicals,

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Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications

Cited by 40 publications

References 50 publications

Progress in research and application development of surface display technology using Bacillus subtilis spores

Progress in research and application development of surface display technology using Bacillus subtilis spores

Evaluation of immune response toBacillus subtilisspores expressingClonorchis sinensisserpin3

Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores

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