Bacillus subtilis inhibits growth of  Cladophialophora carrionii in vitro

Romero, Hilda; Viscaya, L.; Ferrara, Giuseppe

doi:10.1016/j.mycmed.2006.01.007

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…species. A study conducted by Romero et al [118] showed that the Bacillus subtilis strain may inhibit the growth of pathogenic fungus Cladophialophora carrionii in vitro.…”

Section: Discussionmentioning

confidence: 99%

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

Mącik

Gryta

Sas-Paszt

et al. 2020

IJMS

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Regarding the unfavourable changes in agroecosystems resulting from the excessive application of mineral fertilizers, biopreparations containing live microorganisms are gaining increasing attention. We assumed that the application of phosphorus mineral fertilizer enriched with strains of beneficial microorganisms contribute to favourable changes in enzymatic activity and in the genetic and functional diversity of microbial populations inhabiting degraded soils. Therefore, in field experiments conditions, the effects of phosphorus fertilizer enriched with bacterial strains on the status of soil microbiome in two chemically degraded soil types (Brunic Arenosol – BA and Abruptic Luvisol – AL) were investigated. The field experiments included treatments with an optimal dose of phosphorus fertilizer (without microorganisms – FC), optimal dose of phosphorus fertilizer enriched with microorganisms including Paenibacillus polymyxa strain CHT114AB, Bacillus amyloliquefaciens strain AF75BB and Bacillus sp. strain CZP4/4 (FA100) and a dose of phosphorus fertilizer reduced by 40% and enriched with the above-mentioned bacteria (FA60). The analyzes performed included: the determination of the activity of the soil enzymes (protease, urease, acid phosphomonoesterase, β-glucosidase), the assessment of the functional diversity of microorganisms with the application of BIOLOGTM plates and the characterization of the genetic diversity of bacteria, archaea and fungi with multiplex terminal restriction fragment length polymorphism and next generation sequencing. The obtained results indicated that the application of phosphorus fertilizer enriched with microorganisms improved enzymatic activity, and the genetic and functional diversity of the soil microbial communities, however these effects were dependent on the soil type.

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“…species. A study conducted by Romero et al [118] showed that the Bacillus subtilis strain may inhibit the growth of pathogenic fungus Cladophialophora carrionii in vitro.…”

Section: Discussionmentioning

confidence: 99%

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

Mącik

Gryta

Sas-Paszt

et al. 2020

IJMS

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“…Gram-positive bacteria such as the ones from Bacillus genera can secrete a variety of substances that are inhibitory to the growth of environmental and phytopathogenic fungi (7)(8)(9)(10). This organism has also been reported to inhibit a fungal chromoblastomycosis (CBM) agent in vitro (11). It has also been reported that Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo

Machado¹,

Anzai²,

Fischman³

2010

J. Venom. Anim. Toxins incl. Trop. Dis

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Abstract:Interactions among microorganisms may be the cause of morphological modifications, particularly in fungal cells. The aim of this work was to examine the changes that occur in cells of the fungus Fonsecaea pedrosoi after in vitro co-culturing with Bacillus subtilis and to explore the results of this interaction in vivo in an experimental murine infection. B. subtilis strain was inoculated into a 15-day pure culture of F. pedrosoi. In vitro, after 48 hours of co-culturing, the fungal cells were roundish. The secretion of fungal dark pigments and production of terminal chlamydoconidia were observed in hyphae after one week. In the in vivo study, two animal groups of 30 BALB/c mice each were employed. One group was inoculated intraperitoneally with hyphal fragments from the co-culture of bacteria and fungi; the other group was infected only with F. pedrosoi hyphae. After seven days of infection, both animal groups developed neutrophilic abscesses. Phagocytosis of bacilli by macrophages occurred at three days. At later periods, generally after 25 days, only roundish cells similar to sclerotic bodies remained in the tissues while hyphae were eliminated by 15 to 20 days. These fungal forms originated mainly from terminal chlamydoconidia. The co-culturing between bacteria and fungi may constitute a mechanism to rapidly obtain resistant fungal forms for host defenses, especially for chromoblastomycosis (CBM) experimental infections. Key words:Bacillus subtilis, Fonsecaea pedrosoi, microbial interaction, co-culture, fungal antagonism. In a preliminary study, we isolated an environmental gram-positive bacterium from Brazilian soil that antagonizes several fungi, including some dematiaceous species (23). Herein, we studied the interactions between this bacterium and a clinical strain of F. pedrosoi in two manners: firstly, we examined the in vitro changes in fungal cells after co-culturing with bacillus, and secondly, we explored the in vivo significance of this interaction. Original PaPer MATERIALS AND METHODS AnimalsTwo groups of 30 male BALB/c mice each weighing about 23 g (6 to 8-week-old), were used throughout this study. The specific-pathogenfree animals were purchased from CEDEME/ UNIFESP (Brazil). The protocol used was approved by the UNIFESP Ethics Committee under project number 0808/05. MicroorganismsF. pedrosoi strain (EPM -380/03) was isolated from a patient with CBM examined in the Dermatology Outpatient Department, UNIFESP, in 2003. It was cultivated on Sabouraud dextrose agar (SDA -Difco Laboratories, USA) supplemented with 80 mg/L gentamycin at a temperature of 28°C, with periodic transfers at 15-day intervals into a new SDA medium containing antibiotics. An environmental bacillus isolated from random soil samples collected from the campus of the Federal University of Mato Grosso (Cuiabá, Mato Grosso state, Brazil) was used in this study; as in previous co-culture assays, it was able to inhibit the growth of some phytopathogenic fungi and to induce the release of dark pigment by dematiaceous molds. ...

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“…This species is an antagonist for many mould fungi suppressing their growth in both in vitro and in vivo. It is effective against Alternaria solani, Monilia fructicola (McKeen et al 1986), Botrytis cinerea (Helbig and Bochow 2001;Touré et al 2004), Cladophialophora carrionii (Romero et al 2006), Fusarium sp. (Chan et al 2003;Dolej and Bochow 1996), Eutipa lata (Ferreira et al 1991), Gaeumannomyces graminis var.…”

Section: Introductionmentioning

confidence: 99%

Characteristics and antimicrobial activity of Bacillus subtilis strains isolated from soil

Todorova

Kozhuharova

2009

World J Microbiol Biotechnol

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Antagonistic Bacillus strains were isolated from soil and analyzed for the purpose of determining whether they could be used as natural biological agents. Primary in vitro screening for antagonism of the isolates was performed against five phytopathogenic mould fungi. Strains TS 01 and ZR 02 exhibited the most pronounced inhibitory effects. They were identified as Bacillus subtilis on the basis of their morphological, cultural and physiology-biochemical properties as well as their hierarchical cluster analysis conducted by means of computer program SPSS. The antimicrobial activity of the strains from cultural medium and sterile filtrate were determined in vitro against a great number of predominantly phytopathogenic fungi and bacteria. TS 01 and ZR 02 strains exhibited very broad and at the same time degree varying antibiotic spectra of activities against both Gram-positive and Gram-negative microorganisms. Many of them were tested against sensitivity to the antimicrobial action of B. subtilis for the very first time. B. subtilis TS 01 and ZR 02 showed highest antifungal activity (sterile zone in diameter over 37 mm) against Alternaria solani, Botrytis cinerea, Monilia linhartiana 869, Phytophthora cryptogea 759/1 and Rhizoctonia sp. The most sensitive bacterial species were found to be Pseudomonas syringae pv. tomato Ro and Xanthomonas campestris with sterile zones 48.0 and 50.0 mm in diameter, respectively. The latter draws a conclusion that the isolated and identified Bacillus subtilis strains are promising natural biocontrol agents and should be further studied and tested for control of numerous plant diseases.

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Bacillus subtilis inhibits growth of Cladophialophora carrionii in vitro

Cited by 3 publications

References 5 publications

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

Bacillus subtilis induces morphological changes in Fonsecaea pedrosoi in vitro resulting in more resistant fungal forms in vivo

Characteristics and antimicrobial activity of Bacillus subtilis strains isolated from soil

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