Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits

Guo, Mengjiao; Wu, Fahao; Hao, Guangen; Qi, Qin; Li, Rong; Li, Ning; Wei, Liangmeng; Chai, Tongjie

doi:10.3389/fimmu.2017.00354

Cited by 263 publications

(163 citation statements)

References 45 publications

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“…The correlation analysis showed that the relative abundances of Lachnospiraceae_XPB1014_group, Rikenellaceae_RC9_gut_group, Ruminococcaceae_UCG-002 and Prevotella_2 were negatively correlated with serum IgG content; the lower abundances of these genera triggered by CPB addition indicated that CPB was able to increase host immunity, corresponding with the high serum IgG concentration caused by CPB administration. The previous report showed that probiotics could increase host serum IgG levels [30,31], in agreement with this study. This study demonstrated that oral administration of CPB enhanced host intestinal homeostasis by modulating the composition of gut microbiota for improving piglet production performance.…”

Section: Discussionsupporting

confidence: 93%

Dietary Supplementation with Compound Probiotics and Berberine Alters Piglet Production Performance and Fecal Microbiota

Yang

Chang

et al. 2020

Animals

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Simple Summary: In order to find antibiotic substitutes for weaned piglet health and growth, compound probiotics and berberine (CPB) were selected in this study. The results indicated that CPB could replace antibiotics to improve piglet health and decrease mortality, diarrhea and rejection rates. CPB was also able to regulate fecal microbiota as well as improve protein digestibility and serum biochemical parameters. Therefore, CPB might be a good antibiotic alternative in piglet production performance.Abstract: This study was conducted to investigate the effects of dietary supplementation with compound probiotics and berberine (CPB) on growth performance, nutrient digestibility and fecal microflora in weaned piglets. A total of 200 piglets 35 days old were randomly allocated to 5 groups, 4 replications in each group, and 10 piglets in each replication. Group A was the basal diet; group B was supplemented with antibiotics and zinc oxide; groups C, D and E were supplemented with 0.06%, 0.12% and 0.18% CPB, respectively. The experimental period was 42 d. The results indicated that there were no significant differences in average daily feed intake (ADFI), average daily gain (ADG) and feed conversion rate (FCR) among five groups (p > 0.05). However, mortality, diarrhea and rejection rates in the control group were higher than that in other groups. CPB could increase protein digestibility and serum IgG content (p < 0.05), while it could decrease serum urea nitrogen content and alkaline phosphatase activity (p < 0.05). Analysis of fecal microbiota showed that the relative abundances of Bacteroides and Firmicutes were increased, while the relative abundances of opportunistic pathogens such as Spirochaetae and Protebactreria were dramatically decreased in piglets fed with CPB or antibiotics, compared with the control group. Furthermore, CPB intervention increased the relative abundances of Prevotella_9, Megasphaera and Prevotella_2, while decreased the relative abundance of Prevotellaceae_NK3B31_group. Correlation analysis revealed that there was good correlation between serum indexes and fecal microbiota. It was suggested that CPB might be a promising antibiotic alternative for improving piglet health and immunity, decreasing mortality by positively altering gut microbiota.

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Section: Discussionsupporting

confidence: 93%

Dietary Supplementation with Compound Probiotics and Berberine Alters Piglet Production Performance and Fecal Microbiota

Yang

Chang

et al. 2020

Animals

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“…Crucially, integration of the expression systems into the genomes of the engineered microorganisms will help diminish the spreading of antibiotic resistant genes to pathogens via the plasmids. To address further safety concerns, we envision that the platform could be adapted to other microorganisms that previously demonstrated their innocuity for humans, and were used in other animal models for synthetic biology applications, such as E. coli Nissle 1917 strain (EcN) [38] or Bacillus subtilis [39]. As such, this work constitutes an important methodological improvement in the field of living functional materials and living material foundries, and could have implications for therapeutic applications.…”

Section: Discussionmentioning

confidence: 99%

Engineered Living Materials based on Adhesin-mediated Trapping of Programmable Cells

Guo¹,

Dubuc²,

Rave

et al. 2019

Preprint

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5 Stichting PAMM, Laboratory for pathology and medical microbiology, De Run 6250, 5504 DL Veldhoven 6 Laboratory of Physical Chemistry 7 Molecular biosensing for medical diagnostics 8 Laboratory of protein engineering 1-4, 6-8 : Abstract:Engineered living materials have the potential for wide-ranging applications such as biosensing and treatment of diseases. Programmable cells provide the functional basis for living materials, however, their release into the environment raises numerous biosafety concerns. Current designs that limit the release of genetically engineered cells typically involve the fabrication of multi-layer hybrid materials with sub-micron porous matrices. Nevertheless the stringent physical barriers limit the diffusion of macromolecules and therefore the repertoire of molecules available for actuation in response to communication signals between cells and their environment. Here, we engineer a first-of-its-kind living material entitled 'Platform for Adhesin-mediated Trapping of Cells in Hydrogels' (PATCH). This technology is based on engineered E. coli that displays an adhesion protein derived from an Antarctic bacterium with high affinity for glucose. The adhesin stably anchors E. coli in dextran-based hydrogels with large pore diameters (10-100 µm) and reduces the leakage of bacteria into the environment by up to 100-fold. As an application of PATCH, we engineered E. coli to secrete lysostaphin via the Type 1 Secretion System and demonstrated that living materials containing this E. coli inhibit the growth of S. aureus, including the strain resistant to methicillin (MRSA). Our tunable platform allows stable integration of programmable cells in dextran-based hydrogels without compromising free diffusion of macromolecules and could have potential applications in biotechnology and biomedicine. Introduction:Synthetic biology aims to design programmable cells that combine sensing and molecular computing operations with on-demand production of proteins that have a broad spectrum of therapeutic applications [1][2][3][4]. Engineered living materials (ELMs) integrate genetically engineered cells with free standing materials and represent a new class of environmentally responsive living devices with designer physicochemical and material properties [5][6][7]. Ideally, ELMs provide mechanical robustness to engineered cells, prevent their leakage to the environment and allow cells to be viable for extended periods of time. The containment of genetically-modified microorganisms (GMMs) within various materials has become a grand challenge for future synthetic biology applications [8]. To date, strategies for containing GMMs inside a living device are based on the physical confinement by multi-layer materials [9][10][11]. Hybrid micro-patterned devices combining layers of elastomer and microporous hydrogel enabled the exchange of information with surrounding environment via diffusion of chemical inducers and their sensing by GMMs while displaying high mechanical resilience [10]. Nevertheless, the low porosity ...

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“…possess high secretory ability and some strains utilize ''cell factories" to produce industrial enzymes (Ruiz-Garcia et al, 2005; Niazi et al, 2014). Several B. subtilis strains can colonize the intestinal microvilli of the animal, support nutrition, and trigger a healthy immune system (Guo et al, 2016, 2017; Abd El-Tawab et al, 2016). Several strains of B. amyloliquefaciens subsp.…”

Section: Genes Involved In Promoting Animal Growthmentioning

confidence: 99%

“…The most commonly used probiotics are Lactobacillus , Bifidobacterium , Bacillus , and yeast strains. Supplemental B. subtilis increases growth performance, disease resistance and immune response in animals (Guo et al, 2016, 2017). Previous reports have demonstrated that Bacillus velezenis QST713 (Pandin et al, 2018), Bacillus velezenis M75 (Kim et al, 2017), and Bacillus velezenis S3-1 (Jin et al, 2017) showed a strong ability to form biofilm and inhibit pathogens.…”

mentioning

confidence: 99%

Complete genome sequence of Bacillus velezensis JT3-1, a microbial germicide isolated from yak feces

Y¹,

X²,

Jia³

et al. 2019

Preprint

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15 Bacillus velezensis JT3-1 is a probiotic strain isolated from feces of the domestic yak (Bos 16 grunniens) in the Gansu province of China. It has strong antagonistic activity against Listeria 17 monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, Mannheimia 18 haemolytica, Staphylococcus hominis, Clostridium perfringens, and Mycoplasma bovis. These 19properties have made the JT3-1 strain the focus of commercial interest. In this study, we describe 20 the complete genome sequence of JT3-1, with a genome size of 3,929,799 bp, 3761 encoded genes 21 and an average GC content of 46.50%. Whole genome sequencing of Bacillus velezensis JT3-1 22 will lay a good foundation for elucidation of the mechanisms of its antimicrobial activity, and for 23 its future application. 24Keywords: Bacillus velezensis JT3-1; Genome; Antimicrobial activity; Secondary metabolite 25Province, China. Our study showed that the B. velezensis JT3-1 strain had strong antagonistic 43 activity against various intestinal pathogenic flora, and stimulation of animal (such as sheep, goat, 44 mouse, pig and cattle) growth (not published). However, there are no published reports on the 45 novel B. velezensis JT3-1 strain and its genome sequence is not found in Genebank. In this study, 46 the genome sequence of the novel strain JT3-1 was determined, the secondary metabolite clusters 47 Pandin et al., 2018). This analysis showed that at least 17% of the JT3-1 genome contributed to 99

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Bacillus subtilis Improves Immunity and Disease Resistance in Rabbits

Cited by 263 publications

References 45 publications

Dietary Supplementation with Compound Probiotics and Berberine Alters Piglet Production Performance and Fecal Microbiota

Dietary Supplementation with Compound Probiotics and Berberine Alters Piglet Production Performance and Fecal Microbiota

Engineered Living Materials based on Adhesin-mediated Trapping of Programmable Cells

Complete genome sequence of Bacillus velezensis JT3-1, a microbial germicide isolated from yak feces

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