Bacillus species (BT42) isolated from Coffea arabica L. rhizosphere antagonizes Colletotrichum gloeosporioides and Fusarium oxysporum and also exhibits multiple plant growth promoting activity

Abstract

Background Colletotrichum and Fusarium species are among pathogenic fungi widely affecting Coffea arabica L., resulting in major yield loss. In the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing Colletotrichum gloeosporioides and Fusarium oxysporum as well as promotes plant growth.ResultsA total of 42 Bacillus species were isolated, one of the isolates named BT42 showed maximum radial mycelial growth inhibition against Colletotrichum gloeosporioides (78%) and Fusarium oxysporum (86%). BT42 increased germination of Coffee arabica L. seeds by 38.89%, decreased disease incidence due to infection of Colletotrichum gloeosporioides to 2.77% and due to infection of Fusarium oxysporum to 0 (p < 0.001). The isolate BT42 showed multiple growth-promoting traits. The isolate showed maximum similarity with Bacillus amyloliquefaciens.Conclusion Bacillus species (BT42), isolated in the present work was found to be capable of antagonizing the pathogenic effects of Colletotrichum gloeosporioides and Fusarium oxysporum. The mechanism of action of inhibition of the pathogenic fungi found to be synergistic effects of secondary metabolites, lytic enzymes, and siderophores. The major inhibitory secondary metabolite identified as harmine (β-carboline alkaloids).

Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents

“…The basis for the observed activity of Bacillus spp. against Fusarium pathogenic species has been linked to secondary metabolites that strongly interfere with fungal pathogens [74,75]. To test the validity of our strategy against emerging pathogens, we isolated Fusarium sp.…”

Coffee Microbiota and Its Potential Use in Sustainable Crop Management. A Review

“…Coffee-associated microorganisms have been extensively studied for their potential use as plant-growth promoting agents including rhizospheric bacteria and fungi (Jimenez-Salgado et al, 1997;Posada et al, 2013;Kejela et al, 2016;Perea Rojas et al, 2019), epiphytic bacteria (Estrada-De Los Santos et al, 2001;Teshome et al, 2017), endophytic bacteria (Jimenez-Salgado et al, 1997;Silva et al, 2012) and AMF (Caldeira et al, 1983;Perea Rojas et al, 2019).…”

Mecanismos de acción de <i>Bacillus</i> spp. (Bacillaceae) contra microorganismos fitopatógenos durante su interacción con plantas

“…Las biopelículas pueden ser consideradas como capas compactas relativamente uniformes, formadas por el crecimiento concertado y organizado de microorganismos. La formación de estas biopelículas les permite a los agentes de control biológico ser buenos colonizadores (Posada et al, 2018), ya que les proporciona una ventaja adaptativa al proteger a la población de condiciones ambientales adversas y otros factores mecánicos, lo que puede conducir además a la exclusión de nicho de los patógenos (Lalloo et al, 2010;Rafique et al, 2015).…”

“…En este sentido se ha demostrado que la cepa de Bacillus sp. BT42 disminuye la incidencia de Colletotrichum gloeosporoides y F. oxysporum en plántulas de café (Coffea arabica L.) a 2,8 % y 0 % en relación con los controles de enfermedad que presentaron una incidencia de 91,7 % y 88,9 %, debido a la producción -1,3-glucanasas (Kejela et al, 2016).…”