Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusarium graminearum and Bipolaris sorokiniana

Shali, Abbas; Ghasemi, Seyedhadi; Ahmadian, Gholamreza; Ranjbar, Gholam Ali; Dehestani, Ali; Khalesi, Naeimeh; Motallebi, Ebrahim; Vahed, Majid

doi:10.1007/s12600-009-0078-8

Cited by 35 publications

(13 citation statements)

References 33 publications

(36 reference statements)

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“…Since our proteomics studies did not shed light on the possible cell wall anchoring of the authentic YhcR by sortase, we investigated whether the cell-wall-anchoring domain of YhcR can facilitate the binding of a reporter protein to the cell wall of B. subtilis. For this purpose, the secreted chitinase ChiS from Bacillus pumilus was used [30][31][32][33]. Notably, the yhcR and yhcS genes are co-expressed, which suggests that YhcR might be a natural substrate for YhcS especially since sortases are often co-expressed with a cognate substrate protein.…”

Section: Yhcs-mediated Wall Attachment Of a Chitinase Reporter Proteimentioning

confidence: 99%

Functional analysis of the sortase YhcS in Bacillus subtilis

et al. 2011

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Sortases of Gram-positive bacteria catalyze the covalent C-terminal anchoring of proteins to the cell wall. Bacillus subtilis, a well-known host organism for protein production, contains two putative sortases named YhcS and YwpE. The present studies were aimed at investigating the possible sortase function of these proteins in B. subtilis. Proteomics analyses revealed that sortase-mutant cells released elevated levels of the putative sortase substrate YfkN into the culture medium upon phosphate starvation. The results indicate that YfkN required sortase activity of YhcS for retention in the cell wall. To analyze sortase function in more detail, we focused attention on the potential sortase substrate YhcR, which is co-expressed with the sortase YhcS. Our results showed that the sortase recognition and cell-wall-anchoring motif of YhcR is functional when fused to the Bacillus pumilus chitinase ChiS, a readily detectable reporter protein that is normally secreted. The ChiS fusion protein is displayed at the cell wall surface when YhcS is co-expressed. In the absence of YhcS, or when no cell-wall-anchoring motif is fused to ChiS, the ChiS accumulates predominately in the culture medium. Taken together, these novel findings show that B. subtilis has a functional sortase for anchoring proteins to the cell wall.

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Section: Yhcs-mediated Wall Attachment Of a Chitinase Reporter Proteimentioning

confidence: 99%

Functional analysis of the sortase YhcS in Bacillus subtilis

et al. 2011

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“…These fungi can establish on the seed surface, and colonize and reproduce in the rhizosphere (Carvalho et al, 2011a). In recent years, biofungicides have been developed and commercialized worldwide (Shali et al, 2010). Due to the negative aspects involved in the use of agrochemicals such as contamination of the environment with toxic residues that may affect humans and animals (Carvalho et al, 2007), the use of biofungicides in seed treatments has been expanded to many crops (Corrêa et al, 2008), which is true especially in Brazil where Trichoderma spp.…”

Section: Introductionmentioning

confidence: 99%

Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment

Carvalho

Lobo

Martins

et al. 2014

Trop. plant pathol.

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Biological control of seed-borne pathogens has shown to enhance germination and physiological quality of seeds. The objectives of this study were to evaluate the in vitro antagonistic effect of five Trichoderma harzianum isolates (CEN287, CEN288, CEN289, CEN290, and CEN316) against Fusarium oxysporum f. sp. phaseoli (Foxy) and test its potential use in seed treatment. Initially, pathogen and antagonists were grown in paired cultures at 25ºC, from which samples were assessed using scanning electron microscopy (SEM). Then, clean or Foxy-infected seeds were treated with conidial suspension of the antagonists. Percent of Foxy-infected seeds and normal seedlings were evaluated at seven and nine days of incubation, respectively. All but one Trichoderma isolate (CEN290) inhibited Foxy mycelial growth. SEM analysis revealed that only one Trichoderma isolate (CEN287) showed parasitic interaction with Foxy. Two isolates (CEN287 and CEN316) significantly reduced the Foxy incidence and enhanced seed germination, though less effective than the fungicide mixture (carboxin + thiram). A principal component analysis indicated the importance of volatile metabolites in reducing Foxy incidence on common bean seeds. CEN287 Trichoderma harzianum isolate formed a single group due to its increase in germination rate of Foxyinfected seeds.

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“…and Trichoderma spp. can grow in plants, some of them as parasitic organisms (Fisher et al 1996;Fröhlich and Hyde 1999;Hashizume et al 2008;Shali et al 2010;Suryanarayanan et al 2009;Vázquez de Aldana et al 2013). The functional role of the mycobiota present in leaf-cutting ant colonies is still not known.…”

Section: D��mentioning

confidence: 99%

Diversidad de micobiota en diferentes especies de hormigas cortadoras de hojas y sitios de Argentina

Marfetán

Folgarait

2017

Ecol. Austral

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A��. A�ine ants are a monophyletic group comprising more than 230 species, distributed exclusively in the New World. All higher A�ini ants depend on the cultivation of fungus gardens for food, and those gardens are continuously exposed to alien microorganisms. The present work describes, for the first time, the composition, relative frequency and the richness of the fungal species comprising the mycobiota from the garden of the most abundant Acromyrmex species from different regions of Argentina. We also compared, using a multivariate approach, the mycobiota composition with the purpose of testing two hypotheses: 1) whether mycobiota is defined by the ant species, or 2) whether mycobiota is determined by the geographical region. We found that each fungal community had a particular composition and abundance: the species that were highly frequent in a community or site, could be rare or altogether absent in another community or site. Species richness, as well as the theoretical maximum richness, also changed with locations. Furthermore, we found that different ant species at the same site had similar mycobiotas, whereas the same ant species in distant locations had only a few fungal species in common. Therefore, we concluded that mycobiota composition changed considerably with geographical site and was not dependent on the ant species. Our results provide an additional understanding of the leaf cu�ing-ants system, confirming that the garden of leaf cu�ing ants nests is a complex and dynamic fungal community which is dependent on the site where it was located.[Keywords: abundance, Acromyrmex, Ascomycota, fungal communities, richness, Zygomycetes] R��. Diversidad de micobiota en diferentes especies de hormigas cortadoras de hojas y sitios de Argentina. Las hormigas de la familia A�ini son un grupo monofilético que incluye más de 230 especies distribuidas exclusivamente en el Nuevo Mundo. Todas las hormigas A�ini superiores se alimentan de un hongo que cultivan dentro de sus nidos, y los cultivos de este hongo se encuentran continuamente expuestos a microorganismos exógenos a la colonia. Este trabajo describe por primera vez la composición, la frecuencia relativa y la riqueza de las especies que conforman las micobiotas encontrada en los jardines de las especies del género Acromyrmex más abundantes en diferentes regiones de la Argentina. También comparamos, mediante métodos multivariados, la composición de la micobiota con el propósito de poner a prueba dos hipótesis: 1) la micobiota está definida por las especies de hormigas, o 2) la micobiota cambia en función de la región geográfica. Se pudo observar que cada comunidad fúngica tuvo una composición y una abundancia de especies particular; las especies más frecuentes en un sitio o comunidad fue una especie rara o poco frecuente en otra comunidad o sitio. La riqueza de especies y la riqueza máxima teórica también cambiaron con los sitios. Además, encontramos que diferentes especies de hormigas en el mismo sitio tuvieron micobiotas similares, mientras que las...

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Bacillus pumilus SG2 chitinases induced and regulated by chitin, show inhibitory activity against Fusarium graminearum and Bipolaris sorokiniana

Cited by 35 publications

References 33 publications

Functional analysis of the sortase YhcS in Bacillus subtilis

Functional analysis of the sortase YhcS in Bacillus subtilis

Biological control of Fusarium oxysporum f. sp. phaseoli by Trichoderma harzianum and its use for common bean seed treatment

Diversidad de micobiota en diferentes especies de hormigas cortadoras de hojas y sitios de Argentina

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