Bacillus NP5 Improves Growth Performance and Resistance Against Infectious Myonecrosis Virus in White Shrimp (Litopenaeus vannamei)  (Bacillus NP5 Meningkatkan Pertumbuhan dan Ketahanan Terhadap Infeksi Virus Myonecrosis  pada Udang Putih (L. vannamei)

Widanarni, Widanarni; Yuhana, Munti; Arief, Muhammad

doi:10.14710/ik.ijms.19.4.211-218

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…During the culture period, the experiment showed that THIO encapsulation ensured a high production rate and THIO viability after 123 days of storage. Observations from this present study suggest that THIO incorporated into commercial pellet feed has a bene cial effect on growth performance nal weight, weight gain and FCR (Ghosh, et al, 2016;Yu, et al, 2021;Widanarni, et al, 2014;Putra, et al, 2017;Shao, et al, 2017). This is an identical encouraging result for the farmers involved in shrimp farming in association with THIO, as the quantity of shrimp is related to the market price and foreign exchange earnings (Ghosh, et al, 2016;Widanarni, et al, 2014).…”

Section: Discussionsupporting

confidence: 70%

“…Observations from this present study suggest that THIO incorporated into commercial pellet feed has a bene cial effect on growth performance nal weight, weight gain and FCR (Ghosh, et al, 2016;Yu, et al, 2021;Widanarni, et al, 2014;Putra, et al, 2017;Shao, et al, 2017). This is an identical encouraging result for the farmers involved in shrimp farming in association with THIO, as the quantity of shrimp is related to the market price and foreign exchange earnings (Ghosh, et al, 2016;Widanarni, et al, 2014). In the present study, the survival rate of shrimp was higher in THIO-fed farms than in the control group and agreed with previously report (Tsai, 2019;Yu, et al, 2021).…”

Section: Discussionmentioning

confidence: 59%

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Impact of THIONIL ® on reducing water and soil micropollutant and enhancement of growth, survival, antivibrio, immunity and health indices of Litopenaeus vannamei in pond trial

Thamarai

Sivaprakasam

Harikrishnan

et al. 2023

Preprint

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There is an urgent requisite for an improvement of the white leg shrimp Litopenaeus vannamei health related indices and immunity due to the emerging diseases. Recently, probiotics have been playing an important role in L. vannamei health management. Therefore, the current pond trail was to evaluate the probiotic proficiency of commercial probiotic product of THIONIL (THIO) on enhancement of the water, soil, growth, digestibility, survival, immune related indices and susceptibility of L. vannamei pond level trials. The study was carried out in the major shrimp culturing regions of Kavali, Nellore and Ponneri, Tamil Nadu, India. The experimental L. vannamei was divided into seven groups (1Lacks/ha), such as, negative control (THIO 0% - untreated), positive control (challenge with vibriosis) 2%, 4%, 6%, 8%, and 10% of THIO was encapsulated with commercial feed (CP Aqua). Bioassays were carried out in different day intervals includes, 0, 5, 25, 50, 100 and 123 for evaluating the productivity, anti-vibrio activity, digestive enzyme for digestibility, histological, immunological indices, and cytotoxicity in Artemia nauplii. The significant differences were observed in the increased growth (35.71 g/ shrimp) and digestive parameters in 10% THIO-fed shrimp, however, the other THIO-fed shrimp groups were also showed notable growth compared with control group. The results revealed that, the gill, hepatopancreas and gastro intestine have ha lesser tissue damage in 10% THIO when compared with control. The 10% THIO fed exhibited resistant potential against Vibrio parahaemolyticus (0.008 × 104 cfu/g) (AHPND-Acute Hepatopancreatic Necrosis Disease) and Vibrio harveyi (0.051×105 cfu/g) (Vibriosis). Additionally, 10% THIO fed shrimp revealed significant enhancement of immune related parameters when compared with control. In conclusion the results documented the THIO treatment shrimp had significant enhancement in the water and soil quality growth, strengthens the innate immunity and showed protection against Vibrio infections.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 59%

Impact of THIONIL ® on reducing water and soil micropollutant and enhancement of growth, survival, antivibrio, immunity and health indices of Litopenaeus vannamei in pond trial

Thamarai

Sivaprakasam

Harikrishnan

et al. 2023

Preprint

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“…This study used Bacillus sp. NP5 has been tested to increase growth performance, immune response, and survival as well as inhibit the growth of pathogenic bacteria in several aquaculture such as vannamei shrimp against Infectious Myonecrosis Virus infection (Widanarni et al, 2014), carp against infection A. hydrophila (Djauhari et al, 2016), tilapia against Streptococcus agalactiae infection ( Agung et al, 2015), and catfish against A. hydrophila infection (Tamamdusturi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp.

Sudrajat,

Yuhana,

Widanarni

et al. 2023

Jurnal Akuakultur Indonesia

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Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P<0.05) higher probiotic stimulation value than other doses. In the in vivo test, the study used a completely randomized design with five treatments, namely simplicia (DOSP 20 g/kg), probiotic (PRO, Bacillus sp. NP5 108 CFU/mL, 1% v/w), combination (PRO+DOSP), and control (positive and negative). Fish were reared for 45 days and fed three times a day. On day 46, fish from all treatments, except negative control, were infected with an A. hydrophila dose of 106 CFU/mL injected intramuscularly. The results showed that the combination treatment (PRO+DOSP) gave better total erythrocytes, hemoglobin, hematocrit, total leukocytes and phagocytosis activity than probiotics, DOSP, and control. Administering the combination (PRO+DOSP) can reduce the total number of A. hydrophila lower than the probiotic, DOSP, and control treatments. In addition, the survival rate of catfish in the combined treatment (PRO+DOSP) was significantly (P<0.05) higher than probiotics, DOSP, and control. The results of this study can be a helpful reference and application for the early prevention of A. hydrophila infection. Keywords: aquaculture, Bacillus sp. NP5, dayak onion, probiotics, simplicia powder ABSTRAK Aeromonas hydrophila adalah penyebab utama penyakit bercak merah pada budidaya ikan lele dan menyebabkan kerugian ekonomi cukup besar pada akuakultur Indonesia. Penelitian ini bertujuan untuk mengevaluasi aktivitas prebiotik serbuk simplisia bawang dayak (SSBD) dan pengaruh penambahan kombinasi probiotik Bacillus sp. NP5 dan SSBD pada pakan terhadap respons imun dan kelangsungan hidup ikan lele yang diinfeksi A. hydrophila. Lima dosis (0,20, 0,25, 0,30, 0,35, dan 0,40 g/mL) SSBD diuji secara in vitro untuk menilai skor aktivitas prebiotik. Hasil penelitian menunjukkan bahwa dosis 0,20 g/mL memberikan nilai stimulasi probiotik signifikan (P<0,05) lebih tinggi dibandingkan dosis lainnya. Pada uji in vivo, penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan, yaitu simplisia (SSBD, serbuk simplisia bawang dayak 20 g/kg), probiotik (PRO, Bacillus sp. NP5 108 CFU/mL, 1% (v/w), kombinasi (PRO+SSBD), dan kontrol (positif dan negatif). Ikan dipelihara selama 45 hari dan diberi pakan tiga kali sehari. Pada hari ke 46, ikan pada semua perlakuan, kecuali kontrol negatif, di infeksi A. hydrophila dosis 106 CFU/mL secara intramuskular. Hasil penelitian menunjukkan bahwa kombinasi (PRO+SSBD) memberikan total eritrosit, hemoglobin, hematokrit, total leukosit dan aktivitas fagositosis lebih baik dibandingkan probitoik, SSBD, dan kontrol. Kombinasi (PRO+SSBD) mampu menekan total A. hydrophila lebih rendah dibandingkan probiotik, SSBD, dan kontrol. Selain itu, tingkat kelangsungan hidup ikan lele pada perlakuan kombinasi (PRO+SSBD) signifikan (P<0,05) lebih tinggi dibandingkan probitoik, SSBD, dan kontrol. Hasil penelitian ini bisa menjadi referensi dan aplikasi yang efektif untuk pencegahan dini infeksi A. hydrophila. Kata kunci: akuakultur, Bacillus sp. NP5, bawang dayak, probiotik, serbuk simplisia

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“…5 Production yield is often plunged by diseases, thus incurring a massive financial burden to the industry. 6 Some of the diseases associated with shrimp farming include acute hepatopancreatic necrosis disease (AHPND), 7 running mortality syndrome, loose shell syndrome, white faecal syndrome, 8 white spot syndrome, 9,10 infectious myonecrosis, 11 infectious hypodermal and haematopoietic necrosis virus infection, 12 Taura syndrome, 13 viral convert mortality disease, white tail disease, yellowhead disease, 14 and the hepatopancreatic microsporidiosis caused by the microsporidian parasite, Enterocytozoon Hepatopenaei. 15 The diseases have an even higher impact in monoculture systems where a single shrimp species is cultured throughout all seasonal cycles.…”

Section: Introductionmentioning

confidence: 99%

Harnessing the potentialities of probiotics, prebiotics, synbiotics, paraprobiotics, and postbiotics for shrimp farming

Goh

Tan

Law

et al. 2022

Reviews in Aquaculture

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The shrimp farming industry is receiving increasing attention globally. However, extending the frontiers of the industry has been a challenge as high stocking density often increases susceptibility to diseases and compromises shrimp quality. There has been a huge adoption of bacterial‐derived additives in recent decades, particularly in Asia. Accumulating evidence demonstrates the modulatory effect of these functional additives on the animal gut microbiota composition, thus making them potential alternatives to antibiotics in controlling infectious diseases, such as acute hepatopancreatic necrosis disease (AHPND). These functional additives could be introduced in various forms, such as probiotics, synbiotics, paraprobiotics, and postbiotics. This article aims to critically review previous studies that investigated the effects of these bacterial derivatives, particularly on shrimp species. The surge of research in recent years indirectly attested to the immense potentialities of these functional additives. Generally, the introduction of bacterial derivatives not only helped in disease control but also demonstrated encouraging outcomes, including better survival rate, immune enhancement, and growth promotion effects. It is interesting to note that the effectiveness of probiotics extended beyond the viability of the bacteria, thus leading to concepts such as paraprobiotics and postbiotics. Although this is a relatively new research area with limited information, this review aims to consolidate available data and provide a comprehensive overview of the state of the art of this research arena. The challenges in each research domain were also discussed towards the end of this review to provide insights into potential future research niches.

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Bacillus NP5 Improves Growth Performance and Resistance Against Infectious Myonecrosis Virus in White Shrimp (Litopenaeus vannamei) (Bacillus NP5 Meningkatkan Pertumbuhan dan Ketahanan Terhadap Infeksi Virus Myonecrosis pada Udang Putih (L. vannamei)

Cited by 9 publications

References 20 publications

Impact of THIONIL ® on reducing water and soil micropollutant and enhancement of growth, survival, antivibrio, immunity and health indices of Litopenaeus vannamei in pond trial

Impact of THIONIL ® on reducing water and soil micropollutant and enhancement of growth, survival, antivibrio, immunity and health indices of Litopenaeus vannamei in pond trial

Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp.

Harnessing the potentialities of probiotics, prebiotics, synbiotics, paraprobiotics, and postbiotics for shrimp farming

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