Bacillus natto ameliorates obesity by regulating PI3K/AKT pathways in rats

Sun, Ruiyue; Li, Da; Sun, Mubai; Miao, Xinyu; Jin, Xin; Xu, Xifei; Su, Ying; Xu, Hongyan; Wang, Jinghui; Niu, Honghong

doi:10.1016/j.bbrc.2022.03.012

Cited by 14 publications

(7 citation statements)

References 22 publications

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“…[21] The activation of the PI3K/AKT signaling pathway could improve insulin sensitivity and alleviate T2DM. [19,22] The impaired PI3K/AKT signaling pathway is strongly related to insulin resistance, [18][19][20] and therefore was suggested as a potential target for treating T2DM. [23][24][25][26] Notably, in the core network of LBW, we also observed a key modulator of this pathway, EGF, which showed the highest stress in the PPI network of LBW.…”

Section: Discussionmentioning

confidence: 99%

“…This pathway is not only a key regulator of early embryonic development but also closely connected with the development of insulin resistance. [18][19][20] Insulin resistance is a disorder of glucose metabolism that plays a crucial role in T2DM. [21] The activation of the PI3K/AKT signaling pathway could improve insulin sensitivity and alleviate T2DM.…”

Section: Discussionmentioning

confidence: 99%

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Network exploration of gene signatures underlying low birth weight induced metabolic alterations

et al. 2022

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Background: This study explored underlying gene signatures of low birth weight (LBW) by analyzing differentially expressed genes (DEGs) between LBW and normal birth weight (NBW) subjects.Methods: Subjects with different birth weight was collected from GEO database. P < .05 and | logFC | ≥ 1.0 were used for screening DEGs. David (2021 Update) was used to perform GO annotation and KEGG signaling pathway enrichment analysis. The protein-protein interaction network of DEGs was constructed using the STRING database, in which hub genes were mined through Cytoscape software.Results: A total of 326 DEGs were identified, including 287 up-regulated genes and 39 down-regulated genes. The GO biological processes enriched by DEGs mainly involved epidermal growth, keratinization and intermediate fibrous tissue. The DEGs were significantly enriched in intracellular insoluble membranes, desmosomes and extracellular space. Their molecular functions mainly focused on structural molecular activity, structural components of epidermis and structural components of cytoskeleton. PI3K/ AKT signaling pathway and tight junction were highlighted as critical pathways enriched by DEGs. Ten hub genes which included KRT14, EGF, DSP, DSG1, KRT16, KRT6A, EPCAM, SPRR1B, PKP1, and PPL were identified from the constructed proteinprotein interaction network. Conclusion:A total of 326 DEGs and 10 hub genes were identified as candidates for metabolic disorders in LBW individuals. Our results indicated PI3K/AKT signaling pathway as an intrauterine adaptive mechanism for LBW individuals. We observed activated PI3K/AKT pathway in LBW individuals, which would promote growth and development at the early stage of life, but adversely introduce extra metabolic stress and thereby potentially induce metabolic disorders in adulthood.Abbreviations: BP = biological process, CC = cellular component, DAVID = database for annotation, visualization and integrated discovery, DEGs = differentially expressed genes, GEO = gene expression omnibus, GO = gene ontology, KEGG = kyoto encyclopedia of genes and genomes, LBW = low birth weight, MF = molecular function, PPI = protein-protein interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Network exploration of gene signatures underlying low birth weight induced metabolic alterations

et al. 2022

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“…After acclimatization for 7 days, the rats were randomly divided into two groups for 8 weeks: ND group fed with standard diet ( n = 8); Model group fed with HFD to established obesity model ( n = 40). The obesity model building standard was as described previously (Sun et al, 2022). Then, Model group rats were randomly grouped into five groups ( n = 8): HFD group, HFD‐O group (Orlistat, 10 mg/kg/bw/day) and three experimental groups of JLCC513 (HFD‐L group, 1.0 × 10 6 CFU ml −1 ; HFD‐M group, 1.0 × 10 8 CFU ml −1 ; HFD‐H group, 1.0 × 10 10 CFU ml −1 ; every time, 0.5 ml/100 g/bw/day) for 8 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…A strain of JLCC513 was screened and identified in our laboratory, and its ability to resist intestinal acid environment and inhibit pathogens was confirmed. In addition, our previous studies have shown that the strain inhibits the development of obesity in rats by regulating blood lipids, inflammation and insulin resistance (Sun et al, 2022). However, the mechanism of B. subtilis natto improving obesity by improving intestinal barrier function is not clear.…”

Section: Introductionmentioning

confidence: 99%

Effects of Bacillus subtilis natto JLCC513 on gut microbiota and intestinal barrier function in obese rats

Sun

Niu

Sun

et al. 2022

Journal of Applied Microbiology

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Aims: This study aimed to investigate the effects of Bacillus subtilis natto JLCC513 (JLCC513) on gut microbiota, inflammation and intestinal barrier function in highfat-diet (HFD) rats. Methods and Results: Sprague-Dawley (SD) rats were fed HFD for 16 weeks, and treated with JLCC513 in 9th week. The oral administration of JLCC513 decreased body weight and reduced the inflammation level in HFD rats. Pathologically, JLCC513 prevented the detachment of ileal villus and increased the villus height in rats. Mechanistically, western blot analysis showed that the protein levels of tight junction (TJ) proteins involved in intestinal barrier function, including zonula occludens-1 (ZO-1), occludin and claudin-1, were increased after JLCC513 treatment. Meanwhile, JLCC513 treatment also decreased the protein levels of toll-like receptor 4 (TLR4), nuclear factor kappa-B (NF-κB) and NOD-like receptor protein 3 (NLRP3), indicating inhibition of the TLR4/NF-κB/NLRP3 pathway. Furthermore, faecal analysis showed that JLCC513 increased the abundance of Lactobacillus and Oscillospira and the ratio of Firmicutes/Bacteroidetes (F/B), and decreased the levels of Blautia and C_Clostridium. Conclusions: JLCC513 alleviated intestinal barrier dysfunction by inhibiting TLR4/ NF-κB/NLRP3 pathway and regulating gut microbiota disorders. Significance and Impact of Study: Our study might provide new treatment strategies for obesity and metabolic diseases.

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“…It has been shown that mixed fermented horse milk with lactic acid bacteria, yeasts, and Monascus is able to regulate blood lipid levels in hyperlipidemic mice 17 . Nattokinase produced during fermentation by B. natto has thrombolytic and lipid‐lowering effects, 18,19 while Monascus is one of the first beneficial fungi applied in food processing and has been used in China for nearly a thousand years. Monascus fermentation can produce a variety of bioactive substances with hypolipidemic effects, such as monacolin K, Monascus pigment, and γ‐aminobutyric acid 20,21 .…”

Section: Introductionmentioning

confidence: 99%

Effects of the polypeptide from peanut meal mixed fermentation on lipid metabolism and intestinal flora of hyperlipidemic mice

Ding

Zhang

et al. 2023

J Sci Food Agric

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BACKGROUND Hyperlipidemia is one of the metabolic disorders posing great threat to human health. Our previous studies have shown that the nutritional properties of peanut meal after fermentation are markedly improved, and can effectively improve hyperlipidemia caused by high‐fat diet in mice. In this study, in order to facilitate the further utilization of peanut meal, the effect of peanut polypeptide (PP) from peanut meal mixed fermentation on lipid metabolism in mice fed with high‐fat diet (HFD) and its possible mechanism were investigated. Fifty male C57BL/6J mice were randomly divided into five groups: normal control group (N), high‐fat model group (M), PP low‐dose group (PL), PP high‐dose group (PH), and atorvastatin positive control group (Y). RESULTS The results show that PP supplementation can effectively reduce the body weight of mice, decrease the serum total cholesterol (TC), triglyceride (TG), low‐density lipoprotein cholesterol (LDL‐C) and leptin levels (P < 0.05), increase the high‐density lipoprotein cholesterol (HDL‐C) levels (P < 0.05), up‐regulate the expression levels of ileal tight junction proteins ZO‐1 and occludin (P < 0.05), reduce the hepatocyte injury and lipid accumulation caused by high‐fat diet and increase the species richness of intestinal flora. CONCLUSION PP can significantly improve hyperlipidemia and regulate intestinal flora disorders caused by hyperlipidemia. The possible mechanism may be related to the reduction of serum leptin levels and up‐regulating the expression levels of the ileal tight junction proteins ZO‐1 and occludin. This study provides evidence for its regulatory role in lipid metabolism and intestinal function, and provides a research basis for the potential nutritional benefits of underutilized food by‐products. © 2023 Society of Chemical Industry.

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Bacillus natto ameliorates obesity by regulating PI3K/AKT pathways in rats

Cited by 14 publications

References 22 publications

Network exploration of gene signatures underlying low birth weight induced metabolic alterations

Network exploration of gene signatures underlying low birth weight induced metabolic alterations

Effects of Bacillus subtilis natto JLCC513 on gut microbiota and intestinal barrier function in obese rats

Effects of the polypeptide from peanut meal mixed fermentation on lipid metabolism and intestinal flora of hyperlipidemic mice

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