Bacillus mycoides Improves Health of Gastrointestinal Tract in Marron (Cherax cainii, Austin 2002)

Abstract

The present study examined the health status of the gastrointestinal tract (GIT) in marron after a two-month feeding trial with a diet supplemented with host origin, Bacillus mycoides. Two groups of marron were fed with either a basal diet or probiotic added at 10 8 cfu/g of feed. Microbial density, microvilli length and number, intestinal epidermis layer morphology and hepatopancreas indices of weight and moisture content were evaluated. Supplementation with B. mycoides in marron feed significantly improved intestinal bacterial density (4,007±121 million cfu/g of GIT) compared to basal diet fed marron (723.7±45.2 million cfu/g of GIT). Microvilli density (per 100 µm 2 ) was also significantly higher (10.50±0.25 µm) compared to 5.71±0.24 in basal diet fed marron. Significantly higher villous length (4.93±0.11 µm) was observed in probiotic fed compared to basal diet fed marron (3.91±0.18 µm).The intestinal epidermis layer of probiotic fed marron showed increased folding and thickness compared to basal diet fed marron. Higher hepatosomatic indices (Hiw) and low moisture content (HM%) in probiotic fed marron indicated efficient functioning of the healthy gut. The present study suggests that supplementing host origin customised probiotic in feed improves gut health as measured by microbial density, microvilli length and number, intestinal epidermis layer and hepatosomatic indices.

Survival and Immunity of Marron Cherax cainii (Austin, 2002) Fed Bacillus mycoides Supplemented Diet under Simulated Transport

“…Before use, the pelleted diet was homogenised with a blender to obtain a desirable pellet size before supplementation with the B. mycoides at 10 8 cfu/g of feed. The density of B. mycoides was based on the density used in other Bacillus species studies [32][33][34][35][36] and from results of our previous studies [18,19]. Supplementation of the probiotic followed established methods [37].…”

“…Our previous studies indicated that Bacillus mycoides, a marron origin customised probiotic, possessed a number of favourable probiotic properties [17], significantly improved gastrointestinal (GIT) health of marron [18] and improved immunity against the pathogenic bacterium Vibrio mimicus [19], a dominant bacterial pathogen of freshwater crayfish in aquaculture [20][21][22].…”

Synbiotic effect ofBacillus mycoidesand organic selenium on immunity and growth of marron,Cherax cainii(Austin, 2002)

“…In the present study, B. mycoides was selected as it was of host origin and possessed a number of probiotic properties (Ambas, Buller et al . ) such as improved marron immunity against V. mimicus (Ambas et al . ) and improved GIT health status (Ambas, Fotedar et al .…”

“…Sel‐plex, an organic form of selenium derived from yeast produced by Alltech, showed beneficial effects in marron when administered at 0.2 g kg − 1 of feed (Nugroho & Fotedar ). Bacillus mycoides, a strain isolated from the gastrointestinal tract of marron, demonstrated favourable probiotic properties such as growth inhibition of Vibrio mimicus and Vibrio cholerae non‐01 (Ambas, Buller & Fotedar ), and improvement in marron health and immunity (Ambas, Suriawan & Fotedar ; Ambas, Fotedar & Buller ). The present study examined the synbiotic effect of the probiotic B. mycoides and the prebiotic OS (Sel‐Plex) on growth, health condition and immunity as tested by total haemocyte count (THC), DHC, bacteraemia, clotting time and glutathione peroxidase ( GP x) enzyme activity of marron.…”

Performance of marron (Cheraxcainii) origin probiotic Bacillus mycoides in earthen commercial marron ponds

“…7,19 Bacillusmycoidesis a predominated bacterium isolated from a number of healthy adult farmed marron GIT that exhibit favourable probiotic properties such as non-pathogenicity to marron, antagonism ability towards common crayfish pathogens (Vibrio mimicus and V. cholerae non-01), exhibition of a diverse enzyme profiles and nonsusceptible to the majority of antibiotics tested 20 improved immunity 21 and the gastrointestinal health status of marron. 22 To date, evaluating probiotic performance in vivo studies is limited to tiger shrimp Penaeusmonodon, 23 shrimp Litopenaeusvannamei 24 and beluga Husohuso. 25 When evaluating probioticsin vivo for its nutritional benefit outcomes in aquatic animals, the probiotic candidates should be added to the diet and its effect evaluated on the growth and/or physiological status of the animals.…”