Bacillus megaterium—from simple soil bacterium to industrial protein production host

Vary, Patricia S.; Biedendieck, Rebekka; Fuerch, Tobias; Meinhardt, Friedhelm; Rohde, Manfred; Deckwer, Wolf‐Dieter; Jahn, Dieter

doi:10.1007/s00253-007-1089-3

Cited by 228 publications

(156 citation statements)

References 90 publications

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“…In this study, it is speculated that the H1 sequence that lined up with Pseudomonas citronellolis (Table 2) may represent a variant of this species, or even a new species of this genus, because the 18 extra nucleotides found within the alignment region were not identified in any of the 16S rRNA sequences deposited in GenBank thus far (data not shown). The H2 isolate aligned strongly with an accession of Bacillus megaterium, described as a mostly aerobic spore-forming bacterium, found in a wide variety of ecological niches and capable of growing in an array of carbon sources, which has been used for more than 50 years in the protein-producing industry (Vary et al, 2007). This species has been studied for industrial fermentation based on crude glycerol from biodiesel production as the substrate (Posada et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

Ta³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. This could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. We explored an Atlantic Rainforest soil sample to search for crude glycerol-degrading microorganisms. Microcosms of this soil were established containing minimal medium + 8% crude glycerol (w/w); the biological activity was measured by respirometry. High CO 2 levels were found in some of the crude glycerol microcosms, suggesting the activity of microorganisms capable of degrading this residue. In an attempt to isolate and cultivate these microorganisms in vitro, aliquots of the soil suspension were plated on minimal medium containing 10% crude glycerol (v/v). Out of 19 morphologically distinct isolates, 12 bacteria and 6 yeasts were identified by PCR from universal primers 16S and 26S rDNA, respectively. Optical density readings revealed growth differences among cultures. Microbial crude-glycerol degraders from rainforest soil Two yeasts and three bacteria with distinct growth profiles stood out and appeared to have potential for liquid fermentation of crude glycerol. The yeasts adapted rapidly, but produced relatively little biomass. Opposite tendencies were found in the bacteria. Amplicon sequencing placed the bacterial isolates as close to Staphylococcus arlettae, Pseudomonas citronellolis, and Bacillus megaterium, and the yeasts to Trichosporon moniliiforme and Meyerozyma guilliermondii. We concluded that these species have potential for use in crude glycerol bioreactors and for bioremediation processes.

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Section: Discussionmentioning

confidence: 99%

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

Ta³

et al. 2013

Genet. Mol. Res.

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“…There are a number of possible reasons that contribute to this low expression. B. megaterium is an ideal host largely because it lacks alkaline proteases produced by B. subtilis that can degrade heterologous protein [7]. However, strains of B. subtilis that are deficient in numerous proteases have been developed, which make them a more improved host for heterologous protein expression [13].…”

Section: Discussionmentioning

confidence: 99%

“…Shuttle vectors have become available that allow for cloning in E. coli and expression in B. megaterium. This bacterium has been shown to produce many industrial and commercially important products including antimicrobial synthetic proteins and vitamins [7]. However, protein expression is often higher in B. megaterium than in B. subtilis, and B. megaterium lacks alkaline proteases found in B. subtilis and also exhibits good plasmid stability [7].…”

Section: Introductionmentioning

confidence: 99%

Expression of Low Endotoxin 3-O-Sulfotransferase in Bacillus subtilis and Bacillus megaterium

Wang

Englaender

et al. 2013

Appl Biochem Biotechnol

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A key enzyme for the biosynthesis and bioengineering of heparin, 3-O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium -fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources.

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“…Further, it is used for the production of pyruvate, vitamin B12, drugs with fungicidal and antiviral properties, etc. (Vary et al, 2007). It produces enzymes for modifying corticosteroids as well as several amino acid dehydrogenases.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Bacillus megaterium Bacteriophages via AFLP Technique

Elmaghraby¹,

Carimi²,

Sharaf³

et al. 2015

Current Research in Bacteriology

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Ten bacteriophages specific for Bacillus megaterium were isolated from a clay loam soil sample collected from the Experimental Farm of Faculty of Agriculture, Minia University, Minia, Egypt. Four out of ten isolates were inactivated after exposure to 80°C for 10 min and three isolates were inactivated at 78°C for 10 min. Whereas, the other three phage isolates were inactivated at 82°C for 10 min. The isolated phages were found to be tolerant to wide range of pH 5-9. The longevity in vitro varied between the phage isolates. The highest longevity in vitro was recorded for four phage isolates (192 h). Electron micrographs of the isolated phages indicated that all phage isolates were of the head and tail types. Two different host specificities were observed for the ten phage isolates (two different populations). Six phage isolates (population 1) were found to be infectious to B. megaterium among the four species tested (i.e., B. megaterium, B. circulans, B. polymexa and B. subtilis). Whereas, the rest of the phage isolates (population 2) were found to be infectious to B. megaterium and B. subtilis. The dendrogram separated the 10 phage isolates into two main clusters (two populations) and then each cluster was separated into two sub clusters. Isolates that belonged to the same host range were grouped together. The percentage of variation was 9% among populations and 91% within populations. The five most remarkable isolates were submitted to the bacillus database and named BMC1, BMC2, BMC3, BMC4 and BMC5.

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Bacillus megaterium—from simple soil bacterium to industrial protein production host

Cited by 228 publications

References 90 publications

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

Expression of Low Endotoxin 3-O-Sulfotransferase in Bacillus subtilis and Bacillus megaterium

Isolation and Identification of Bacillus megaterium Bacteriophages via AFLP Technique

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