Bacillus hisashii sp. nov., isolated from the caeca of gnotobiotic mice fed with thermophile-fermented compost

Abstract

A taxonomic study was performed on 15 bacterial isolates from the caeca of gnotobiotic mice that had been fed with thermophile-fermented compost. The 15 isolates were thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming bacteria, and were most closely related to Bacillus thermoamylovorans CNCM I-1378 T isolate. All 15 isolates grew at 25-60 8C (optimum 50 8C), pH 6-8 (optimum pH 7) and were capable of growing on a medium containing 6 % (w/v) NaCl (optimum 0.5 %). The 15 isolates could be distinguished from B. thermoamylovorans LMG 18084 T because they showed Tween 80 hydrolysis activity and did not produce acid from melibiose. The major fatty acids were anteiso-C 15 : 0 , C 16 : 0 , iso-C 15 : 0 , iso-C 14 : 0 and iso-C 16 : 0 . The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and several unidentified phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The menaquinone was MK-7.

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

“…The description of this species is the same as provided by Nishida et al [85] for Bacillus hisashii.…”

“…The type species is Caldibacillus debilis [64,84]. The description of this species is the same as provided by Nishida et al [85] for Bacillus hisashii.…”

Meta‐fermentation system with a mixed culture for the production of optically pure l‐lactic acid can be reconstructed using the minimum isolates with a simplified pH control strategy

“…To construct the meta‐fermentation system for l ‐LA production, the isolated strains of W. coagulans (MN‐07, MC‐02, MN‐09, 24C‐1, NYC‐4, NA50‐3, and CPh‐14) and C. thermoamylovorans (MC‐07, OM55‐6, MC‐04, CPh‐4, and MN‐04) [ 18 ] were considered based on DGGE analysis. [ 7 ] Additionally, two strains (N‐11 and N‐21) of C. hisashii , which exhibits 99.4% pairwise sequence similarity with C. thermoamylovorans , [ 17 ] were investigated. C. hisashii and C. thermoamylovorans are not distinguishable based on 16S rRNA gene fragments in both DGGE and MiSeq 16S amplicon analyses.…”

“…Because the cell sizes are reported to be similar among W. coagulans , C. thermoamylovorans , and C. hisashii , [ 17,21,22 ] the cell concentrations would be proportional to the OD values of each bacterial culture. The pre‐cultured of each strain were individually performed for 20 h, and then, the respective pre‐cultured broths (OD 610 = 1.0) of 3 isolates were inoculated directly into 400 ml of MKR medium in a 1000‐ml jar fermenter equipped with a pH controller (DJ‐1023P, Able, Tokyo, Japan).…”

“…[ 5 ] Seven major species ( Corynebacterium sphenisci , Bacillus thermocloacae , C. thermoamylovorans , Bacillus smithii , Bacillus humi , Caldibacillus hisashii (synonym: Bacillus hisashii ), and W. coagulans ) have been successfully isolated from the meta‐LA fermentation system. [ 7,17,18 ] It is reported that the performances of lactic acid production would be different among bacteria at strain level in pure culture systems. [ 16 ] Thus, to further develop the meta‐fermentation system, the fermentation process must be investigated by individually tracing the related strains.…”