Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern

Pappa, A.; Sánchez‐Porro, Cristina; Lazoura, P.; Kallimanis, A.; Perisynakis, Angelos; Ventosa, António; Drainas, Constantin; Koukkou, Anna‐Irini

doi:10.1099/ijs.0.014233-0

Cited by 25 publications

(11 citation statements)

References 26 publications

(16 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They are sporogenic and produce endospores, which are resistant to different physical and chemical agents. They are common inhabitants of terrestrial and fresh water habitats and excel in extreme conditions including hypersaline water, hydrothermal vents and salt pans (Rüger & Richter, 1979;Arahal et al, 1999;Caccamo et al, 2000;Pappa et al, 2010;Zhang et al, 2010). They also occur in the stratosphere (Shivaji et al, 2006).…”

mentioning

confidence: 99%

Bacillus luteus sp. nov., isolated from soil

Yadav

Sasikala

Ramana

2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Two bacterial strains (JC167 T and JC168) were isolated from a soil sample collected fromMandpam, Tamilnadu, India. Colonies of both strains were orange and cells Gram-stain-positive. Cells were small rods, and formed terminal endospores of ellipsoidal to oval shape. Both strains were positive for catalase, oxidase and hydrolysis of starch/gelatin, and negative for chitin hydrolysis, H 2 S production, indole production and nitrate reduction activity. Major fatty acids of both strains (.5 %) were anteiso-C 15 : 0 , iso-C 16 : 0 , iso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 14 : 0 and C 16 : 0 with minor (,5 but .1 %) amounts of iso-C 17 : 0 , anteiso-C 17 : 0 B/iso-C 17 : 0 I and C 16 : 1 v11c. Diphosphatydilglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell wall amino acids were L-alanine, D-alanine, D-glutamic acid and meso-diaminopimelic acid. b-Carotene and five unidentified carotenoids were present in both strains. Mean genomic DNA G+C content was 53.4±1 mol% and the two strains were closely related (mean DNA-DNA hybridization .90 %). 16S rRNA gene sequence comparisons of both strains indicated that they represent species of the genus Bacillus within the family Bacillaceae of the phylum Firmicutes. Both strains had a sequence similarity of 97.6 % with Bacillus saliphilus 6AG T and ,96.8 % with other members of the genus Bacillus. Sequence similarity between strain JC167 T and 168 was 100 %. Strain JC167 T showed 25.8±1 % reassociation (based on DNA-DNA hybridization) with B. saliphilus DSM 15402 T (56AG T ).Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC167 T as a representative of a novel species of the genus

show abstract

mentioning

confidence: 99%

Bacillus luteus sp. nov., isolated from soil

Yadav

Sasikala

Ramana

2014

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

“…Finally, in the last pond (called crystallizer), common salt is precipitated [4]. Many studies have focused on the isolation of bacteria harbored in hypersaline environments, identifying the following major groups: Bacteroidetes [12], Firmicutes [13][14][15], ϒ-Proteobacteria [13,[16][17], and ϒ-Proteobacteria being the most abundant.…”

Section: Solar Salternsmentioning

confidence: 99%

“…Mei et al [64] describe the physiology of a Natrinema sp. strain J7-2, an extremely halophilic archaea isolated from a salt mine in China, under salt stress conditions (15,25,and 30% NaCl). This strain showed the highest growth rate at 25-30% of NaCl, while at 15% cells were more fragile.…”

Section: Synthesis Of Intracellular Compoundsmentioning

confidence: 99%

Kinetics of Halophilic Enzymes

Cira‐Chávez¹,

Guevara-Luna²,

YadiraSoto-Padilla³

et al. 2019

Kinetics of Enzymatic Synthesis

View full text Add to dashboard Cite

Hypersaline environments are those with salt concentrations 9-10 times higher (30-35% of NaCl) than sea water (3.5% of NaCl). At high concentrations of soluble salts, cytoplasm-mainly of bacteria and archaea-is exposed to high ionic strength and achieves osmotic equilibrium by maintaining a cytoplasmic salt concentration similar to that of the surrounding media. Halophilic enzymes are extremozymes produced by halophilic microorganisms; they have similar characteristics to regular enzymes but different properties, mainly structural. Among these properties is a high requirement of salt for biological functions. Furthermore, the discovery of enzymes capable of degrading biopolymers offer a new perspective in the treatment of residues from oil deposits, under typically high conditions of salt and temperature, while giving valuable information on heterotrophic processes in saline environments.

show abstract

“…Bacillus species have been isolated from various habitats, including air (Shivaji et al 2006), ancient tombs (Gatson et al 2006), brown alga (Ivanova et al 2004), desert sands , forest soils (Chen et al 2011), freshwater (Baik et al 2010), ginseng root (Qiu et al 2009), hot springs (Nazina et al 2004), marine sediments (You et al 2013;Zhu et al 2014), sea water (Yoon et al 2003), sandy soil (Lee et al 2008) and a soda lake (Reddy et al 2015). Many can be found in saline habitats (Amoozegar et al 2009;Arahal et al 1999;Lee et al 2006;Lim et al 2006;Pappa et al 2010;Shi et al 2011;Xue et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China

Zhang

Zhang³

et al. 2015

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

A novel aerobic bacterium, KC1(T), was isolated from a salt mine in Kuche county, Xinjiang province, China. Cells were observed to be Gram-positive, rod-shaped, endospore-forming and motile with flagella. Strain KC1(T) was found to grow at 25-45 °C (optimum 37 °C), pH 6.5-9.0 (optimum 8.0) and NaCl 0-10 % (v/v) (optimum 4 %). The major fatty acids were identified as anteiso-C15:0 and anteiso-C17:0. Menaquinone-7 (MK-7) was found to be the predominant isoprenoid quinone. The cell-wall diamino acid was found to be meso-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol and a glycolipid. The 16S rRNA gene sequence of strain KC1(T) showed low similarity (<96 %) to other validly named species. The phylogenetic trees showed that strain KC1(T) is closely related to Bacillus azotoformans DSM 1046(T) and Bacillus methanolicus DSM 16454(T). Both these type strains showed 95.4 % 16S rRNA gene sequence similarity to strain KC1(T). The DNA G+C content of strain KC1(T) was determined to be 39.0 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain KC1(T) is considered to represent a novel species of the genus Bacillus, for which the name Bacillus salitolerans sp. nov. is proposed. The type strain is KC1(T) (=JCM 19760(T) = CGMCC 1.12810(T)).

show abstract

Bacillus halochares sp. nov., a halophilic bacterium isolated from a solar saltern

Cited by 25 publications

References 26 publications

Bacillus luteus sp. nov., isolated from soil

Bacillus luteus sp. nov., isolated from soil

Kinetics of Halophilic Enzymes

Bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in Xinjiang province, China

Contact Info

Product

Resources

About