Bacillus fengqiuensis sp. nov., isolated from a typical sandy loam soil under long-term fertilization

Abstract

A Gram-staining-positive, endospore-forming, moderately alkaliphilic bacterium, strain NPK15T, was isolated from a typical sandy loam soil under long-term NPK fertilization in northern China and was subjected to a polyphasic taxonomic study. The diamino acid of the cell-wall peptidoglycan of strain NPK15T was found to be meso-diaminopimelic acid and the cell-wall sugars were xylose, glucose and traces of mannose. The only respiratory quinone found in strain NPK15T was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω6c/C16 : 1ω7c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis of the strain based on its 16S rRNA gene sequence showed that it was related most closely to ‘ Bacillus thaonhiensis ’ KACC 17216 (99.59 %), B. songklensis KCTC 13881T (99.52 %) and B. abyssalis CCTCC AB 2012074T (99.00 %). DNA–DNA hybridization results indicated that the strain was distinct from other species of the genus Bacillus , the degree of relatedness being 35.4 % with B. abyssalis CCTCC AB 2012074T, 39.7 % with B. songklensis KCTC 13881T and 51.2 % with ‘ B. thaonhiensis ’ KACC 17216. The DNA G+C content of strain NPK15T was 45.5 mol%. Phenotypic, chemotaxonomic and molecular analyses identified strain NPK15T as a member of a novel species of the genus Bacillus , for which the name Bacillus fengqiuensis sp. nov. is proposed. The type strain is NPK15T ( = DSM 26745T = CCTCC AB 2013156T).

Role of fatty acids in Bacillus environmental adaptation

“…The Bacillus genus is a large genus in which the number of novel strains characterized is increasing ( Choi and Cha, 2014 ; Kosowski et al, 2014 ; Zhao et al, 2014 ). The FA profiles of Bacillus genus strains are used, in addition to various other molecular data, as a biomarker for taxonomy.…”

Lysinibacillus alkaliphilus sp. nov., an extremely alkaliphilic bacterium, and emended description of genus Lysinibacillus

“…Strain OMN17 T was isolated from a typical sandy loam soil that was part of the State Experimental Station for Agro-Ecology, Fengqiu country (35° 00′ N 114° 24′ E), Henan province, China. The soil had been described previously by the authors (Zhao et al , 2014). A standard dilution-plating technique was used to isolate the strain on nutrient agar (5 g peptone, 3 g meat extract, 1 l distilled water; pH 7.2–7.4) at 30 °C for 2 days.…”

Draft Genome Sequence of Bacillus fengqiuensis FJAT-14578, Isolated from a Soil Sample in China

“…JX262265.1), a Gram-positive endospore-forming aerobic bacterium, was isolated from a soil sample in China. Recently, this species was first found and reported by Zhao et al (1) and was isolated from a typical sandy loam soil under long-term NPK fertilization in northern China. One genome assembly for the strain B .…”