Bacillus endolithicus sp. nov., isolated from pebbles

Abstract

Strain JC267T was isolated from pebbles collected from Pingleshwar beach, Gujarat, India.Cells are Gram-stain-positive, facultatively anaerobic, non-motile rods forming sub-terminal endospores in swollen ellipsoidal to oval sporangia. Strain JC267 T contains anteiso-C 15 : 0 , iso-C 15 : 0 , iso-C 14 : 0 , iso-C 16 : 0, C 16 : 0 and anteiso-C 17 : 0 as major (.5 %) cellular fatty acids. Polar lipids include phosphatidylglycerol, phospholipids (PL1-3), glycolipids (GL1-2) and an unidentified lipid. Cell-wall amino acids are composed of diagnostic mesodiaminopimelic acid, DL-alanine and a small amount of D-glutamic acid. The genomic DNA G+C content of strain JC267 T is 45.5 mol%. The 16S rRNA gene sequence of strain JC267T showed highest sequence similarities of ,98.41 % with all species of the genus Bacillus when subjected to EzTaxon-e BLAST analysis. The reassociation values based on DNA-DNA hybridization of strain JC267 T with Bacillus halosaccharovorans IBRC-M 10095 T and Bacillus niabensis JCM 16399 T were 26¡1 % and 34¡3 %, respectively. Based on taxonomic data obtained using a polyphasic approach, strain JC267 T represents a novel species of the genus

Genome Sequences of Metabacillus sp. Strain B2-18, Isolated from Human Skin, and Metabacillus endolithicus KCTC 33579 T

“…In this study, we report the whole-genome sequences of Metabacillus sp. strain B2-18 and Metabacillus endolithicus KCTC 33579 T ( 1 ) to confirm the phylogenetic relationship between the two strains. A swab sample from the forehead skin of a female college student was directly streaked on minimal salts basal (MSB) agar ( 2 ) containing 0.5% yeast extract (MSBY medium).…”

Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel

“…At the time of writing (February 2022), the genus Metabacillus comprised 16 species with valid names (https://lpsn.dsmz.de/genus/metabacillus). Members of the genus Metabacillus have been isolated from a wide range of environments such as oysters [2], pebbles [3], faeces [4], blood [5], salt lake [6, 7], sediment [8–10] and wastewater [11]. Cells of the genus Metabacillus are Gram-stain-positive or Gram-stain-variable, aerobic, rod-shaped, motile or non-motile and grow at 4–45 °C.…”

Genomic and Physiological Characterization of Metabacillus flavus sp. nov., a Novel Carotenoid-Producing Bacilli Isolated from Korean Marine Mud

“…All previously reported species of the genus Metabacillus are described as rod-shaped, Gram-stain-positive or Gram variable, and grow aerobically [ 1 ]. Recently, 14 species ( M. fastidiosus , M. indicus , M. herbersteinensis , M. litoralis , M. idriensis , M. niabensis , M. galliciensis , M. halosaccharovorans , M. crassostreae , M. endolithicus , M. malikii , M. mangrovi , M. iocasae , and M. lacus ) have been transferred from the genus Bacillus to the genus Metabacillus [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]. Only two species, M. sediminilitoris and M. elymi isolated from tidal sediments and plants native to the Dokdo Island were initially proposed as novel species of the genus Metabacillus [ 16 , 17 ].…”