Bacillus cereus sensu lato biofilm formation and its ecological importance

Yang, Lin; Briandet, Romain; Kovács, Ákos T.

doi:10.1016/j.bioflm.2022.100070

Cited by 31 publications

(23 citation statements)

References 110 publications

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“…In B. thuringiensis , the TasA-orthologue CalY has been identified as a cell-surface adhesion and described as a major virulence factor and biofilm matrix protein [51]. SpoVG is a regulator of both sporulation and biofilm formation in B. cereus 0-9 that activates the levels of Spo0A and, thus, the fate of B. cereus cells: biofilm or sporulation [52, 53]. We speculate that the lack of expression of TasA and the biofilm regulatory protein SpoVG could contribute to the differences observed between BT CFU counts and total biofilm biomass for the variant phenotype (CV).…”

Section: Discussionmentioning

confidence: 99%

Evolution of genotypic and phenotypic diversity in multispecies biofilms

Amador,

Moscovitz,

Maccario

et al. 2023

Preprint

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Interactions occurring in microbial communities can affect the fitness and adaptability of their individual members when facing changing environmental conditions. This study investigated the impact of interspecies interactions in selectingBacillus thuringiensisvariants emerging in biofilm and planktonic environments. During evolution experiments, aB. thuringiensisdistinct phenotypic variant ofB. thuringiensisfrequently occurred, despite the presence of other species or culture setup. Remarkably, selection of this variant was significantly favored over its ancestor in biofilm settings and when coexisting with other species co-isolated from a wastewater facility, namelyPseudomonas defluviiandPseudomonas brenneri. Interestingly, the evolved phenotype did not show higher biofilm productivity than its ancestor under any condition, while it was indeed reduced in mixed-species biofilms. Such observation aligned with the reduced abundance of matrix proteins TasA and SpoVG, which are major biofilm matrix components inBacillusspecies, as revealed by matrix proteomics analysis. Furthermore, the variant showed shorter generation time and a lack of sporulation compared to its ancestor, consistent with mutations in key genes for regulating sporulation. Our results indicate that interspecies interactions within biofilms promoteB. thuringiensisdiversification and alter traits such as biofilm matrix production. Despite sporulation being a survival mechanism, this study provides evidence that sporulation does not confer a fitness advantage inin vitrobiofilm environments, even within mixed cultures.

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Section: Discussionmentioning

confidence: 99%

Evolution of genotypic and phenotypic diversity in multispecies biofilms

Amador,

Moscovitz,

Maccario

et al. 2023

Preprint

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“…Similarly, the biofilm-associated genes calY , tasA and the eps2 locus [33, 34], were up-regulated compared to exponentially growing cells. These data suggest that the late-infection stage bacteria form a biofilm which might confer them an advantage for long-term survival considering the resistance properties of these structures [35]. The most represented categories among the up-regulated transcripts in 7 dpi bacteria compared to in vitro -grown cells are the oxidative stress response, DNA recombination, replication and repair in addition to iron homeostasis.…”

Section: Discussionmentioning

confidence: 99%

A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection

Toukabri

Lereclus

Slamti

2022

Preprint

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The formation of endospores has been considered as the unique mode of survival and transmission of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial form. However, the persistence of non-sporulated bacteria (Spo-) was reported during infection in Bacillus thuringiensis, an entomopathogenic sporulating Gram-positive bacterium. In this study, we investigated the behavior of a bacterial population in the late stages of an infection as well as the characteristics of the Spo- bacteria in the B. thuringiensis/Galleria mellonella infection model. Using fluorescent reporters coupled to flow cytometry as well as molecular markers, we demonstrated that the Spo- cells constitute about half of the population two weeks post-infection (pi) and that these bacteria present vitality signs. However, a protein synthesis and a growth recovery assay indicated that they are in a metabolically slowed-down state. Interestingly, they were extremely resistant to the cadaver environment which proved deadly for in vitro-grown vegetative cells and, strikingly, did not support spore germination. A transcriptomic analysis of this subpopulation at 7 days pi revealed a signature profile of this state. The expression analysis of individual genes at the cell level suggests that iron homeostasis is important at all stages of the infection, whereas the oxidative stress response seems of particular importance as the survival time increases. Altogether, these data show that non-sporulated bacteria are able to survive for a prolonged period of time and indicate that they engage in a profound adaptation process that leads to their persistence in the host cadaver.

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“…Typically, microbes related to the food industry such as B. cereus , Enterohemorrhagic E. coli , L. monocytogenes , and S. enterica , are predominantly present in the mixed biofilm state [ 7 ]. Moreover, B. cereus forms endospores that can withstand pasteurization conditions and make robust biofilms [ 29 ]. The enterotoxin (hemolysin) of B. cereus causes various illnesses, namely diarrhea and abdominal pain [ 7 ].…”

Section: Significance Of Pathogenic Bacteria and Their Biofilms In Th...mentioning

confidence: 99%

Exploring Possible Ways to Enhance the Potential and Use of Natural Products through Nanotechnology in the Battle against Biofilms of Foodborne Bacterial Pathogens

Kannappan

Prasath²,

Sathya

et al. 2023

Pathogens

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Biofilms enable pathogenic bacteria to survive in unfavorable environments. As biofilm-forming pathogens can cause rapid food spoilage and recurrent infections in humans, especially their presence in the food industry is problematic. Using chemical disinfectants in the food industry to prevent biofilm formation raises serious health concerns. Further, the ability of biofilm-forming bacterial pathogens to tolerate disinfection procedures questions the traditional treatment methods. Thus, there is a dire need for alternative treatment options targeting bacterial pathogens, especially biofilms. As clean-label products without carcinogenic and hazardous potential, natural compounds with growth and biofilm-inhibiting and biofilm-eradicating potentials have gained popularity as natural preservatives in the food industry. However, the use of these natural preservatives in the food industry is restricted by their poor availability, stability during food processing and storage. Also there is a lack of standardization, and unattractive organoleptic qualities. Nanotechnology is one way to get around these limitations and as well as the use of underutilized bioactives. The use of nanotechnology has several advantages including traversing the biofilm matrix, targeted drug delivery, controlled release, and enhanced bioavailability, bioactivity, and stability. The nanoparticles used in fabricating or encapsulating natural products are considered as an appealing antibiofilm strategy since the nanoparticles enhance the activity of the natural products against biofilms of foodborne bacterial pathogens. Hence, this literature review is intended to provide a comprehensive analysis of the current methods in nanotechnology used for natural products delivery (biofabrication, encapsulation, and nanoemulsion) and also discuss the different promising strategies employed in the recent and past to enhance the inhibition and eradication of foodborne bacterial biofilms.

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Bacillus cereus sensu lato biofilm formation and its ecological importance

Cited by 31 publications

References 110 publications

Evolution of genotypic and phenotypic diversity in multispecies biofilms

Evolution of genotypic and phenotypic diversity in multispecies biofilms

A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection

Exploring Possible Ways to Enhance the Potential and Use of Natural Products through Nanotechnology in the Battle against Biofilms of Foodborne Bacterial Pathogens

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