Bacillus cereus AR156-Induced Resistance to Colletotrichum acutatum Is Associated with Priming of Defense Responses in Loquat Fruit

Abstract

The effectiveness of a biocontrol agent Bacillus cereus AR156 for control of anthracnose rot caused by Colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. Treatment of fruit with B. cereus AR156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. The treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxidase and polyphenoloxidase, and promoted accumulation of H2O2. Total phenolic content and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity were also increased by treatment. Transcripts of three defense-related genes were enhanced only in fruit undergoing both B. cereus AR156 treatment and C. acutatum inoculation compared with those receiving either intervention alone. These results suggest that the disease resistance against C. acutatum in loquat fruit is enhanced by B. cereus AR156 and that the induced resistance is associated with induction and priming of defense responses in the fruit.

Compost Amendments Based on Vinegar Residue Promote Tomato Growth and Suppress Bacterial Wilt Caused by Ralstonia Solanacearum

“…•− and H 2 O 2 production (Figure 4), which resulted in higher EL and MDA contents ( Figure 5); similar results were obtained in other studies [31,32]. The oxidative stress induced by biotic stress was alleviated by VRS (Figures 4 and 5).…”

Induction of defence-related enzymes in tomato (Solanum lycopersicum) plants treated with Bacillus subtilis CBR05 against Xanthomonas campestris pv. vesicatoria

“…The progress in pest management strategies using endophytes is an emerging area in plant protection to rationalize the damage caused by plant pathogens in economically important crops. Members of genus Bacillus are considered as potential biological control agents due to their broad-spectrum antagonistic activity against fungi and bacteria, high spore production capacity and resistance to unfavorable conditions (Wulff et al, 2002;Li et al, 2008;Raddadi et al, 2009;Wang et al, 2014;Ferraz et al, 2015;Fousia et al, 2015). In this work, we focused on a Bacillus subtilis CBR05 strain isolated from rice plants that had potent biocontrol activities against the phytopathogenic fungi, F. oxysporum and R. solani (Ji et al, 2014).…”

Bacterial antagonists and hexanal-induced systemic resistance of mango fruits against Lasiodiplodia theobromae causing stem-end rot

“…Combining different biocontrol agents can contribute to better control of pathogens (Spadaro & Gullino 2005). Recent study imply that combined application of bacterial antagonists like P. fluorescens, B. subtilis with hexanal drastically reduced the pathogen population in vitro and strengthen the cell wall structures resulting in restriction of pathogen infection (Ahemad & Kibret 2014;Wang et al 2014). The recent approach is to design the non-chemical strategy by combining the positively compatible approaches to achieve effective disease control.…”

“…Therefore, use of compatible bacterial antagonist as consortia would emphasize more effective against postharvest diseases (Sangeetha et al 2010). Induction of defense enzymes has been reported against several postharvest pathogens by bioinoculants (Peeran et al 2014;Wang et al 2014;Guo et al 2015). With this background the present study was taken up with the objective to unravel the induction of various defense-related genes encoding proteins incriminated in strengthening of host cell walls due to effective combination of lipoxygenase compound hexanal and bacterial biocontrol agent's treatments in response to infection by L. theobromae.…”