Bacillus amyloliquefaciens Probiotics Mix Supplementation in a Broiler Leaky Gut Model

Horyanto, Darwin; Bajagai, Yadav S.; Kayal, Advait; von Hellens, Juhani; Chen, Xiaojing; Van, Thi Thu Hao; Radovanović, Anita; Stanley, Dragana

doi:10.3390/microorganisms12020419

Cited by 3 publications

(2 citation statements)

References 83 publications

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“…The broiler performance data, including their body weight (BW), average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR) before and after the DEX periods, are provided in Supplementary Figures S1 and S2 [ 31 ]. There was no significant difference in the broiler BW, ADG, ADFI or FCRs between the control and probiotic groups before DEX supplementation, except on day 21 ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

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The Association between Broiler Litter Microbiota and the Supplementation of Bacillus Probiotics in a Leaky Gut Model

Horyanto,

Bajagai,

von Hellens

et al. 2024

Animals

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Probiotics provided from hatch have a major influence on microbiota development, and together with environmental and bedding microbiota, shape the microbial community of the litter. We investigated the influence of probiotic supplementation and a leaky gut challenge induced using dexamethasone (DEX) on the litter microbial community and litter parameters. The probiotic product was a mix of three Bacillus amyloliquefaciens strains. The litter microbiota were compared to the microbial communities from other gut sections. The litter samples had higher microbial diversity compared to the caecum, gizzard, jejunum, and jejunal mucosa. The high similarity between the litter phylum-level microbiota and gizzard microbiota detected in our study could be a consequence of ingested feed and litter passing through the gizzard. Moreover, the litter microbial community is fundamentally distinct from the intestinal microbiota, as evidenced by the number of genera present in the litter but absent from all the intestinal sections and vice versa. Furthermore, LEfSe analysis identified distinct microbial taxa across different groups, with specific genera associated with different treatments. In terms of litter quality, the birds in the DEX groups had a significantly higher moisture content, indicating successful leaky gut challenge, while probiotic supplementation did not significantly affect the moisture levels. These findings provide comprehensive insights into the distinct microbiota characteristics of litter.

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Section: Resultsmentioning

confidence: 99%

“…In addition to the litter, the microbiota samples collected in this study included gizzard, caecum, jejunum and jejunal swabs. The microbial communities of each gut section and animal performance data have been published separately by Horyanto et al [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

The Association between Broiler Litter Microbiota and the Supplementation of Bacillus Probiotics in a Leaky Gut Model

Horyanto,

Bajagai,

von Hellens

et al. 2024

Animals

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Mechanisms of intestinal injury in polychaete Perinereis aibuhitensis caused by low-concentration fluorene pollution: Microbiome and metabonomic analyses

Teng,

Yang,

et al. 2024

Journal of Hazardous Materials

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Intestinal Microbiome Profiles in Broiler Chickens Raised with Different Probiotic Strains

da Silva,

Almeida,

Borsanelli

et al. 2024

Microorganisms

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The composition of the intestinal microbiota can influence the metabolism and overall functioning of avian organisms. Therefore, the objective of this study was to evaluate the effect of three different probiotics and an antibiotic on the microbiomes of 1.400 male Cobb® broiler raised for 42 days. The experiment was conducted with the following treatments: positive control diet (basal diet + antibiotic); negative control diet (basal diet without antibiotic and without probiotic); basal diet + Normal Avian Gut Flora (NAGF); basal diet + multiple colonizing strain probiotics (MCSPs); and basal diet + non-colonizing single strain probiotics (NCSSPs). The antibiotic (enramycin—antibiotic growth promoter) and probiotics were administered orally during all experiment (1 to 42 days), mixed with broiler feed. To determine the composition of the microbiota, five samples of ileal digesta were collected from 42-day-old chickens of each experimental group. The alpha and beta diversity of the ileal microbiota showed differences between the groups. MCSPs presented greater richness and uniformity compared to the positive control, negative control, and NCSSPs treatments, while the negative control exhibited greater homogeneity among samples than NCSSPs. MCSPs also showed a higher abundance of the genus Enterococcus. There were differences between the groups for low-abundance taxa (<0.5%), with NAGF showing higher levels of Delftia, Brevibacterium, and Bulleidia. In contrast, NCSSPs had a higher abundance of Ochrobactrum, Rhodoplanes, and Nitrospira. It was concluded that the treatments analyzed in this study induced modulations in the ileal microbiota of the chickens examined.

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Bacillus amyloliquefaciens Probiotics Mix Supplementation in a Broiler Leaky Gut Model

Cited by 3 publications

References 83 publications

The Association between Broiler Litter Microbiota and the Supplementation of Bacillus Probiotics in a Leaky Gut Model

The Association between Broiler Litter Microbiota and the Supplementation of Bacillus Probiotics in a Leaky Gut Model

Mechanisms of intestinal injury in polychaete Perinereis aibuhitensis caused by low-concentration fluorene pollution: Microbiome and metabonomic analyses

Intestinal Microbiome Profiles in Broiler Chickens Raised with Different Probiotic Strains

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