Bacillus amyloliquefaciens alters the diversity of volatile and non-volatile metabolites and induces the expression of defence genes for the management of Botrytis leaf blight of Lilium under protected conditions

Nakkeeran, S.; Priyanka, R.; Rajamanickam, Subapriya; Sivakumar, U.

doi:10.1007/s42161-020-00602-6

Cited by 14 publications

(11 citation statements)

References 42 publications

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“…In this sense, B. amyloliquefaciens VB 7 produces extra VOCs, such as oxirane, 3,5-octadiyne, and formic acid, when it is co-cultured with B. cinerea biomass. This can be related to the antifungal activity shown both in vitro and in planta, which inhibited 46% of fungi [98].…”

Section: Synthesis Of Volatile Organic Compoundsmentioning

confidence: 89%

“…Surfactin is another LP with broad-spectrum antimicrobial activity [97]. However, lipoproteins are not the only antifungal metabolites produced by Bacillus; there are plenty of others, such as phthalic acid, hept-3-yl isobutyl ester, and propanoic acid, 2-hydroxy-, and methyl ester, produced by B. amyloliquefaciens VB7, which showed inhibition rates of up to 46% against B. cinerea [98]. On the other hand, Pseudomonas is also a genus that may control B. cinerea efficiently, since its members produce a wide range of metabolites [38], including 2,4-diacetylphloroglucinol (DAPG), pyrrolnitrin, and phenazine [2].…”

Section: Antimicrobial Moleculesmentioning

confidence: 99%

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Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Roca-Couso

Flores-Félix

Rivas

2021

JoF

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Botrytis cinerea is a phytopathogenic fungus responsible for economic losses from USD 10 to 100 billion worldwide. It affects more than 1400 plant species, thus becoming one of the main threats to the agriculture systems. The application of fungicides has for years been an efficient way to control this disease. However, fungicides have negative environmental consequences that have changed popular opinion and clarified the need for more sustainable solutions. Biopesticides are products formulated based on microorganisms (bacteria or fungi) with antifungal activity through various mechanisms. This review gathers the most important mechanisms of antifungal activities and the microorganisms that possess them. Among the different modes of action, there are included the production of diffusible molecules, both antimicrobial molecules and siderophores; production of volatile organic compounds; production of hydrolytic enzymes; and other mechanisms, such as the competition and induction of systemic resistance, triggering an interaction at different levels and inhibition based on complex systems for the production of molecules and regulation of crop biology. Such a variety of mechanisms results in a powerful weapon against B. cinerea; some of them have been tested and are already used in the agricultural production with satisfactory results.

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Section: Synthesis Of Volatile Organic Compoundsmentioning

confidence: 89%

Section: Antimicrobial Moleculesmentioning

confidence: 99%

Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Roca-Couso

Flores-Félix

Rivas

2021

JoF

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“…Nakkeeran et al . (2019) and Fernando et al . (2006) reported antifungal activity of antimicrobial peptides, VOCs, and NVOCs from B .…”

Section: Discussionmentioning

confidence: 94%

“…lycopersici. Nakkeeran et al (2019) and Fernando et al (2006) reported antifungal activity of antimicrobial peptides, VOCs, and NVOCs from B. amyloliquefaciens VB7 against Botrytis cinerea, which causes Lilium leaf blight. Furthermore, our study also confirmed that M. odoratimimus produced dihydroacridine.…”

Section: Discussionmentioning

confidence: 99%

Differential bacterial endophytome in Foc‐resistant banana cultivar displays enhanced antagonistic activity against Fusarium oxysporum f.sp. cubense (Foc)

Saravanan

Nakkeeran

Nallusamy

et al. 2021

Environmental Microbiology

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Diverse endophytes with multiple functions exist in different banana cultivars. However, the diversity of cultivable bacterial endophytome that contributes to antifungal activity against Fusarium oxysporum f.sp. cubense (Foc) in resistant and susceptible banana cultivars is mostly unknown. In the present study, we isolated bacterial endophytes from resistant Yengambi KM5 (AAA) and susceptible banana cultivar Ney Poovan (AB) to determine the diversity of cultivable bacterial endophytes. Our study revealed the presence of 56 cultivable bacterial endophytes and 6 nectar-associated bacteria in YKM5 and 31 cultivable bacterial endophytes in Ney Poovan. The identified cultivable bacterial genera in YKM5 included

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“…Considering the same attempts made to bioprime, the seeds with B. amyloliquefaciens VB7 coupled with polymer coating reduced the chilli damping-off caused by Pythium ultimum [9]. A liquid formulation of B. amyloliquefaciens VB7 suppressed the incidence of carnation stem rot, Botrytis leaf blight of lillium under protected condition [3,10]. Soil drenching and foliar application with a liquid formulation of B. amyloliquefaciens VB7 reduced the necrosis disease of cotton caused by Tobacco streak virus (TSV) and disease severity of GBNV infecting tomato under field conditions through the induction of defense-related genes [1,2,11].…”

Section: Introductionmentioning

confidence: 99%

Mining the Genome of Bacillus velezensis VB7 (CP047587) for MAMP Genes and Non-Ribosomal Peptide Synthetase Gene Clusters Conferring Antiviral and Antifungal Activity

et al. 2021

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Chemical pesticides have an immense role in curbing the infection of plant viruses and soil-borne pathogens of high valued crops. However, the usage of chemical pesticides also contributes to the development of resistance among pathogens. Hence, attempts were made in this study to identify a suitable bacterial antagonist for managing viral and fungal pathogens infecting crop plants. Based on our earlier investigations, we identified Bacillus amyloliquefaciens VB7 as a potential antagonist for managing Sclerotinia sclerotiorum infecting carnation, tobacco streak virus infecting cotton and groundnut bud necrosis infecting tomato. Considering the multifaceted action of B. amyloliquefaciens VB7, attempts were made for whole-genome sequencing to assess the antiviral activity against tomato spotted wilt virus infecting chrysanthemum and antifungal action against Fusarium oxysporum f. sp. cubense (Foc). Genome annotation of the isolate B. amyloliquefaciens VB7 was confirmed as B. velezensis VB7 with accession number CP047587. Genome analysis revealed the presence of 9,231,928 reads with an average read length of 149 bp. Assembled genome had 1 contig, with a total length of 3,021,183 bp and an average G+C content of 46.79%. The protein-coding sequences (CDS) in the genome was 3090, transfer RNA (tRNA) genes were 85 with 29 ribosomal RNA (rRNA) genes and 21 repeat regions. The genome of B. velezensis VB7 had 506 hypothetical proteins and 2584 proteins with functional assignments. VB7 genome had the presence of flagellin protein FlaA with 987 nucleotides and translation elongation factor TU (Ef-Tu) with 1191 nucleotides. The identified ORFs were 3911 with 47.22% GC content. Non ribosomal pepide synthetase cluster (NRPS) gene clusters in the genome of VB7, coded for the anti-microbial peptides surfactin, butirosin A/butirosin B, fengycin, difficidin, bacillibactin, bacilysin, and mersacidin the Ripp lanthipeptide. Antiviral action of VB7 was confirmed by suppression of local lesion formation of TSWV in the local lesion host cowpea (Co-7). Moreover, combined application of B. velezensis VB7 with phyto-antiviral principles M. Jalapa and H. cupanioides increased shoot length, shoot diameter, number of flower buds per plant, flower diameter, and fresh weight of chrysanthemum. Further, screening for antifungal action of VB7 expressed antifungal action against Foc in vitro by producing VOC/NVOC compounds, including hexadecanoic acid, linoelaidic acid, octadecanoic acid, clindamycin, formic acid, succinamide, furanone, 4H-pyran, nonanol and oleic acid, contributing to the total suppression of Foc apart from the presence of NRPS gene clusters. Thus, our study confirmed the scope for exploring B. velezensis VB7 on a commercial scale to manage tomato spotted wilt virus, groundnut bud necrosis virus, tobacco streak virus, S. sclerotiorum, and Foc causing panama wilt of banana.

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Bacillus amyloliquefaciens alters the diversity of volatile and non-volatile metabolites and induces the expression of defence genes for the management of Botrytis leaf blight of Lilium under protected conditions

Cited by 14 publications

References 42 publications

Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Mechanisms of Action of Microbial Biocontrol Agents against Botrytis cinerea

Differential bacterial endophytome in Foc‐resistant banana cultivar displays enhanced antagonistic activity against Fusarium oxysporum f.sp. cubense (Foc)

Mining the Genome of Bacillus velezensis VB7 (CP047587) for MAMP Genes and Non-Ribosomal Peptide Synthetase Gene Clusters Conferring Antiviral and Antifungal Activity

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