Bacillomycin: An Antibiotic from Bacillus subtilis Active against Pathogenic Fungi

Landy, Maurice; Warren, George H.; RosenmanM, S. B; Colio, Lloyd G.

doi:10.3181/00379727-67-16367

Cited by 251 publications

(143 citation statements)

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“…The best results were obtained (a) in the Landy medium [36] supplemented with yeast extract in addition with D, L-alanine, and/or phenylalanine, and (b) in the glucose-starch-CaCO 3 (GSC) medium [34]. Figure 2a shows the MALDI-TOF mass spectrum of a culture supernatant of Paenibacillus polymyxa M-1 cultivated in liquid GSC medium in the range of m/z 860-1250 exhibiting the mass peaks of the fusaricidins (m/z 860-1050) and the polymyxins (m/z 1170-1230), which appeared in similar intensities.…”

Section: Demonstration Of the Fusaricidin Complex By Maldi-tof-msmentioning

confidence: 65%

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

Vater

Niu

Dietel³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Paenibacillus polymyxa-M1 is a potent producer of bioactive compounds, such as lipopeptides, polyketides, and lantibiotics of biotechnological and medical interest. Genome sequencing revealed nine gene clusters for nonribosomal biosynthesis of such agents. Here we report on the investigation of the fusaricidins, a complex of cyclic lipopeptides containing 15-guanidino-3-hydroxypentadecanoic acid (GHPD) as fatty acid component by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). More than 20 variants of these compounds were detected and characterized in detail. Mass spectrometric sequence analysis was performed by MALDI-LIFT-TOF/TOF fragment analysis. The obtained product ion spectra show a specific processing in the fatty acid part. GHPD is cleaved between the α-and ß-position yielding two fragments a and b, one bearing the end-standing guanidine group and another one comprising the residual two C-atoms of GHPD with the attached peptide moiety. The complete sequence of all fusaricidins was derived from sets of b n -and y n -ions. The fusaricidin complex can be divided into four lipopeptide families, three of them showing variations of the amino acid in position 3, Val or Ile for the first and Tyr or Phe for families 2 and 3, respectively. A collection of novel fusaricidins was detected differing from those of families 1-3 by an additional residue of 71 Da (family 4). LIFT-TOF/TOF fragment spectra of these species imply that in their peptide moiety, an Ala-residue is attached by an ester bond to the free hydroxyl group of Thr 4 . More than 10 novel fusaricidins were characterized mass spectrometrically.

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Section: Demonstration Of the Fusaricidin Complex By Maldi-tof-msmentioning

confidence: 65%

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

Vater

Niu

Dietel³

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…marinus B-9987 wild-type and overexpression strains were fermented in Landy medium [29] using a two-stage fermentation process: a single colony of the B. marinus B-9987 strains was first inoculated into 50 mL of seed medium in a 250-mL flask and incubated at 37°C, 200 r min 1 for 12 h. The resulting seed culture (5 mL) was used to inoculate 50 mL of production medium in a 250-mL flask and incubated at 37°C, 200 r min 1 for additional 12 h. The fermentation broth was centrifuged at 5000×g for 10 min to remove the cells. The resulting supernatant was extracted twice with equal volume of EtOAc.…”

Section: Fermentation and Hplc Analysismentioning

confidence: 99%

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

Liu

Zheng

Zhan

et al. 2014

Sci. China Life Sci.

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Marine-derived Bacillus strains have been proved to be a very promising source for natural product leads. However, transformation of environmental strains is much more difficult than that of domesticated strains. Here, we report the development of an efficient and robust electroporation-based transformation system for marine-derived Bacillus marinus B-9987, which is a macrolactin antibiotics producer and a very promising biological control agent against fungal plant diseases. The transformation efficiency was greatly enhanced 10 3 -fold by using unmethylated plasmid to bypass modification-restriction barrier, and using glycine betaine to protect cells from electrical damages during electroporation. Addition of HEPES and 2 mmol L 1 MgCl 2 further improved the efficiency by additional 2-fold, with a maximum value of 7.1×10 4 cfu/µg pHT3101. To demonstrate the feasibility and efficiency of the protocol, a green fluorescent protein reporter system was constructed; furthermore, phosphopantetheinyl transferase gene sfp, which is essential to the biosynthesis of polyketides and nonribosomal peptides, was overexpressed in B-9987, leading to increased production of macrolactin A by about 1.6-fold. In addition, this protocol is also applicable to marine-derived Bacillus licheniforms EI-34-6, indicating it could be a reference for other undomesticated Bacillus strains. To our knowledge, this is the first report regarding the transformation of marine-derived Bacillus strain. marine-derived, Bacillus marinus, electroporation, macrolactin, green fluorescent protein, phosphopantetheinyl transferase Citation:Liu Y, Zheng H, Zhan GH, Qin W, Tian L, Li WL. Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain.

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“…Furthermore, the lytic effect of cell-free filtrates was destroyed by heat while the fungistatic effect of incorporating these filtrates into agar was, with one exception, also destroyed by heat. Enzymatic activity is destroyed by heat (Horikoshi and Iida 1958), although a range of heat-stable antibiotics have been reported (Foster and Woodruff 1946;Landy et al 1948). It is possible that, with 1. pastinacae, both types of mycolysis are implicated but the present research does not elucidate this problem.…”

Section: Discussionmentioning

confidence: 99%

The Survival in Soil of Itersonilia Pastinacae Channon, the Cause of Parsnip Canker

Smith¹

1967

Aust. Jnl. Of Bio. Sci.

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Bacillomycin: An Antibiotic from Bacillus subtilis Active against Pathogenic Fungi

Cited by 251 publications

References 0 publications

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry

Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain

The Survival in Soil of Itersonilia Pastinacae Channon, the Cause of Parsnip Canker

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