Bacilli in the Biocontrol of Mycotoxins

Subbaiah, Chalivendra C.; Ham, Jong Hyun

doi:10.1007/978-3-030-15175-1_3

Cited by 5 publications

(3 citation statements)

References 64 publications

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“…Biocontrol of mycotoxins in the agricultural produce by the application of Bacillus spp. has been reviewed (118). Moreover, these approaches have received increased attention as they are safe and effective than the use of chemicals for mycotoxin contamination control (110).…”

Section: Reduction Of Mycotoxin In Agricultural Producementioning

confidence: 99%

Bacilli as sources of agrobiotechnology: recent advances and future directions

Dame

Rahman

Islam

2021

Green Chemistry Letters and Reviews

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The group bacilli represent the Gram-positive ubiquitous spore-forming bacteria. Their diversity, versatility and the ability of producing diverse secondary metabolites including enzymes created enormous potential for applications in agriculture, biotechnology, environment and medicine. The bacilli are considered as one of the most studied groups of bacteria providing plant growth-promotion and biocontrol of multiple diseases reflecting their vital role in enhancing plants' tolerance to biotic and abiotic stresses. Some of the Bacillus species are available commercially as phytostimulants, biopesticides, and biofertilizers. Genetically engineered plants such as maize, cotton, brinjal with endotoxins producing genes from Bacillus thuringiensis (Bt) has revolutionized agriculture. Many of these applications have been widely adopted in various fields as viable and environmentally friendly alternatives of synthetic chemical fertilizers and pesticides. A better understanding of the biology, ecology, and mechanisms of action of the beneficial strains of bacilli are needed for the development of products to support green biotechnology in agriculture and industries. This report comprehensively reviewed the applications of bacilli in agriculture and industry and discussed their potentials for the development of new products of biotechnological implications.

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Section: Reduction Of Mycotoxin In Agricultural Producementioning

confidence: 99%

Bacilli as sources of agrobiotechnology: recent advances and future directions

Dame

Rahman

Islam

2021

Green Chemistry Letters and Reviews

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“…In addition to amino acid substitutions, the nature, length and configuration of the fatty-acid chain contribute to the variability. Their structural diversity and cyclic configuration confer them with broad-spectrum and environmentally stable antibiosis activity against bacteria, fungi, insects, protozoa and even human tumor cell lines [ 45 , 46 ]. CLPs are produced mostly by Pseudomonas , Bacillus and Streptomyces spp.…”

Section: Cyclic Lipopeptide Surfactantsmentioning

confidence: 99%

Microbial Toxins in Insect and Nematode Pest Biocontrol

Subbaiah

2021

IJMS

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Invertebrate pests, such as insects and nematodes, not only cause or transmit human and livestock diseases but also impose serious crop losses by direct injury as well as vectoring pathogenic microbes. The damage is global but greater in developing countries, where human health and food security are more at risk. Although synthetic pesticides have been in use, biological control measures offer advantages via their biodegradability, environmental safety and precise targeting. This is amply demonstrated by the successful and widespread use of Bacillusthuringiensis to control mosquitos and many plant pests, the latter by the transgenic expression of insecticidal proteins from B. thuringiensis in crop plants. Here, I discuss the prospects of using bacterial and fungal toxins for pest control, including the molecular basis of their biocidal activity.

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“…Among these, Bacillus spp. is considered one of best biocontrol agents for mycotoxin production by inhibition of fungal growth and mycotoxin detoxification. , Bacillus velezensis is a recently identified bacterial strain capable of effectively degrading OTA, ZEN, and aflatoxin and shows promise as a non-toxic probiotic for the food industry. − However, the mechanism of OTA degradation has not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Bacillus velezensis IS-6 Nudix Hydrolase Nh-9 Involved in Ochratoxin A Detoxification by Transcriptomic Profiling and Functional Verification

Jahan

Tai

et al. 2023

J. Agric. Food Chem.

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Contamination of foods and feeds with Ochratoxin A (OTA) is a global problem, and its detoxification is challenging. In this study, Bacillus velezensis IS-6 culture isolate supernatant degraded 1.5 g/mL OTA by 89% after 24 h of incubation at 37 °C, whereas viable cells and intra-cell extracts were less effective. The OTA degradation by B. velezensis IS-6 was an enzymatic process mediated by the culture supernatant. The degradation activity was optimal at 37 °C and pH 7.0, and Fe 2+ and Cu 2+ ions enhanced the OTA degradation. The LC−MS/MS analysis confirmed that structure of OTA was modified, resulting in the production of OTα that was less toxic than OTA. The transcriptomic analysis of B. velezensis IS-6 showed that 38 differentially expressed genes (DEGs) were significantly up-regulated, and 24 DEGs were down-regulated after treatment with OTA. A novel OTA degradation enzyme Nudix hydrolase Nh-9 was successfully cloned and characterized from the up-regulated genes. The recombinant Nh-9 enzyme was overexpressed in Escherichia coli BL21 and purified by affinity chromatography, exhibiting 68% degradation activity against 1.0 μg/ mL OTA at 37 °C in 24 h. The degraded product by the Nh-9 enzyme was identified as the less toxic OTα by LC−MS/MS. According to the findings, it can be inferred that Nh-9 is the main OTA-degrading enzyme in B. velezensis IS-6. Furthermore, OTA may be co-degraded by Nh-9, carboxylesterase, signal peptidase, and other degrading agents that are yet to be discovered in this strain.

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Bacilli in the Biocontrol of Mycotoxins

Cited by 5 publications

References 64 publications

Bacilli as sources of agrobiotechnology: recent advances and future directions

Bacilli as sources of agrobiotechnology: recent advances and future directions

Microbial Toxins in Insect and Nematode Pest Biocontrol

Identification of a Novel Bacillus velezensis IS-6 Nudix Hydrolase Nh-9 Involved in Ochratoxin A Detoxification by Transcriptomic Profiling and Functional Verification

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