Bacillary Necrosis, a Disease of Larval and Juvenile Bivalve Mollusks I. Etiology and Epizootiology

Tubiash, Haskell S.; Chanley, Paul; Leifson, Einar

doi:10.1128/jb.90.4.1036-1044.1965

Cited by 165 publications

(80 citation statements)

References 7 publications

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“…After removing the unchallenged oysters from the experimental trays, we subjected the remaining eight oysters/family/tray to a bacterial challenge by turning off the incoming water flow and adding log‐phase V. tubiashii culture (ATCC 19106) (Tubiash et al. 1965; 1970) to the trays for 3 h in static water, after which we restored water flow and allowed the bacteria to flush gradually from the trays.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome response of the Pacific oyster (Crassostrea gigas) to infection with Vibrio tubiashii using cDNA AFLP differential display

et al. 2009

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We used qualitative complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) differential display analysis and real-time, quantitative PCR (RT-qPCR) to identify genes in the Pacific oyster Crassostrea gigas, whose transcription either changes in response to exposure to a pathogenic bacterium (Vibrio tubiashii) or varies between families known to differ in sensitivity to heat stress, before and at 12 and 36 h after bacterial exposure at a temperature of 25 degrees C. These conditions simulate those associated with summer mortality syndrome, a poorly understood cause of massive mortalities in cultured Pacific oysters in North America, Asia and Europe. Using 32 AFLP primer pairs, we identified 92 transcript-derived fragments that are qualitatively differentially expressed. We then cloned and sequenced 14 of these fragments, designed fragment-specific primers and quantified their transcription patterns using RT-qPCR. Most of the differences in transcription patterns between stress-tolerant and stress-sensitive families were evident before bacterial exposure, and genes that responded to bacterial exposure did so in parallel between stress-sensitive and stress-tolerant families. Blast searches of sequence databases revealed that these fragments represent genes involved in immune response as well as genes related to metabolic processes. Our data support the hypothesis that family level differences in resistance to stress in Pacific oysters are largely attributable to constitutive differences in gene transcription or 'general vigour' that are detectable before and maintained after infection, rather than being due to induced responses at the transcriptome level.

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Section: Methodsmentioning

confidence: 99%

Transcriptome response of the Pacific oyster (Crassostrea gigas) to infection with Vibrio tubiashii using cDNA AFLP differential display

et al. 2009

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“…Vibriosis is the main disease currently hindering the success of the hatchery culture of bivalves. Different species of Vibrio have been described as opportunistic pathogens associated with mortalities during larval development: V. alginolyticus , V. anguillarum , V. neptunius , V. pectenicida , V. splendidus and V. tubiashii (Tubiash, Chanley & Leifson ; Brown ; Jeffries ; Riquelme et al . , ; Nicolas et al .…”

Section: Introductionmentioning

confidence: 99%

Vibrios in hatchery cultures of the razor clam, Solen marginatus (Pulteney)

Prado

Dubert

Costa

et al. 2013

Journal of Fish Diseases

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Hatchery culture of the razor clam, Solen marginatus (Pulteney), has recently been developed in Galicia (NW Spain). However, recurrent episodes of mortalities of larval and post-larval cultures have been recorded during the course of various studies. The disease signs were similar to those described for other bivalve species in outbreaks caused by bacteria of the genus Vibrio. In this article, we present the results of microbiological monitoring of two batches of razor clams with different survival rates. All fermentative isolates were identified as members of the Splendidus clade within the genus Vibrio. Some of these isolates, identified as Vibrio splendidus-like, were clearly associated with the batch suffering mortalities, indicating their possible role as pathogens. Similar strains were found in the broodstock, suggesting vertical transmission of these bacteria. This is the first study of the microbiota associated with hatchery culture of S. marginatus, and the results will provide useful information for the optimization of a protocol for hatchery culture of this bivalve species.

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“…No specific clinical symptoms such as adhesion of ciliates and velum necrosis on the dead larvae were observed microscopically (Figure a). Specific clinical signs of V. coralliilyticus, V. tubiashii and OVVD infection such as velum necrosis, velar degeneration with loss of cilia and velar epithelium (Elston & Wilkinson, ; Sugumar et al, ; Tubiash et al, ) (Figure b, unpublished data) were not observed. In addition, protozoans including parasitic organisms were also not observed except feeding phytoplankton.…”

Section: Resultsmentioning

confidence: 94%

“…During mass mortality of the bay scallop larvae in the southern regions of Korea, it was observed that 5–10‐day‐old larva sank to the bottom of the tank. Based on this observation, this study focused on OsHV‐1, OVVD, V. coralliilyticus and V. tubiashii infections which were have been reported to demonstrate similar symptoms (Elston & Wilkinson, ; Farley et al, ; Rodgers, Arzul, Carrasco, & Furones Nozal, ; Sugumar, Nakai, Hirata, Matsubara, & Muroga, ; Tubiash, Chanley, & Leifson, ).…”

Section: Resultsmentioning

confidence: 98%

“…Although, it could not be confirmed that the isolated Vibrio sp. was not the causative agent of the mass mortality, V. coralliilyticus and V. tubiashii which are reported to cause massive mortality of bivalve larvae were not detected in PCR and the specific clinical signs such as velar degeneration with loss of cilia and velar epithelial cells were not observed (Sugumar et al, ; Tubiash et al, ). Therefore, we ruled out the possibility of the Vibrio sp.…”

Section: Resultsmentioning

confidence: 99%

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Mass mortality in Korean bay scallop (Argopecten irradians) associated with Ostreid Herpesvirus‐1 μVar

Kim

Jun²,

Giri

et al. 2019

Transbound Emerg Dis

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Summary Since November 2017, mass mortalities of larvae of bay scallop (Argopecten irradians) were reported in hatcheries located at the southern area of Republic of Korea. Over 90% of larvae aged 5–10 days sank to the bottom of the tank and died. The hatcheries could not produce spat, and thus artificial seed production industry incurred huge losses. We identified Ostreid Herpesvirus‐1 μVar (OsHV‐1 μVar) associated with mass mortality by PCR, sequencing and transmission electron microscopy (TEM). All the samples were positive for OsHV‐1 μVar with 99% sequence identity to previously reported OsHV‐1 μVar sequences. Partial sequence of ORF‐4 of OsHV‐1 detected in this study was more closely related to sequences isolated from Europe. This is the first report to confirm the mortality caused by an OsHV‐1 infection in the bay scallop.

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Bacillary Necrosis, a Disease of Larval and Juvenile Bivalve Mollusks I. Etiology and Epizootiology

Cited by 165 publications

References 7 publications

Transcriptome response of the Pacific oyster (Crassostrea gigas) to infection with Vibrio tubiashii using cDNA AFLP differential display

Transcriptome response of the Pacific oyster (Crassostrea gigas) to infection with Vibrio tubiashii using cDNA AFLP differential display

Vibrios in hatchery cultures of the razor clam, Solen marginatus (Pulteney)

Mass mortality in Korean bay scallop (Argopecten irradians) associated with Ostreid Herpesvirus‐1 μVar

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