Baboon (Papio ursinus) single fibre contractile properties are similar to that of trained humans

Dada, Suhail; Henning, Franclo; Feldmann, Daneil; Kohn, Tertius Abraham

doi:10.1007/s10974-019-09509-x

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…The fiber type and cross‐sectional area (CSA) were determined using immunohistochemistry as described by Dada et al (2018). Briefly, frozen sections were blocked with 5% bovine serum albumin (BSA) dissolved in 0.1 M phosphate buffered saline (PBS) pH 7.4 and incubated overnight at 4°C with primary antibodies, diluted 1:50 in PBS (Developmental Studies Hybridoma Bank, Iowa City, IA) against MHC I (BA‐D5; IgG2b), MHC IIA (SC‐71; IgG1) and MHC IIX (6H1, IgM).…”

Section: Methodsmentioning

confidence: 99%

A novel description of the Vastus lateralis morphology of the Temminck's ground pangolin (Manis temminckii)

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Breed

Hughes

et al. 2022

The Anatomical Record

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The pangolin (Manidae family) is the world's most trafficked animal, yet very little is known about its physiology and metabolism primarily due to its inconspicuous and solitary nature. Skeletal muscle samples from the Vastus lateralis were collected postmortem from a single female Temminck's ground pangolin (Manis temminckii). Samples were analyzed for fiber type composition, fiber size and myosin heavy chain isoform content. The oxidative and glycolytic metabolic capacity was determined through citrate synthase, 3-hydroxyacetyl co A dehydrogenase, creatine kinase, lactate dehydrogenase, phosphofructokinase and glycogen phosphorylase enzyme activities. Lastly, antioxidant capacity was determined through superoxide dismutase and catalase enzyme activities, and the total antioxidant capacity. The pangolin metabolic profile

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Section: Methodsmentioning

confidence: 99%

A novel description of the Vastus lateralis morphology of the Temminck's ground pangolin (Manis temminckii)

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Breed

Hughes

et al. 2022

The Anatomical Record

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“…In addition, it also led to the discovery that human fibers, previously classified as type IIB using the conventional ATPase stability method, in fact contained mRNA transcripts 94% homologous to that of the rat MHC IIX (Schiaffino & Reggiani, 1996). This was further confirmed with immunohistochemistry, single muscle fiber electrophoresis and Western blotting when the anti-MHC IIX antibody (6H1) strongly reacted with fibers from other species (including that from humans), but the anti-MHC IIB antibody (BF-F3) showed no reactivity (Dada et al, 2018;Kohn, 2014;Kohn, Burroughs, et al, 2011;Maccatrozzo, Patruno, Toniolo, Reggiani, & Mascarello, 2004). Thus, most mammals express the MHC IIX isoform in their large muscles and not the MHC IIB.…”

Section: Muscle Fiber Typementioning

confidence: 63%

“…lions, cheetahs, tigers), antelope species (e.g. black wildebeest, springbok) and baboons (Dada, Henning, Feldmann, & Kohn, 2018;Hyatt, Roy, Rugg, & Talmadge, 2010;Kohn, 2014;Kohn, Burroughs, Hartman, & Noakes, 2011). These included monoclonal antibodies against mammalian isoforms namely N2.261 (reacting with MHC I and MHC IIA), A4.74 (reacting with MHC IIA and IIX), BF-35 (reacting with MHC I and IIA), 6H1 (reacting with MHC IIX), BA-D5 (reacting with MHC I), SC-71 (reacting with MHC IIA and IIX) and BF-F3 (reacting with MHC IIB).…”

Section: Histologymentioning

confidence: 99%

Muscle fiber type and metabolic profiles of four muscles from the African black ostrich

et al. 2023

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“…Although Schiaffino and Reggiani (2011) suggested that larger animals (including humans) predominantly have higher number of Type I and IIA fibers, this theory does not hold true anymore. While the genotype plays an important role in determining stature of an animal, the work conducted by Kohn et al on various wild animal species with different body sizes (lion, caracal, springbok, black wildebeest, baboon) showed that fiber type composition was not predicted by the weight of the animal (Curry et al, 2012; Dada et al, 2018; Kohn, 2014; Kohn et al, 2011; Kohn, Hoffman, et al, 2007). This finding is also supported by the current cohort of dog breeds where no significant correlation was found between their weight and fiber type.…”

Section: Discussionmentioning

confidence: 99%

Fiber type and metabolic characteristics of skeletal muscle in 16 breeds of domestic dogs

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Schoeman

Steyl

et al. 2023

The Anatomical Record

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The domestic dog (Canis lupus familiaris) species comprises hundreds of breeds, each differing in physical characteristics, behavior, strength, and running capability. Very little is known about the skeletal muscle composition and metabolism between the different breeds, which may explain disease susceptibility. Muscle samples from the triceps brachii (TB) and vastus lateralis (VL) were collected post mortem from 35 adult dogs, encompassing 16 breeds of varying ages and sex. Samples were analyzed for fiber type composition, fiber size, oxidative, and glycolytic metabolic capacity (citrate synthase [CS], 3‐hydroxyacetyl‐coA dehydrogenase [3HAD], creatine kinase [CK], and lactate dehydrogenase [LDH] enzyme activities). There was no significant difference between the TB and VL in any of the measurements. However, there were large intra species variation, with some variables confirming the physical attributes of a specific breed. Collectively, type IIA was the predominant fiber type followed by type I and type IIX. The cross‐sectional areas (CSA) of the fibers were all smaller when compared to humans and similar to other wild animals. There was no difference in the CSA between the fiber types and muscle groups. Metabolically, the muscle of the dog displayed high oxidative capacity with high activities for CS and 3HAD. Lower CK and higher LDH activities than humans indicate a lower and higher flux through the high energy phosphate and glycolytic pathways, respectively. The high variability found across the different breeds may be attributed to genetics, function or lifestyle which have largely been driven through human intervention. This data may provide a foundation for future research into the role of these parameters in disease susceptibility, such as insulin resistance and diabetes, across breeds.

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Baboon (Papio ursinus) single fibre contractile properties are similar to that of trained humans

Cited by 5 publications

References 34 publications

A novel description of the Vastus lateralis morphology of the Temminck's ground pangolin (Manis temminckii)

A novel description of the Vastus lateralis morphology of the Temminck's ground pangolin (Manis temminckii)

Muscle fiber type and metabolic profiles of four muscles from the African black ostrich

Fiber type and metabolic characteristics of skeletal muscle in 16 breeds of domestic dogs

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