Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis

Wang, Hui; Wang, Yao; Huang, Ji-Lei; Xu, Bin; Chen, Jun-Hu; Dai, Jianfeng; Zhou, Xiao‐Nong

doi:10.3389/fimmu.2020.01437

Cited by 9 publications

(7 citation statements)

References 47 publications

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“…Hb concentration, body weight and body temperature are indicators that reflect the severity of the disease, and they may be distinctly different when obvious protections are induced in the immune group [ 23 ]. In this study, we found that Hb concentration was significantly higher in the immune group than in the control group at days 3 and 6 after passive immunity regarding Bm infection.…”

Section: Discussionmentioning

confidence: 99%

A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection

Wang,

Zhang,

Zhang

et al. 2023

Parasites Vectors

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Background The protozoan parasite Babesia microti that causes the zoonotic disease babesiosis resides in the erythrocytes of its mammalian host during its life-cycle. No effective vaccines are currently available to prevent Babesia microti infections. Methods We previously identified a highly seroactive antigen, named Bm8, as a B. microti conserved erythrocyte membrane-associated antigen, by high-throughput protein chip screening. Bioinformatic and phylogenetic analysis showed that this membrane-associated protein is conserved among apicomplexan hemoprotozoa, such as members of genera Babesia, Plasmodium and Theileria. We obtained the recombinant protein Bm8 (rBm8) by prokaryotic expression and purification. Results Immunofluorescence assays confirmed that Bm8 and its Plasmodium homolog were principally localized in the cytoplasm of the parasite. rBm8 protein was specifically recognized by the sera of mice infected with B. microti or P. berghei. Also, mice immunized with Bm8 polypeptide had a decreased parasite burden after B. microti or P. berghei infection. Conclusions Passive immunization with Bm8 antisera could protect mice against B. microti or P. berghei infection to a certain extent. These results lead us to hypothesize that the B. microti conserved erythrocyte membrane-associated protein Bm8 could serve as a novel broad-spectrum parasite vaccine candidate since it elicits a protective immune response against Babesiosis and Plasmodium infection. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection

Wang,

Zhang,

Zhang

et al. 2023

Parasites Vectors

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“…In our previous proteome high-throughput screening, some B. microti proteins with high antigenicity were identi ed [18,19]. The protection and diagnostic potential of one secreted protein BmSP44 was evaluated [20]. Bioinformatics analysis showed that an erythrocyte membrane associated protein8 of B. microti (named as Bm8 in this study) was conserved among the apicomplexa parasites such as Plasmodium spp.…”

Section: Introductionmentioning

confidence: 90%

A Babesia microti conserved membrane-associated protein Bm8 elicits partial protection against Babesia and Plasmodium infection

Wang

Zhang

et al. 2023

Preprint

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The protozoan parasite Babesia microti that causes the zoonoses, babesiosis interacts with the host erythrocytes during its life cycle. So far, no effective vaccines are available to prevent Babesia infections. In this study, we identified a B. microti conserved erythrocyte membrane-associated antigen, Bm8, as a high seroreactivity antigen. Bioinformatic and phylogenetic analysis showed that this membrane-associated protein is conserved among apicomplexan hemoprotozoa, such as Babesia, Plasmodium, and Theileria. The recombinant protein Bm8 (rBm8) was obtained by prokaryotic expression and purification. Immunofluorescence assays (IFA) confirmed that Bm8 and its plasmodium homolog is localized principally in the cytoplasm of the parasites. rBm8 protein can be specifically recognized by the sera of mice infected with B. microti or P. berghei. Further, mice immunized with Bm8 polypeptide had a decreased parasite burdenafter B. microti or P. berghei infection. Accordingly, passive immunization withBm8 antisera also partially protected mice against B. microti or P. berghei infection. Thus, wepropose that the B. microticonserved erythrocyte membrane-associated protein Bm8 might serve as a novel broad-spectrum parasite vaccine candidate having a protective immune response against Babesiosis and Plasmodium infection.

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“…Mice immunized with BmSP44 demonstrated a significant difference in parasitemia when compared to the control group. Nevertheless, both vaccinated and control group mice still experienced high peak parasitemia [ 47 ].…”

Section: Advances In B Microti Vaccine Developmentmentioning

confidence: 99%

Advances in Babesia Vaccine Development: An Overview

et al. 2023

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Babesiosis is a tick-borne zoonotic disease, which is caused by various species of intracellular Babesia parasite. It is a problem not only for the livestock industry but also for global health. Significant global economic losses, in particular in cattle production, have been observed. Since the current preventive measures against babesiosis are insufficient, there is increasing pressure to develop a vaccine. In this review, we survey the achievements and recent advances in the creation of antibabesiosis vaccine. The scope of this review includes the development of a vaccine against B. microti, B. bovis, B. bigemina, B. orientalis and B. divergens. Here, we present different strategies in their progress and evaluation. Scientists worldwide are still trying to find new targets for a vaccine that would not only reduce symptoms among animals but also prevent the further spread of the disease. Molecular candidates for the production of a vaccine against various Babesia spp. are presented. Our study also describes the current prospects of vaccine evolution for successful Babesia parasites elimination.

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Babesia microti Protein BmSP44 Is a Novel Protective Antigen in a Mouse Model of Babesiosis

Cited by 9 publications

References 47 publications

A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection

A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection

A Babesia microti conserved membrane-associated protein Bm8 elicits partial protection against Babesia and Plasmodium infection

Advances in Babesia Vaccine Development: An Overview

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