Babesia divergens apical membrane antigen-1 (BdAMA-1): A poorly polymorphic protein that induces a weak and late immune response

Moreau, Emmanuelle; Bonsergent, Claire; Dybiat, Iman Al; González, Luís Miguel; Lobo, Cheryl A.; Montero, Estrella; Malandrin, Laurence

doi:10.1016/j.exppara.2015.04.024

Cited by 17 publications

(13 citation statements)

References 33 publications

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“…Antibody production against the extracellular domain of BdAMA-1 is weak and late and it includes both IgG1 and IgG2. These results indicate that even if BdAMA-1 may not be an immunodominant antigen, it could induce a mixed type 1 and type 2 immune response [74]. AMA-1 orthologs have been also reported in other Babesia species [75][76][77][78].…”

Section: Babesia Sppmentioning

confidence: 57%

“…-IFN-γ expression in CD4 + T cells [66] Babesia spp. Rhoptry-Associated Protein (RAP-1) Antigen-specific IgG and IFN-γ release [72] Heat-shock protein 20 (Hsp 20) T cell proliferation and IFN-γ production [71] B. microti surface antigen (BMSA) Th17 and Th2 activation and IgG1 secretion [73] B. divergens Apical Membrane Antigen-1 (BdAMA-1) IgG1, IgG2, Th1 and Th2 activation [74] B. bovis rhoptry neck protein 2 (RON2) Induction of partially neutralizing antibodies that blocked the invasion process [79] Chimeric multi-antigen Antigen-specific IgG1, CD4 + and CD8 + IFN-γ + [81][82][83] Theileria parva Tp1-Tp2-Tp4-Tp5-Tp7-Tp8 CD8 + T-cell responses [92,[94][95][96][97][98][99][100][101] p67 Ab production [102] Polymorphyc immunodominant molecule (PIM) Production of Ab with neutralizing activity [103,104] p150 Ab production…”

Section: Rp778mentioning

confidence: 99%

See 1 more Smart Citation

Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines

Torina

Blanda

Villari

et al. 2020

IJMS

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Tick-transmitted pathogens cause infectious diseases in both humans and animals. Different types of adaptive immune mechanisms could be induced in hosts by these microorganisms, triggered either directly by pathogen antigens or indirectly through soluble factors, such as cytokines and/or chemokines, secreted by host cells as response. Adaptive immunity effectors, such as antibody secretion and cytotoxic and/or T helper cell responses, are mainly involved in the late and long-lasting protective immune response. Proteins and/or epitopes derived from pathogens and tick vectors have been isolated and characterized for the immune response induced in different hosts. This review was focused on the interactions between tick-borne pathogenic hemoparasites and different host effector mechanisms of T- and/or B cell-mediated adaptive immunity, describing the efforts to define immunodominant proteins or epitopes for vaccine development and/or immunotherapeutic purposes. A better understanding of these mechanisms of host immunity could lead to the assessment of possible new immunotherapies for these pathogens as well as to the prediction of possible new candidate vaccine antigens.

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Section: Babesia Sppmentioning

confidence: 57%

Section: Rp778mentioning

confidence: 99%

Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines

Torina

Blanda

Villari

et al. 2020

IJMS

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“…BQ1 (Lintan) infection. However, kinetic studies of humoral responses against B. divergens AMA-1 indicated that antibody production against extracellular DI and DII of BdAMA-1 is weak and late, between more than 3 and 5 months post-infection during experimental infection of sheep by B. divergens (Moreau et al 2015). Rabbit sera against the BLTrAMA-1-DI/DII protein did not react with uninfected sheep RBCs and the serum obtained prior to infection of sheep did not react with BLTrAMA-1 in Western-blot analyses.…”

Section: Discussionmentioning

confidence: 99%

“…BQ1 (Lintan) and/or other isolates of Babesia cf. motasi in China, as AMA-1 is considered highly conserved among different isolates of the same species (Torina et al 2010; Moreau et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, characterization and antigenicity ofBabesiasp. BQ1 (Lintan) (Babesiacf.motasi) apical membrane antigen-1 (AMA-1)

et al. 2016

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Apical membrane antigen-1 (AMA-1) has been described as a potential vaccine candidate in apicomplexan parasites. Here we characterize the ama-1 gene. The full-length ama-1 gene of Babesia sp. BQ1 (Lintan) (BLTAMA-1) is 1785 bp, which contains an open reading frame (ORF) encoding a 65-kDa protein of 594 amino acid residues; by definition, the 5' UTR precedes the first methionine of the ORF. Phylogenetic analysis based on AMA-1 amino acid sequences clearly separated Piroplasmida from other Apicomplexa parasites. The Babesia sp. BQ1 (Lintan) AMA-1 sequence is most closely associated with that of B. ovata and B. bigemina, with high bootstrap value. A recombinant protein encoding a conserved region and containing ectodomains I and II of BLTAMA-1 was constructed. BLTrAMA-1-DI/DII proteins were tested for reactivity with sera from sheep infected by Babesia sp. BQ1 (Lintan). In Western-blot analysis, native Babesia sp. BQ1 (Lintan) AMA-1 proteins were recognized by antibodies raised in rabbits against BLTrAMA-1 in vitro. The results of this study are discussed in terms of gene characterization, taxonomy and antigenicity.

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“…In Babesia species, AMA-1 as been described as a lowdiversity protein; in B. divergens, ama-1 was sequenced from nine isolates from France, in which only two punctual mutations were observed compared with the reference strain [71], in B. bovis, the analysis of Sri Lankan strains showed that ama-1 is a conserved member with about 95% of identity; the msa genes of this strains were mapped and showed variability. As mentioned previously, msa family is highly polymorphic.…”

Section: Ama-1mentioning

confidence: 99%

Genome-Based Vaccinology Applied to Bovine Babesiosis

Mosqueda¹,

Hernández-Silva²,

MarioHidalgo-Ruiz³

2018

Farm Animals Diseases, Recent Omic Trends and New Strategies of Treatment

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Genomics approaches in veterinary research have been a very useful tool to identify candidates with potential to be used in prevention of animal diseases. In Babesia, genome information analysis has elucidated a wide variety of protein families and some members are described in this chapter. Here, we present some of the most recent studies about B. bovis and B. bigemina genomes where some proteins have been identified with potential to prevent infections by these parasites.

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Babesia divergens apical membrane antigen-1 (BdAMA-1): A poorly polymorphic protein that induces a weak and late immune response

Cited by 17 publications

References 33 publications

Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines

Immune Response to Tick-Borne Hemoparasites: Host Adaptive Immune Response Mechanisms as Potential Targets for Therapies and Vaccines

Molecular cloning, characterization and antigenicity ofBabesiasp. BQ1 (Lintan) (Babesiacf.motasi) apical membrane antigen-1 (AMA-1)

Genome-Based Vaccinology Applied to Bovine Babesiosis

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