1989
DOI: 10.1016/0014-5793(89)80124-3
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Ba2+‐sensitive K+ channels in luminal‐membrane vesicles from pars convoluta of rabbit proximal tubule

Abstract: This paper describes properties of 8ORb + fluxes through a novel K + channel in luminal-membrane vesicles isolated from pars convoluta of rabbit proximal tubule. The uptake of 86Rb + into potassium salt loaded vesicles was specifically inhibited by Ba 2+. The isotope accumulation is driven by an electrical diffusion potential as shown in experiments using these membrane vesicles loaded with anions of different membrane permeability and was as follows: gluconate > SO~4 -> CI-. Furthermore, the vesicles containi… Show more

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Cited by 6 publications
(4 citation statements)
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“…However, in the proximal tubule there is now direct evidence for the existence of K+ channels both in luminal and basolateral membranes [l-4]. Recently, also nonselective cation channels which discriminate poorly between Na + and K + ions have been observed in luminal membranes [5,6] as well as basolateral membranes [7-91. In a previous paper we characterized K + channels in luminal membranes of rabbit proximal straight tubule (pars recta) [3]. In the present communication we describe the properties of a K+ -selective channel in basolateral membranes from pars convoluta.…”
Section: Introductionmentioning
confidence: 82%
See 1 more Smart Citation
“…However, in the proximal tubule there is now direct evidence for the existence of K+ channels both in luminal and basolateral membranes [l-4]. Recently, also nonselective cation channels which discriminate poorly between Na + and K + ions have been observed in luminal membranes [5,6] as well as basolateral membranes [7-91. In a previous paper we characterized K + channels in luminal membranes of rabbit proximal straight tubule (pars recta) [3]. In the present communication we describe the properties of a K+ -selective channel in basolateral membranes from pars convoluta.…”
Section: Introductionmentioning
confidence: 82%
“…Influx of ssRb+ was measured in K+ (55 mM)-loaded vesicles, analogous to flux-measurement procedures reported previously [3,15]. Dowex AGSOW-X8 columns were prepared in 1 ml tuberculin syringes.…”
Section: Assay Of Rb+ Transportmentioning
confidence: 99%
“…To obtain further information on K ϩ conductances in both the apical and basolateral membranes, 86 Rb ϩ efflux experiments were performed on gill-cell monolayers grown on permeable filters. In various epithelial cells (1,20), 86 Rb ϩ has been shown to be a good indicator for K ϩ movement. Moreover, the substitution of KCl by RbCl in gill cells did not change the whole cell currents induced by the hypotonic shock (data not given).…”
Section: Discussionmentioning
confidence: 99%
“…In previous papers we have demonstrated K ÷ and Na ÷ ion channels in membrane vesicles isolated from outer cortex (pars convoluta) and outer medulla (pars recta) of rabbit proximal tubule [6][7][8][9].…”
Section: Introductionmentioning
confidence: 94%