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Abstract

ABSTRACT:The present study was designed to evaluate the effect of Helix aspersa crude extract on colonic injury resulting from hyperhomocysteinemia induced by feeding rat's high methionine diet. Wistar albino rats (200-250g) were divided into four groups. Hyperhomocysteinemia (Hhcy) was induced by methionine treatment (1g/kg/day) for 15 days. M group received only Methionine, MH and MP received Methionine and Helix aspersa Crude Extract (8g/kg/day) or Prednisolone (4 mg/kg/day) respectively at the 8th day until the end of experimentation. Prednisolone was used as a standard drug. At the end plasma homocysteine and Creactive protein were evaluated, followed by colon histopathological investigations. Methionine treatment induced significant increase (P <0.001) in homocysteine and CRP levels in plasma as compared to control rats. Helix aspersa crude extract induces significant decrease of homocysteine (P <0.001) and CRP (P <0.01) levels as compared with experimental control group. The results were comparable to those obtained with prednisolone, a standard anti-inflammatory drug. Histological assessment showed colonic tissue damages in methionine treated group compared to control (P <0.001) as mucosal inflammation, crypt damage and hyperemia. Crude extract markedly reduces the damage induced by the Hhcy and preserved the histoarchitecture of colonic tissue compared to M group (P <0.001). This study provides clear evidence that Helix aspersa crude extract treatment decrease the major inflammatory markers homocysteine and CRP. Also it acts as a potent protective agent of colon against the inflammatory state induced by elevated level of plasma homocysteine.

Consumption of Cashew (Anacardium occidentale L.) Nuts Counteracts Oxidative Stress and Tissue Inflammation in Mild Hyperhomocysteinemia in Rats

“…It has also been widely reported that HHcy induces acute and chronic inflammatory events via NF-κB regulation [ 12 ]. In a neuroblastoma cell line, an induced high level of Hcy was demonstrated to boost NF-kB levels, and this was inhibited by the introduction of antioxidants [ 58 ].…”

“…For histological analysis, tissues were subjected to hematoxylin and eosin staining and observed by competent pathologists using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy), associated with Leica LAS X Navigator software (Leica Microsystems SpA, Milan, Italy). Histological injuries were scored as previously reported [ 12 , 32 , 33 ]. Paraffin-embedded skin tissues with a thickness of 5 μm were stained with Masson’s trichrome, according to the manufacturer’s protocol (Bio-Optica, Milan, Italy) [ 34 , 35 ].…”

“…The influence of HHcy on organs’ oxidative state has been examined in diverse tissues such as the endothelium [ 8 ], liver [ 9 ], heart, and brain [ 10 , 11 ]. Hcy may also have pathogenetic implications in inflammatory bowel disease (IBD), demonstrating that it is a pro-inflammatory and immunostimulating molecule [ 12 ]. Hcy is thought to stimulate the generation of hydroxyl radicals, which leads to lipid peroxidation (LPO).…”