B7‐1 and B7‐2 act differentially in the induction of a T cell response: Their impact for a HLA‐matched and HLA‐mismatched anti‐tumor immunotherapy

Kronfeld, Kai; Abken, Hinrich; Seliger, Barbara

doi:10.1002/ijc.21230

Cited by 12 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…T cells were coincubated with paraformaldehyde-fixed wild-type (wt) MZ1257RC cells, B7-1-and B7-2-modified MZ1257RC cells, or MZ1257RC cells transfected with "empty" vector DNA. Cells of the B7-transduced MZ1257RC clones express about 1.25 ϫ 10 6 B7-1 and B7-2 molecules/cell, respectively (22). Upon coincubation with either B7-1 ϩ or B7-2 ϩ MZ1257RC cells T cells increased proliferation and cytokine secretion (interferon-␥, granulocyte macrophage colony-stimulating factor, tumor necrosis factor-␣, and IL-10), whereas coincubation with wt and mocktransfected MZ1257RC cells did not (data not shown).…”

Section: T Cells Express An Altered Pattern Of Proteins Upon B7mentioning

confidence: 99%

“…MZ1257RC cells were equipped with B7-1 and B7-2, respectively, by DNA transfection as described previously (12,22). MZ1257RC cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) FCS, 2 mM L-glutamine, 10 IU/ml penicillin, 100 g/ml streptomycin, 10 mM sodium pyruvate, 100 mM Hepes, and 1ϫ non-essential amino acids.…”

Section: Isolation and Stimulation Of T Cells-peripheral Blood Mononumentioning

confidence: 99%

See 1 more Smart Citation

B7/CD28 Costimulation of T Cells Induces a Distinct Proteome Pattern

Kronfeld

Hochleitner

Mendler

et al. 2005

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

The best studied costimulatory pathway is represented by the CD28/CTLA4-B7 system (2, 3) where B7-1 (CD80) and B7-2 (CD86) bind either to an activating (CD28) or to an inhibitory (CTLA4) receptor on T cells (4). A variety of studies demonstrate the significance of CD28 costimulation in the clonal expansion of naïve T cells, the regulation of long term survival of T cells, induction of cell cycle progression, and cytokine secretion (5). The elucidation of the CD28-mediated functions has facilitated the development of therapeutic strategies, particularly vaccination studies, that target this pathway. Most efforts to induce antigen-specific immunity by application of cancer vaccines have focused on the generation of tumor-reactive CD8 ϩ cytotoxic T cells (CTLs) (6). Whereas B7-negative tumor cells most often fail to induce an efficient immune response, B7-1 and/or B7-2 expression on tumor cells augments the anti-tumor response by inducing T cell proliferation, cytokine secretion, and an enhanced generation of tumor-specific CD8 ϩ CTLs (7-10). B7-transduced renal cell carcinoma cells are an example of the strategy to stimulate tumor-infiltrating T cells to promote an efficient antitumor response (11,12).The efficacy of an antitumor reaction is usually monitored by in vitro determination of the number of antigen-specific T cells that secrete cytokines in response to antigen challenge. The monitoring can be achieved by enzyme-linked immunospot techniques (13,14), flow cytometry (15), multiplex-based immunoassays (16) or . Such assays, however, do not provide any information regarding the various signaling and metabolic pathways activated upon CD28 costimulation of the T cell. Particularly only limited information on the genetic expression programs linked to CD28 costimulation exists (18 -20), whereas to our best knowledge no study to date has assessed the protein expression patterns of both prestimulated and naïve T cells upon CD28 costimulation. To address the complexity of the T cell response directed against a defined, B7-transduced tumor cell vaccine, here we applied proteome-based technologies and identified a variety of differentially expressed proteins in T cells following B7-1 and

show abstract

Section: T Cells Express An Altered Pattern Of Proteins Upon B7mentioning

confidence: 99%

Section: Isolation and Stimulation Of T Cells-peripheral Blood Mononumentioning

confidence: 99%

B7/CD28 Costimulation of T Cells Induces a Distinct Proteome Pattern

Kronfeld

Hochleitner

Mendler

et al. 2005

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the other hand, upon subcutaneous administration without adjuvant, ZPS activates the APCs differentially than during intra-abdominal challenge, or activate a different subset of APCs than those involved in ZPS presentation in the peritoneal cavity, resulting in the activation of regulatory T cells. We and others previously reported that ZPS can induce the upregulation of CD86 on APCs [28, 41] in vitro and signaling through CD86 is reported in promoting the generation of IL-10-producing T cells [77, 78]. Thus, it will be interesting to know if the differences that occur in the immune responses when ZPS is administered by intraperitoneal or subcutaneous routes are due to differences in the presentation of ZPS antigens by the APCs at these sites.…”

Section: Zps Modulates Cytokines Of the Immune Systemmentioning

confidence: 99%

The Modulation of Adaptive Immune Responses by Bacterial Zwitterionic Polysaccharides

Stephen

Groneck

Kalka-Moll

2010

International Journal of Microbiology

View full text Add to dashboard Cite

The detection of pathogen-derived molecules as foreign particles by adaptive immune cells triggers T and B lymphocytes to mount protective cellular and humoral responses, respectively. Recent immunological advances elucidated that proteins and some lipids are the principle biological molecules that induce protective T cell responses during microbial infections. Polysaccharides are important components of microbial pathogens and many vaccines. However, research concerning the activation of the adaptive immune system by polysaccharides gained interest only recently. Traditionally, polysaccharides were considered to be T cell-independent antigens that did not directly activate T cells or induce protective immune responses. Here, we review several recent advances in “carbohydrate immunobiology”. A group of bacterial polysaccharides that are known as “zwitterionic polysaccharides (ZPSs)” were recently identified as potent immune modulators. The immunomodulatory effect of ZPSs required antigen processing and presentation by antigen presenting cells, the activation of CD4 T cells and subpopulations of CD8 T cells and the modulation of host cytokine responses. In this review, we also discuss the potential use of these unique immunomodulatory ZPSs in new vaccination strategies against chronic inflammatory conditions, autoimmunity, infectious diseases, allergies and asthmatic conditions.

show abstract

“…These tumors have been shown to express T cell inhibitory molecules such as B7-H1 and ICOSL to engage their inhibitory counterparts on the T cell surface (Figure 2(a)). On the contrary, tumors that are induced to express costimulatory molecules that activate T cells, such as B7.1 (also known as CD80), and B7.2 (also known as CD86) are cleared by the immune system and thus may be used as a strategy to promote tumor immunity [148, 149]. …”

Section: Costimulation and Modulatory Molecules At The Immunologicmentioning

confidence: 99%

Modulation of Tumor Immunity by Soluble and Membrane-Bound Molecules at the Immunological Synapse

González

Carreño

Céspedes

et al. 2013

Clinical and Developmental Immunology

View full text Add to dashboard Cite

To circumvent pathology caused by infectious microbes and tumor growth, the host immune system must constantly clear harmful microorganisms and potentially malignant transformed cells. This task is accomplished in part by T-cells, which can directly kill infected or tumorigenic cells. A crucial event determining the recognition and elimination of detrimental cells is antigen recognition by the T cell receptor (TCR) expressed on the surface of T cells. Upon binding of the TCR to cognate peptide-MHC complexes presented on the surface of antigen presenting cells (APCs), a specialized supramolecular structure known as the immunological synapse (IS) assembles at the T cell-APC interface. Such a structure involves massive redistribution of membrane proteins, including TCR/pMHC complexes, modulatory receptor pairs, and adhesion molecules. Furthermore, assembly of the immunological synapse leads to intracellular events that modulate and define the magnitude and characteristics of the T cell response. Here, we discuss recent literature on the regulation and assembly of IS and the mechanisms evolved by tumors to modulate its function to escape T cell cytotoxicity, as well as novel strategies targeting the IS for therapy.

show abstract

B7‐1 and B7‐2 act differentially in the induction of a T cell response: Their impact for a HLA‐matched and HLA‐mismatched anti‐tumor immunotherapy

Cited by 12 publications

References 26 publications

B7/CD28 Costimulation of T Cells Induces a Distinct Proteome Pattern

B7/CD28 Costimulation of T Cells Induces a Distinct Proteome Pattern

The Modulation of Adaptive Immune Responses by Bacterial Zwitterionic Polysaccharides

Modulation of Tumor Immunity by Soluble and Membrane-Bound Molecules at the Immunological Synapse

Contact Info

Product

Resources

About