B3GNT5 is a novel marker correlated with stem‐like phenotype and poor clinical outcome in human gliomas

Jeong, Hang Yeon; Park, Seo-Young; Kim, Hyun‐Jin; Moon, Seong Yong; Lee, Seongsoo; Lee, Seung Ho; Kim, Sung-Hak

doi:10.1111/cns.13439

Cited by 15 publications

(18 citation statements)

References 20 publications

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“…Functionally, the overexpression of B3GNT5 relieved the sh-2HG-1-induced inhibition of cell proliferation, migration and invasion. These results were consistent with the previous study in human glioma in which B3GNT5 was revealed to act as a tumor promoter ( 35 ). However, a major limitation of the current study is that B3GNT5-related signaling pathways and the effect of MIR4435-2HG in liver cancer in vivo were not comprehensively studied and thus will be explored further in the future.…”

Section: Discussionsupporting

confidence: 93%

lncRNA MIR4435‑2HG promotes the progression of liver cancer by upregulating B3GNT5 expression

Zhu

et al. 2021

Mol Med Rep

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Several studies have indicated that dysregulation of long non-coding RNAs (lncRNAs) participates in the initiation and progression of cancer. The lncRNA MIR4435-2HG was previously reported to act as an oncogene in human cancer, including liver cancer. However, its role in the pathogenesis in liver cancer is largely unclear. The present study aimed to reveal the molecular mechanism by which MIR4435-2HG regulates liver cancer. The expression levels of MIR4435-2HG in liver cancer and adjacent normal tissues were analyzed using The Cancer Genome Atlas database. MIR4435-2HG expression was validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in cancer cells in vitro . The target genes of MIR4435-2HG were predicted using bioinformatics analysis. Interactions between miR-136-5p, MIR4435-2HG and B3GNT5 were detected using luciferase reporter assays, and their effects on cell viability, migration and invasion were assessed using Cell Counting Kit-8, wound healing and Transwell assays. The effects of miR-136-5p and MIR4435-2HG on B3GNT5 expression were confirmed by western blot analysis. The results revealed that MIR4435-2HG expression was upregulated in primary liver cancer and liver cancer cell lines, and was positively associated with advanced tumor stage, metastasis and poor prognosis in patients with liver cancer. Knockdown of MIR4435-2HG significantly inhibited the proliferation, migration and invasion of liver cancer cells. Furthermore, miR-136-5p was determined to be a direct target of MIR4435-2HG and suppressed MIR4435-2HG expression by binding with the seed region of the 3′-UTR of MIR4435-2HG in liver cancer cells. Functional studies showed that the inhibitory effects of MIR4435-2HG knockdown on cell proliferation, migration and invasion were significantly rescued by inhibiting miR-136-5p. Furthermore, the target gene, B3GNT5, of miR-136-5p was confirmed by bioinformatics analysis and RT-qPCR. In addition, B3GNT5 expression was regulated by the MIR4435-2HG / miR-136-5p axis. In conclusion, the present study indicated that MIR4435-2HG facilitated the progression of liver cancer via the MIR4435-2HG / miR-136-5p / B3GNT5 axis, which demonstrated that MIR4435-2HG may be a potential biomarker for the prognosis and treatment of liver cancer.

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Section: Discussionsupporting

confidence: 93%

lncRNA MIR4435‑2HG promotes the progression of liver cancer by upregulating B3GNT5 expression

Zhu

et al. 2021

Mol Med Rep

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“…This proves that the 11 key genes we found are meaningful for clinical prognosis of glioma. Among the 11 genes, 7 of them (i.e., B3GNT5, BICD1, KIF3A, HAUS1, MTMR3, ITGB3 and EXTL3) have been confirmed to be associated with the prognosis of glioma (Kim et al, 2011;Sumazin et al, 2011;Huang et al, 2017;Zhou et al, 2018;Jeong et al, 2020;Li et al, 2020;Wang et al, 2020). Our findings imply that the functions of these genes in glioma prognosis may be related to the methylation regulation of their AS events.…”

Section: Influence Of the Me-sqtl Genes On Clinical Prognosis Of Gliomamentioning

confidence: 53%

Genome-Wide Analysis for the Regulation of Gene Alternative Splicing by DNA Methylation Level in Glioma and its Prognostic Implications

Yang

Wang

et al. 2022

Front. Genet.

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Glioma is a primary high malignant intracranial tumor with poorly understood molecular mechanisms. Previous studies found that both DNA methylation modification and gene alternative splicing (AS) play a key role in tumorigenesis of glioma, and there is an obvious regulatory relationship between them. However, to date, no comprehensive study has been performed to analyze the influence of DNA methylation level on gene AS in glioma on a genome-wide scale. Here, we performed this study by integrating DNA methylation, gene expression, AS, disease risk methylation at position, and clinical data from 537 low-grade glioma (LGG) and glioblastoma (GBM) individuals. We first conducted a differential analysis of AS events and DNA methylation positions between LGG and GBM subjects, respectively. Then, we evaluated the influence of differential methylation positions on differential AS events. Further, Fisher’s exact test was used to verify our findings and identify potential key genes in glioma. Finally, we performed a series of analyses to investigate influence of these genes on the clinical prognosis of glioma. In total, we identified 130 glioma-related genes whose AS significantly affected by DNA methylation level. Eleven of them play an important role in glioma prognosis. In short, these results will help to better understand the pathogenesis of glioma.

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“…ACSL1 exists in cells of the liver, heart and fat, as is considered to affect activation fatty acid synthesis of triglycerides through the peroxisome proliferator-activated receptor γ pathway (44). B3GNT5 has been suggested as the key glycosyltransferase in the biosynthesis of the (neo-)lacto series of glycosphingolipid in regulating malignancy of glioblastoma multiforme (45). This result implies that the etiology of AMI is associated with genes related to intravascular disease, immune response and brain-derived factor regulation system, suggesting that ACSL1, B3GNT5 and MGAM may be also used as biomarkers for gene expression in early AMI.…”

Section: Discussionmentioning

confidence: 99%

Potential biomarkers of acute myocardial infarction based on co‑expression network analysis

Liu

et al. 2021

Exp Ther Med

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Acute myocardial infarction (AMI) is a common cause of death in numerous countries. Understanding the molecular mechanisms of the disease and analyzing potential biomarkers of AMI is crucial. However, specific diagnostic biomarkers have thus far not been fully established and candidate regulatory targets for AMI remain to be determined. In the present study, the AMI gene chip dataset GSE48060 comprising blood samples from control subjects with normal cardiac function (n=21) and patients with AMI (n=26) was downloaded from Gene Expression Omnibus. The differentially expressed genes (DEGs) between the AMI and control groups were identified with the online tool GEO2R. The co-expression network of DEGs was analyzed by calculating the Pearson correlation coefficient of all gene pairs, mutual rank screening and cutoff threshold screening. Subsequently, the Gene Ontology (GO) database was used to analyze the genes' functions and pathway enrichment of genes in the most important modules was performed. Kyoto Encyclopedia of Genes and Genomes (KEGG) Disease and BioCyc were used to analyze the hub genes in the module to determine important sub-pathways. In addition, the expression of hub genes was confirmed by reverse transcription-quantitative PCR in AMI and control specimens. In the present study, 52 DEGs, including 26 upregulated and 26 downregulated genes, were identified. As key hub genes, three upregulated genes (AKR1C3, RPS24 and P2RY12) and three downregulated genes (ACSL1, B3GNT5 and MGAM) were identified from the co-expression network. Furthermore, GO enrichment analysis of all AMI co-expression network genes revealed functional enrichment mainly in 'RAGE receptor binding' and 'negative regulation of T cell cytokine production'. In addition, KEGG Disease and BioCyc analysis indicated functional enrichment of the genes RPS24 and P2RY12 in 'cardiovascular diseases', of AKR1C3 in 'cardenolide biosynthesis', of MGAM in 'glycogenolysis', of B3GNT5 in 'glycosphingolipid biosynthesis' and of ACSL1 in 'icosapentaenoate biosynthesis II'. In conclusion, the hub genes AKR1C3, RPS24, P2RY12, ACSL1, B3GNT5 and MGAM are potential markers of AMI, and have potential application value in the diagnosis of AMI.

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B3GNT5 is a novel marker correlated with stem‐like phenotype and poor clinical outcome in human gliomas

Cited by 15 publications

References 20 publications

lncRNA MIR4435‑2HG promotes the progression of liver cancer by upregulating B3GNT5 expression

lncRNA MIR4435‑2HG promotes the progression of liver cancer by upregulating B3GNT5 expression

Genome-Wide Analysis for the Regulation of Gene Alternative Splicing by DNA Methylation Level in Glioma and its Prognostic Implications

Potential biomarkers of acute myocardial infarction based on co‑expression network analysis

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