B220 is Expressed on Apoptotic Thymocytes Induced by X-Irradiation.

Oka, Shuntaro; Kubo, Kihei; Matsuyama, Satoshi; Takamori, Yasuhiko

doi:10.1292/jvms.61.337

Cited by 3 publications

(6 citation statements)

References 18 publications

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“…41) Recently, it has been reported that B220 was expressed on apoptotic T cells. [42][43][44] In this study, we consider that B220 ϩ cell is a B cell, because the CD3 ϩ…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Antibody Production by Acetaminophen Independent of Liver Injury in Mice.

Yamaura

Ogawa

Yonekawa

et al. 2002

Biological & Pharmaceutical Bulletin

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The causal relationship between the inhibition of antibody production and liver injury induced by single doses of acetaminophen (APAP) was investigated in mice. The liver injury and antibody production were evaluated using the serum transaminase activity and the number of antibody forming cells against sheep red blood cells (SRBC), respectively. The relevance of APAP hepatotoxicity with inhibiting antibody production was elucidated in fasted and fed mice treated with a single oral administration of APAP. In fasted mice, the oral administration of APAP produced serious liver injury, while it was not the case in the fed mice. As the antibody production was measured under these conditions, APAP significantly depressed the antibody production in fed mice as well as in fasted mice. The rate of B220 positive cells in the splenocytes was significantly decreased by APAP administration in both the fasted and fed mice. Splenocytes proliferative responses following mitogenic stimulation with concanavalin A or lipopolysaccharide were inhibited by APAP. Moreover, APAP added directly to the splenocyte culture also inhibited the in vitro antibody-producing response to SRBC. These findings indicate that the APAP-induced depression of antibody production may not be a secondary response to APAP-hepatitis, but may be a primary response to APAP.

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“…41) Recently, it has been reported that B220 was expressed on apoptotic T cells. [42][43][44] In this study, we consider that B220 ϩ cell is a B cell, because the CD3 ϩ…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Antibody Production by Acetaminophen Independent of Liver Injury in Mice.

Yamaura

Ogawa

Yonekawa

et al. 2002

Biological & Pharmaceutical Bulletin

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“…The dramatical reduction of CD4 + 8 + can be attributed to apoptosis within 24 hr after irradiation, when the expression of B220 increased on the subset [13]. Most of CD4 + 8 -and CD4 -8 + cells survived exposure to 6.8 Gy were positive for CD3 and LECAM-1.…”

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confidence: 99%

“…We have previously reported that B220, a specific marker of pan-B cells, was expressed on both pre-apoptotic and apoptotic thymocytes of DBA1 mice early after whole-body X-irradiation [13]. However, the behavior of thymocyte subsets in the early radiation-induced apoptotic process has not been well known.…”

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confidence: 99%

“…Animals: DBA1 mice were originally provided by Dr. J. Hayakawa (Institute for Experimental Animals, School of Medicine, Kanazawa University, Kanazawa, Japan) and have been bred at our laboratory [13].…”

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confidence: 99%

“…We have previously observed that a dramatic reduction of the cell number accompanied some cellular changes characteristic for apoptosis, namely, decrease in cell size as well as DNA content per cell and the expression of B220, an isoform of murine CD45 molecule [13]. Therefore, the cause of reduction in radiosensitive cells can be ascribed to radiation-induced apoptosis.…”

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confidence: 99%

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Flow Cytometric Analysis of Thymocyte Subpopulations in Mice after Whole-Body X-Irradiation.

Oka

Kubo

Matsuyama

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. To determined the cellular kinetics of thymocyte subpopulations in DBA1 mice after whole-body 6.8 Gy X-irradiation, they were analyzed for the expression of several cell surface antigens using flow cytometry. The results show that i) The majority of thymocytes rapidly depleted by irradiation was CD4 + 8 + cells. ii) radioresistant CD4 + 8 -and CD4 -8 + survived 18-48 hr after X-irradiation were considered to be relatively mature type, since they expressed high levels of CD3 and LECAM-1. iii) CD3-positive cells were detected in CD4 -8 -cells at 72 hr after irradiation.-KEY WORDS: cell surface antigen, murine thymocyte, X-irradiation.

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Presence of B220 within thymocytes and its expression on the cell surface during apoptosis

Oka

Mori

Matsuyama³

et al. 2000

Immunology

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SUMMARYB220 is the full-length splicing isoform of a tyrosine phosphatase CD45 and is predominantly expressed as a transmembrane protein on B cells. Other splicing isoforms of CD45 are yielded by alternative splicing of exons 4, 5 and 6. Recently, the expression of B220 on peripheral T cells during activation-induced cell death has been reported. To investigate whether B220 is implicated in apoptosis of immature T cells, we analysed (by¯ow cytometry using the anti-B220 monoclonal antibody, RA3-6B2) the expression of B220 on mouse thymocytes undergoing X-irradiation-and dexamethasone (DEX)-induced apoptosis. The expression of B220 on thymocytes positive for Thy-1 was induced by X-irradiation or DEX treatment and increased with length of incubation. The expression of B220 was pronounced on the apoptotic hypodiploid cells in the fraction showing lower forward scattering values. Reverse transcription±polymerase chain reaction detected mRNA containing exons 4, 5 and 6 of CD45 in normal thymocytes as well as those exposed to X-rays or DEX. Surprisingly, cytoplasmic B220 antigens were detected in a considerable fraction of normal thymocytes. Moreover, the expression level of the 220 000-MW protein in normal thymocytes was similar to that in the thymocytes undergoing apoptosis. During apoptosis, the expression level of B220 antigen was reduced in the cytoplasm but, conversely, up-regulated on the surface of thymocytes. These results suggest that B220 is constitutively expressed as a cytoplasmic form within thymocytes and possibly translocated to the cell membrane during apoptosis.

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B220 is Expressed on Apoptotic Thymocytes Induced by X-Irradiation.

Cited by 3 publications

References 18 publications

Inhibition of the Antibody Production by Acetaminophen Independent of Liver Injury in Mice.

Inhibition of the Antibody Production by Acetaminophen Independent of Liver Injury in Mice.

Flow Cytometric Analysis of Thymocyte Subpopulations in Mice after Whole-Body X-Irradiation.

Presence of B220 within thymocytes and its expression on the cell surface during apoptosis

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