Transplantation of a human ovarian cystadenocarcinoma into severe combined immunodeficient (SCID) mice — formation of metastases without significant alteration of the tumour cell phenotype

Abstract

Human ovarian papillary cystadenocarcinoma cells were injected intraperitoneally into severe combined immunodeficient (SCID) mice. After intraperitoneal application the cells, designated SoTü, grew well in vivo, lodged on to the peritoneum, formed local metastatic deposits, led to the development of ascites in the mice and formed distant metastases in the lungs. If lodged in the ovary, the morphology of the SoTü tumour remarkably resembled that of the primary tumour in the patient. In contrast, several attempts failed to maintain the SoTü cells in vitro. If SCID mouse ascites derived SoTü were transplanted subcutaneously in SCID mice, they formed cystic tumours which also metastasized into the lungs. Immunophenotypical analysis of cell adhesion molecule expression, cell proliferation markers, various oncoproteins, keratin, vimentin, and lectin binding site expression all showed striking similarity between the primary tumour and the SCID mouse explants. In particular, expression of binding sites for the lectin Helix pomatia agglutinin (HPA), which has been shown to be an index of metastatic potential in several human carcinomas, was found on the primary tumour as well as on tumour cells grown in SCID mice, indicating that HPA might be a prognostic indicator in ovarian carcinoma as well. Our results demonstrate that the human/SCID mouse system can mimic growth and distant metastasis formation of human ovarian carcinoma. Although the formation of distant metastases is a relatively rare event in patients, this model system might help to elucidate mechanisms of metastasis formation in ovarian cancer.

Establishment of intraperitoneal transplantation model of cisplatin-resistant ovarian carcinoma cell in scid mice

“…There are a few of types of models based on the transplantation routes mainly including subcutaneous transplantation, orthotopic transplantation and abdominal transplantation. Silver and Schumacher, et al transplanted surgical specimen of ovarian carcinoma and precipitated cells of ascites to subcutaneous tissues of SCID mouse and succeeded in the establishment of subcutaneous tumor bearing model [6][7] . Similar report was also given by Cui Heng, et al (from Beijing [7] ) [8] .…”

“…Silver and Schumacher, et al transplanted surgical specimen of ovarian carcinoma and precipitated cells of ascites to subcutaneous tissues of SCID mouse and succeeded in the establishment of subcutaneous tumor bearing model [6][7] . Similar report was also given by Cui Heng, et al (from Beijing [7] ) [8] . The growth of tumor can be observed conveniently because the early nodules formed from the tumor can be touched easily [9] .…”

“…Walker [11] and Schumashe [7] , et al transplanted human ovarian carcinoma cell strain or precipitated cells in ascites into SCID mouse body and the tumor grew well.…”

Expression of N-Acetyl Galactosaminylated and Sialylated Glycans by Metastases Arising from Primary Breast Cancer

“…Reports of HPA binding characteristics of inoculum, primary tumour and metastases in a SCID mouse model suggest that in this system they remain unchanged [15][16][17], but tumours were harvested soon after metastases had established and nothing is known of longterm glycosylation changes with disease progression. In this study, HPA negative subpopulations were noted in otherwise strongly HPA positive metastases, which may be consistent with HPA-positive tumours developing HPA negative clones and gradually losing HPA binding characteristics over time.…”

In vivoanti-tumor effect of hybrid vaccine of dendritic cells and esophageal carcinoma cells on esophageal carcinoma cell line 109 in mice with severe combined immune defi ciency

“…Human lymphocytes to be transferred in SCID mice were isolated from the whole blood obtained from healthy adult volunteers as previously described [7][8][9][10][11] . Human peripheral blood lymphocytes (PBL) were isolated by Ficoll-Paque gradient centrifugation under sterile conditions.…”