<b>Monoamineoxidase and cholinesterase activity in neurons and neuropil from the rat cerebral cortex</b>

Sinha, Arun K.; Rose, Steven

doi:10.1111/j.1471-4159.1972.tb05106.x

Cited by 14 publications

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“…It is believed that transmitters [e.g., catecholmines and y-aminobutyric acid (GABA)] released at the synaptic cleft in the central nervous system are inactivated by neuronal and extraneuronal cellular uptake and storage followed by intracellular enzymatic catabolism (for review see McGeer et al,197&z,b). Astroglial cells have the capacity to accumulate and metabolize neurotransmitters (Silberstein et al, 1972; Sinha and Rose, 1972;Murphy et al, 1976;Sellstrom et al, 1975;Schousboe et al, 1977; Bardakdjian et al, 1979;Pelton et al, 1981).…”

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“…Monoamine oxidase (MAO), the enzyme that deaminates catecholamines, is present in mitochondria in different regions of the brain. M A 0 has been demonstrated in bulk-isolated glial cells (Hamberger et al, 1970;Sinha and Rose, 1972;Hazama et al, 1976), as well as in cultured astrocytoma cells (Silberstein et al, 1972) and in astrocytes in primary culture (Pelton et al, 1981). Catechol-O-methyltransferase (COMT) catalyzes the transfer of methyl groups to substrates containing catechol groups in the presence of S-adenosylmethionine (SAM) as methyl donor and magnesium ions as activator (Guldberg, 1979).…”

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MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures

Hanssoń

Sellström

1983

Journal of Neurochemistry

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Cultures from cerebral hemispheres of newborn rats contain the enzymes monoamine oxidase (MAO), catechol-O-methyltransferase (COMT), and gamma-aminobutyric acid alpha-ketoglutarate transaminase (GABA-T). The COMT activity was higher in the cultures than in adult rat cerebral hemispheres. The MAO activity was comparable in the cultures and in the rat cerebral hemispheres. The activities of both these enzymes increased with age in the cultures and in the rat brain hemispheres. In the culture the activities were further potentiated by removal of fetal calf serum and addition of 0.1 mM dibutyryl-cyclic AMP (dB-cAMP). GABA-T activity was, however, lower in the cultures than in the adult rat brain hemispheres. The activity increased in brain during postnatal maturation. No changes in the enzyme activity were observed in the cultures, either during growth or after removal of fetal calf serum and addition of dB-cAMP.

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MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures

Hanssoń

Sellström

1983

Journal of Neurochemistry

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Enzymology of monoamine oxidase

Tipton

1986

Cell Biochemistry & Function

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Cellular compartmentation of brain metabolism and its functional significance

Rose

1975

J of Neuroscience Research

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This paper reviews work from our laboratory on the metabolism and interrelations of isolated neuronal and neuropil fractions. The cell preparations are evaluated according to criteria of yield, purity, and integrity. Differences in the levels of activity of six groups of enzymes, of glucose metabolism, amino-acid metabolism, transmitter metabolism, acid hydrolysis, alkaline hydrolysis, and carbonic anhydrase have been followed, and neuronal and glial marker enzymes are proposed. Lysosomes and their enzymes are concentrated in neuronal perikarya. Metabolically, although no major differences in glucose oxidation have been found, there is considerable evidence of compartmentation of amino acids and their metabolism. At short times after injection of 3H-lysine as precursor in vivo, neuronal incorporation is high as compared with neuropil; at longer times the ratio is reversed, and we interpret this as evidence for the presence of a rapidly labeling protein fraction present in the neuromal perikarya but subsequently transported out. Neuronal protein incorporation is suppressed in the visual but not the motor cortex of dark-reared rats and is switched on following exposure to light; there is evidence that the suppressed fraction of neuronal protein synthesis includes the rapidly labeling component. A model for neuronal-glial metabolic interaction and its state-dependence in response to changes in the organism's environment and behavior is sketched out.

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Monoamineoxidase and cholinesterase activity in neurons and neuropil from the rat cerebral cortex

Cited by 14 publications

References 1 publication

MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures

MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures

Enzymology of monoamine oxidase

Cellular compartmentation of brain metabolism and its functional significance

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