Molecular cloning of the feline CD8β‐chain

Abstract

Human, mouse and rat CD8 have been described as being disulphide‐linked heterodimers of α and β chains. More recently the chicken α and β chains were described. In the bovine and feline immune system only the α‐chain was reported. In this study we have cloned and determined the nucleotide sequence of a cDNA encoding the β‐chain of the feline T‐cell surface antigen CD8. Using a nested polymerase chain reaction (PCR) and two primer pairs designed from the human CD8β cDNA nucleotide sequence, we amplified a 430 base pair fragment from a feline thymus cDNA library which was used as a probe for screening the feline library at high stringency. After three rounds of screening, five clones were isolated. A clone, named FTb‐6, containing a 3.8 kilobase pair insert was mapped, sequenced and compared with the published sequences of the genes encoding the human, mouse, rat and avian CD8β. We have determined the primary structure of the feline CD8β. The feline CD8β has an open reading frame, 630 nucleotides in length encoding a protein with 210 amino acid residues and its composition showed that the feline molecule is a member of the immunoglobulin gene super family.

Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine

“…Human CD3e ERPPPVPNPDYEP Feline CD3e EKPPPVPNPDYEP The speci®city of CD8a and CD8b mAbs for each CD8 chain was con®rmed by screening each mAb against the appropriately transfected feline recombinant CD8 constructs (Pecoraro et al, 1994;Pecoraro et al, 1996) (M. Shimojima, personal communication). The putative CD49d reagent is designated CD49d-like based on immunoprecipitation data and appropriate cell and tissue distribution (Woo and Moore, unpublished data).…”

The Role of Polymerase Chain Reaction and Its Newer Developments in Feline Medicine

“…A few developments may be of direct interest for veterinary clinicians and are therefore mentioned here. Many of the feline cytokines and other molecules of importance in feline medicine have now been sequenced (McGraw et al 1990, Cozzi et al 1993, Ohashi et al 1993, Gallagher et al 1995, Mayr et al 1995, Rimstad et al 1995, Dunham & Onions 1996, Pecoraro et al 1996, Dean et al 1998, Lutz & Leutenegger 1998). If sequences are known, they can readily be used for the quantitation of mRNA expression as it was the case for IL-2, IFN gamma, IL-4, IL-6, IL-10, IL-12 and IL-16 (Rottman et al 1995, Dean et al 1998, Fehr et al 1997, Leutenegger et al 1998b).…”