B‐Lymphoproliferative disorders: A Proposed unified pathogenetic pathway

Ezdinli, Ediz Z.; Nanus, David M.

doi:10.1002/hon.2900010403

Cited by 5 publications

References 104 publications

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5′ nucleotidase in chronic b cell leukemias: A cytochemical and ultrastructural study

et al. 1990

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We studied by cytochemical means the distribution of 5' nucleotidase (5' NT), a purine degradative enzyme, in the circulating lymphocytes of 24 healthy donors and 41 cases of chronic lymphoid leukemias, classified according to morphological and immunological criteria. About half the normal circulating lymphocytes were 5'NT positive and exhibited variable degrees of enzyme activity. Among chronic B lymphocytic leukemias we found high percentages of positive cells only in the phenotypically more mature cases. Moreover all cases of hairy cell, follicular cell, lymphoplasmacytic, and plasma cell leukemia showed moderate or weak 5' NT reactivity. Also one case of chronic T lymphocytic leukemia, CD8 positive, was moderately positive, while another, with large granular lymphocyte morphology, was completely negative. Electron microscopy revealed a discontinuous, granular plasma membrane reaction pattern, varying in intensity from case to case. In conclusion, our results confirm the usefulness of the 5' NT cytochemical reaction for identification of lymphoid populations at different stages of maturation in chronic B cell disorders.

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5′ nucleotidase in chronic b cell leukemias: A cytochemical and ultrastructural study

et al. 1990

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Purine degradative enzymes in circulating malignant cells of patients with chronic B cell neoplasia

Knauf

Ganeshaguru

et al. 1987

Hematological Oncology

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Previous reports have shown that the purine degradative enzymes adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and ecto-5'-nucleotidase (5'NT), play an important role in the normal development of lymphocytes and that investigations of these enzymes are of value in defining subsets of lymphoid malignancies of T-cell origin. Pharmacological inhibition of one of these enzymes has been found to be an effective treatment for a few lymphatic neoplasia. We have studied the activities of the above enzymes in the circulating malignant cells of 25 patients with B-chronic lymphatic leukemia (B-CLL), four patients with B prolymphocytic leukemia (PLL), seven patients with leukemic centrocytic lymphoma (CC), 18 patients with hairy cell leukemia (HCL) and 16 patients with immunocytoma (IC). For comparison, the blasts of nine patients with 'common' acute lymphatic leukemia (cALL) and normal T (n = 12) and B (n = 8) cells were simultaneously investigated. Despite morphologic similarity, the leukemic cells of the chronic B cell malignancies demonstrate different enzyme patterns. B-CLL is characterized by very low activities of all the enzymes ADA, PNP and 5'NT. In the cells of HCL the highest values of PNP are found. The leukemic cells of IC are characterized by low levels of ADA but moderate levels of PNP and high levels of 5'NT. Thus some of the entities of B malignancies show typical enzyme patterns which might be of importance in defining maturation stages of the disease. The differences in these enzyme patterns can also be made use of in therapy with enzyme inhibitors such as deoxycoformycin.

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B Cell Leukaemias

Forbes

1987

Essential Oncology of the Lymphocyte

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B‐Lymphoproliferative disorders: A Proposed unified pathogenetic pathway

Cited by 5 publications

References 104 publications

5′ nucleotidase in chronic b cell leukemias: A cytochemical and ultrastructural study

5′ nucleotidase in chronic b cell leukemias: A cytochemical and ultrastructural study

Purine degradative enzymes in circulating malignant cells of patients with chronic B cell neoplasia

B Cell Leukaemias

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