B-lymphoma cells process and present their endogenous immunoglobulin to major histocompatibility complex-restricted T cells.

Abstract

Antigen-presenting B-lymphoma cells were transfected with the gene encoding the immunoglobulin A2 light chain of MOPC315 cells (A2315). The A2 chain is expressed on the cell surface of the transfectants together with the endogenous heavy chain. (4) and that this recognition was under H-2-linked immune response (Ir) gene control (5). The hypothesis has been supported and extended by later work (6-8). Similar ideas have recently been proposed by others (9, 10), and MHC-restricted T cells specific for idiotypes (11,12) and allotypes (13) have been described. However, it is still unclear whether MHC-restricted T cells recognize processed forms of immunoglobulin exclusively and whether B cells can process their own immunoglobulin.To test this directly we have used recently established T-cell clones specific for an idiotope of A2315 in the context of the I-Ed MHC antigen (7,8). These clones, like the T cells studied in vivo before (4, 5), recognize an idiotypic determinant on A2315 around amino acid positions 94, 95, and 96 (7, 8

Antibody‐mediated delivery of antigen to chemokine receptors on antigen‐presenting cells results in enhanced CD4+ T cell responses

“…Previous reports have established that Ab must be internalized and processed before Ab-derived peptides can be presented [4][5][6]17]. Indeed, we demonstrate that T cell activation was abolished if the APC were treated with leupeptin or brefeldin A prior to incubation with CCR1-specific mAb.…”

“…However, the experiment can be performed using mAb that specifically target each chemokine receptor. It is well established that Ab are taken up by APC, processed, and presented on MHC class II molecules to CD4 + T cells [4,5]. Moreover, the presentation is further enhanced if the antibodies bind to surface molecules that intersect the endocytic processing pathway, such as transferrin receptor [6] or DEC-205 [7].…”

A peptide derived from an autoantibody can stimulate t cells in the (nzb × nzw)f1 mouse model of systemic lupus erythematosus

“…B cells can serve as APC (10,ll); they can process surface monomeric immunoglobulin and present peptides derived from it in their surface class I1 MHC molecules (12)(13)(14).…”

Review: To What Extent are T Cells Tolerant to Immunoglobulin Variable Regions?

“…It was further demonstrated that B cells continuously processed their own BCR and constitutively presented Id-peptides on their MHC class II molecules [9][10][11] [12]. Thus, B cells express two types of Id: (1) conformational Id determinants expressed on intact BCR, serologically detected by anti-Id antibodies and (2) short Id-peptides that are presented on MHC class II molecules to Id-specific T cells [9]. Id-peptide ⁄ MHC II presentation to CD4 + T cells represented at that time a novel type of interaction between T and B cells in the absence of external antigen, and it was suggested that such Id-driven T-B collaboration could play a role in immune regulation, autoimmunity and memory [13].…”

“…In the 1980s it became clear that immunoglobulins are processed and presented by antigen-presenting cells (APC) and that V-region-derived Id-peptides are displayed on MHC class II molecules to Id-specific CD4 + T cells [5][6][7][8]. It was further demonstrated that B cells continuously processed their own BCR and constitutively presented Id-peptides on their MHC class II molecules [9][10][11] [12]. Thus, B cells express two types of Id: (1) conformational Id determinants expressed on intact BCR, serologically detected by anti-Id antibodies and (2) short Id-peptides that are presented on MHC class II molecules to Id-specific T cells [9].…”