B-Lymphoid cell lines derived fromHLA-D homozygous donors

Hansen, John A.; Fu, Shu Man; Antonelli, Paolo; Kamoun, Malek; Hurley, John N.; Winchester, Robert; Dupont, Bo; Kunkel, Henry G.

doi:10.1007/bf01561413

Cited by 69 publications

(19 citation statements)

References 19 publications

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“…Monoclonal antibody SG465 recognizes a determinant present on DS molecules, which are molecules that are homologous by partial sequence analysis to murine I-A antigens (19). Cell lines were established from peripheral blood B lymphocytes of patients by Epstein-Barr virus transformation (20).…”

Section: Methodsmentioning

confidence: 99%

Specific HLA-DR4-associated histocompatibility molecules characterize patients with seropositive juvenile rheumatoid arthritis.

Nepom¹,

Nepom²,

Mickelson³

et al. 1984

J. Clin. Invest.

118

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A bstract. The structural and functional heterogeneity of HLA-DR4-associated specificities was investigated in patients with seropositive juvenile rheumatoid arthritis, a DR4-associated disease. Using a combination of HLA-D analysis by mixed lymphocyte culture and electrophoretic analysis of immunoprecipitated Ia molecules by two-dimensional polyacrylamide gels, we observed a surprisingly homogeneous pattern of HLA-D antigen expression. All patients expressed common structural products of the DR and DS loci, and 7/12 homozygous DR4 patients expressed a rare and subtle HLA-D heterozygous phenotype.

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Section: Methodsmentioning

confidence: 99%

Specific HLA-DR4-associated histocompatibility molecules characterize patients with seropositive juvenile rheumatoid arthritis.

Nepom¹,

Nepom²,

Mickelson³

et al. 1984

J. Clin. Invest.

118

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“…A preliminary report of this work has been presented (31 (33)(34)(35) and cell surface phenotype was defined by a panel of monoclonal antibodies specific for myelomonocytic differentiation antigens (10,11,14). The TdT' AML subgroup was composed of leukemias that would have been classified as typical FAB M I-M6 leukemias by conventional criteria (1), but which expressed the nuclear enzyme TdT (32 (29,40,41). On occasional instances in which control negative antisera gave reactions greater than a cytotoxicity score ofone, the microcytotoxicity data was not included for analysis.…”

Section: Introductionmentioning

confidence: 99%

Definition of a possible genetic basis for susceptibility to acute myelogenous leukemia associated with the presence of a polymorphic Ia epitope.

Seremetis¹,

Cuttner²,

Winchester³

1985

J. Clin. Invest.

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“…Established B-lymphoblastoid cell lines were available for some of the above HTC, otherwise new B-lymphoblastoid lines were prepared from cryopreserved HTC by cocultivation with Epstein-Barr virus (EBV) as described (21 (23). HLA-DR typing was performed on nylon wool purified peripheral blood B cells or B-lymphoblastoid cell lines as described (24, 25) using a panel of antisera recognizing HLA-DR1 through DRw1O.…”

mentioning

confidence: 99%

Electrophoretic analysis of human HLA-DR antigens from HLA-DR4 homozygous cell lines: correlation between beta-chain diversity and HLA-D.

Nepom¹,

Nepom²,

Mickelson³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

119

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Two-dimensional gel electrophoresis of immunoprecipitated human HLA-DR antigens from cells expressing the HLA-DR4 haplotype shows distinct clustering of (3-chain patterns. Six unique electrophoretic variants were observed among 17 HLA-DR4 homozygous cell lines (HCL) analyzed. These patterns correlate precisely with the HLA-D phenotype of the HCL donor as determined by reactivity in mixed lymphocyte culture. All DR4 HCL that belong to one of the well-defined HLA-D antigen groups (Dw4, DwlO, LD "40", LD "DYI", LD "KT2", or LD "TAS") have identical DR (-chain . Comprehensive populations studies with DR4-associated HTC have led to the description of several distinct DR4-associated HLA-D antigens, including Dw4, DwlO, LD "40", LD "DYT", and LD "KT2" (4-8). There are several possible explanations for these apparent DR4 "splits." First, HLA-Dw4 and DR4 may be the products of different loci, each coding for distinct "D region"-associated antigens. A second possibility is that Dw4 and DR4 are products of a single gene, the primary product of which is recognized by DR4 alloantisera, but through post-translational modification sufficient phenotype variation occurs to cause the differences detected in MLC by T cells. In a third model, the haplotypes expressing Dw4, DwlO, LD 40, LD DYT, and LD KT2 may be encoded by different alleles but share a common crossreactive or "supertypic" determinant recognized by DR4 alloantisera.To answer such questions, we have begun a detailed analysis of the two-dimensional gel electrophoresis patterns of DR antigens expressed by B-lymphoblastoid cell lines (HCL) derived from HLA-DR4 homozygous donors. Since most of the polymorphism attributed to DR molecules has been correlated with structural heterogeneity of the DR (&chain (9-13), we have prepared cell lines from donors of HLA-D homozygous typing cells representing each of the distinct HLA-D antigens associated with DR4 and have studied the relationships among isolated DR4 ,3-chain polypeptides. Our results demonstrate a striking correlation between the functionally distinct HLA-D phenotypes of individual HCL and the two-dimensional gel patterns of the DR ,B chains isolated from these HCL.MATERIALS AND METHODS HCL. Seventeen B-lymphoblastoid cell lines homozygous for an HLA-DR4-associated D locus were used in this study (Table 1). The specificities Dw4, Dw10, LD 40, LD DYT, LD KT2, and DB3 have been defined by the Eighth Workshop (5), by Reinsmoen and Bach (4), and by Nose et al. (2). In addition, we have used HCL derived from a unique HTC, "TAS," described by Amar et al. (20), that is mutually reactive in MLC with cells from each of the other clusters and that may represent yet another apparent split of the DR4 haplotype.Established B-lymphoblastoid cell lines were available for some of the above HTC, otherwise new B-lymphoblastoid lines were prepared from cryopreserved HTC by cocultivation with Epstein-Barr virus (EBV) as described (21). Briefly, 1-2 X 106

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B-Lymphoid cell lines derived fromHLA-D homozygous donors

Cited by 69 publications

References 19 publications

Specific HLA-DR4-associated histocompatibility molecules characterize patients with seropositive juvenile rheumatoid arthritis.

Specific HLA-DR4-associated histocompatibility molecules characterize patients with seropositive juvenile rheumatoid arthritis.

Definition of a possible genetic basis for susceptibility to acute myelogenous leukemia associated with the presence of a polymorphic Ia epitope.

Electrophoretic analysis of human HLA-DR antigens from HLA-DR4 homozygous cell lines: correlation between beta-chain diversity and HLA-D.

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