<b>Ion trap mass spectrometry of surfactins produced by <i>Bacillus subtilis</i> SZMC 6179J reveals novel fragmentation features of cyclic lipopeptides</b>

Bóka, Bettina; László, Manczinger; Kecskeméti, Anita; Chandrasekaran, M.; Kadaikunnan, Shine; Alharbi, Naiyf S.; Vágvölgyi, Csaba; Szekeres, András

doi:10.1002/rcm.7592

Cited by 25 publications

(51 citation statements)

References 49 publications

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“…When the wrong sfp was changed for a correct copy from a surfactin-producer strain of B. subtilis , both surfactin and fengycin production were intensively expressed in the transgenic line of strain 168 (Coutte et al 2010). Our study revealed that a single base deletion in the sfp gene enables strain SZMC 6179J to produce active phosphopantetheine transferase and allows the production of the lipopeptides fengycin and surfactin, which was previously proved by TLC and HPLC investigations (Bóka et al 2016; Vágvölgyi et al 2013). This single base deletion in the sfp gene also makes strain SZMC 6179J a potential biocontrol candidate with good antifungal properties (Table 4).…”

Section: Discussionsupporting

confidence: 67%

“…The depsipeptide gene clusters enabling the production of surfactins and fengycins are present in the genome of B. subtilis SZMC 6179J. Although the presence of a given gene cluster in the genome does not necessarily mean the synthesis of the corresponding antibiotic, the effective production of two antibiotics very important for antimicrobial effectiveness, the antibacterial surfactin and the antifungal fengycin, were proved earlier in the case of strain SZMC 6179J (Bóka et al 2016; Manczinger et al 2011; Vágvölgyi et al 2013). The explored SNPs and DIPs do not disturb the efficient expression of these gene clusters, as the fengycins and surfactins are produced by the strain.…”

Section: Discussionmentioning

confidence: 99%

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Genome analysis of a Bacillus subtilis strain reveals genetic mutations determining biocontrol properties

Bóka

László

Kocsubé

et al. 2019

World J Microbiol Biotechnol

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Several Bacillus strains are used as biocontrol agents, as they frequently have strong antagonistic effects against microbial plant pathogens. Bacillus strain SZMC 6179J, isolated from tomato rhizosphere, was previously shown to have excellent in vitro antagonistic properties against the most important fungal pathogens of tomato (Alternaria solani, Botrytis cinerea, Phytophthora infestans and Sclerotinia sclerotiorum) as well as several Fusarium species. Taxonomic investigations revealed that it is a member of the B. subtilis subsp. subtilis group and very closely related with the reference type strain B. subtilis subsp. subtilis 168. The sequenced genome of strain SZMC 6179J contains the genes responsible for the synthesis of the extracellular antibiotics surfactin, fengycin and bacilysin. Compared to strain 168, a prophage-like region is missing from the genome of SZMC 6179J, while there are 106 single nucleotide polymorphisms and 23 deletion-insertion polymorphisms. The high biocontrol potential of strain SZMC 6179J may results from a single base deletion in the sfp gene encoding the transcription factor of the surfactin and fengycin operons. Hypermutated regions reflecting short-time evolutionary processes could be detected in SZMC 6179J. The deletion-insertion polymorphism in the sfp gene and the detected hypermutations can be suggested as genetic determinants of biocontrol features in B. subtilis.Graphical Abstract Electronic supplementary materialThe online version of this article (10.1007/s11274-019-2625-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Genome analysis of a Bacillus subtilis strain reveals genetic mutations determining biocontrol properties

Bóka

László

Kocsubé

et al. 2019

World J Microbiol Biotechnol

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“…Then the supernatants were sedimented for 6 h at ambient temperature. Sedimentation was collected after centrifuge at 8000 × g for 10 min, then dissolved by methyl alcohol and the pH was adjusted to 7.0 (Lee et al, 2008; Bóka et al, 2016). The mixture was then stirred by magnetic stirrer at ambient temperature for 5 h. Sedimentation was removed after centrifuge at 8000 × g for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…Collision-induced dissociation (CID) analysis has been applied to authenticate structures of lipopeptides homolog. By far, there are no reports of dipolymer or tripolymer lipopeptides produced by Bacillus (Li et al, 2008; Tosco et al, 2015; Bóka et al, 2016; Urajová et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments

et al. 2017

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Antibiotic resistance has become one of the world’s most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M+H]+ m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was Rn-Glu1-Leu/Ile2-Leu3-Val4-Asp5-Leu6-Leu/Ile7. The lipopeptides showed well resistance to UV light and the change of pH and temperature.

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“…The production of lipopeptide by Bacillus species is based on the strains: some strains can coproduce two or three classes of lipopeptides, while others can yield only one class [11][12][13]. B. licheniformis MB01 produces surfactin only [12], B. subtilis K1 coproduces surfactins and iturins, while B.…”

Section: Velezensismentioning

confidence: 99%

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

Chen

Wang²,

Liu

et al. 2020

Preprint

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Background: There is an urgent need to discover alternative Bacillus biocontrol agents to control bacterial wilt. This study reports on a new lipopeptide-producing biocontrol strain FJAT-46737 and explores its lipopeptidic compounds, and this study investigates the antagonistic effects of these compounds.Results: Based on a whole genome sequence analysis, the new strain FJAT-46737 was identified as Bacillus velezensis, and seven gene clusters responsible for the synthesis of bioactive secondary metabolites in FJAT-46737 were predicted. The antimicrobial results demonstrated that FJAT-46737 exhibited broad-spectrum antimicrobial activities in vitro against three bacteria and three fungi. Pot experiments showed that the control efficiencies for tomato bacterial wilt of the whole cultures, the 2-fold diluted supernatants and the crude lipopeptide of FJAT-46737 were 66.2%, 82.0%, and 96.2%, respectively. The above results suggested that one of the antagonistic mechanisms of FJAT-46737 was the secretion of lipopeptides consisting of iturins, fengycins and surfactins. The crude lipopeptides had significant antagonistic activities against several pathogens (including Ralstonia solanacearum, Escherichia coli and Fusarium oxysporum) and fengycins were the major antibacterial components of the lipopeptides against R. solanacearum in vitro. Furthermore, the rich organic nitrogen sources (especially yeast extracts) in the media promoted the production of fengycin and surfactin by FJAT-46737. The secretion of these two lipopeptides was related to temperature fluctuations, with the fengycin content decreasing by 96.6% and the surfactins content increasing by 59.9% from 20 oC to 40 oC. The optimal temperature for lipopeptide production by FJAT-46737 varied between 20 oC and 25oC. Conclusions: The B. velezensis strain FJAT-46737 and its secreted lipopeptides could be used as new sources of potential biocontrol agents against several plant pathogens, and especially the bacterial wilt pathogen R. solanacearum.

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**Ion trap mass spectrometry of surfactins produced by Bacillus subtilis SZMC 6179J reveals novel fragmentation features of cyclic lipopeptides**

Cited by 25 publications

References 49 publications

Genome analysis of a Bacillus subtilis strain reveals genetic mutations determining biocontrol properties

Genome analysis of a Bacillus subtilis strain reveals genetic mutations determining biocontrol properties

Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments

Biocontrol of tomato bacterial wilt by the new strain Bacillus velezensis FJAT-46737 and its lipopeptides

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