Immunomodulatory Effects of Cyperus rotundus Extract on 7,12 Dimethylbenz[a]anthracene (DMBA) Exposed BALB/c Mice

Abstract

Background: The carcinogenic substance 7,12-Dimethylbenz[a]anthracene (DMBA) was commonly used to induce tumor formation in rodents. The development of tumor may trigger higher expression of pro-inflammatory cytokines, which in turn supports tumor progression. In this study, we examined the efficacy of Cyperus rotundus extract (CRE) that was reported to have anti-inflammatory properties. We focused on investigating the levels of activated T lymphocytes and the pro-inflammatory cytokines expressed by macrophages. Methods: Female BALB/c were injected with DMBA subcutaneously. The DMBA exposed mice were given CRE orally in three different doses; 63.33, 158.4, and 316.8 mg/kg. After 14 days, the levels of activated T lymphocytes and pro-inflammatory cytokines were analyzed using flow cytometry. Graphical analysis was done with FlowJo v10 and followed by statistical analysis. Results: The treatment of CRE reduced the population of CD4 and CD8 T cells. The number of activated CD4 and CD8 T cells were also significantly suppressed. The population of macrophages marked by CD11b cells was significantly reduced. Finally, the CRE treatment suppressed the levels of TNF-α, IFN-γ, IL-1β, and IL-6 expressed by macrophages. Conclusion: Our findings suggest that CRE could be a potential agent useful in therapeutic approaches for curing the disease caused by aberrant cells.

Effect of ethanolic extract of Cyperus rotundus reduces the surgical induced secondary lymphedema and oxidative stress in adult mice tail

“…Molecule’s present in CR has been reported to inhibit the pro-inflammation via the inhibition of the NF-κB and STAT3 pathways and ROS. (20)(21). As in the condition of SL, some of the studies have shown the expression of the pro-inflammatory gene which are been upregulated in animal models and patients with lymphedema.…”

Frankincense oil-loaded nanoemulsion formulation of paclitaxel and erucin: A synergistic combination for ameliorating drug resistance in breast cancer: In vitro and in vivo study

“… Gyamfi et al, 2018 and Sheng et al, 2018 reported that the pro-inflammatory cytokine IL-6 has been directly linked to breast cancer progression and risk. DMBA toxicity is known to trigger the release of inflammatory cytokines such as TNF-α, and IL-6 in breast cancer ( Ramadhani et al, 2020 ). EPNE significantly downregulated the level of inflammatory markers such as TNF-α, and IL-6 as compared to groups of the combination of ER + PTX, ER, and PTX alone in DMBA induced breast cancer group.…”

Immunomodulator Effect of Polyherbal Extract Turmeric-Meniran on Macrophages Profile of Mice Cancer Model by DMBA Injection

“…The most reliable and commonly used animal model of carcinogenesis to evaluate the therapeutic potential of a cancer drug candidate is the chemically injected animal model. 7,12-Dimethylbenz[a]anthracene (DMBA) is one of the most frequently used carcinogenic chemicals to model cancer in animals [19]. Furthermore, DMBA injection on mice causes immunosuppressants, such as reduced macrophage profile 1 (M1) cytokine levels [1].…”