In situbiosensing of the nanomechanical property and electrochemical spectroscopy ofStreptococcus mutans-containing biofilms

Liu, Bernard Haochih; Li, Kun Lin; Kang, Kai; Huang, Wen Ke; Liao, Jiunn Der

doi:10.1088/0022-3727/46/27/275401

Cited by 14 publications

(14 citation statements)

References 37 publications

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“…Consistent with earlier AFM (78) and electron microscopy (75,81,82) studies, the cells were elongated, had a smooth surface, and exhibited a well defined septum (arrow 1). In addition, annular structures were clearly visible at a certain distance from the poles (arrow 2).…”

Section: Volume 290 • Number 14 • April 3 2015supporting

confidence: 88%

Identification of a Supramolecular Functional Architecture of Streptococcus mutans Adhesin P1 on the Bacterial Cell Surface

Heim

Sullan

Crowley

et al. 2015

Journal of Biological Chemistry

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Background: P1 is an adhesin on the surface of Streptococcus mutans. Results: Adhesive P1 on the surface of S. mutans exhibits a macromolecular ultrastructure. Conclusion:The architecture of P1 on the surface of S. mutans plays a critical role in adherence. Significance: Recognizing the macromolecular assembly of P1 on the surface of S. mutans is critical to understanding the adhesive function of the molecule.

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Section: Volume 290 • Number 14 • April 3 2015supporting

confidence: 88%

Identification of a Supramolecular Functional Architecture of Streptococcus mutans Adhesin P1 on the Bacterial Cell Surface

Heim

Sullan

Crowley

et al. 2015

Journal of Biological Chemistry

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“…Our previous study on Streptococcus mutans showed the mechanical evolution on bacterial surface for 2 h, monitored by continuous AFM mechanical mapping, and the microbial samples were verified to remain alive within such duration [ 8 – 10 ]. To ensure the viability of the E. coli samples used in this work, the pre-studies on the bacterial samples were conducted, and the continuous change in surface adhesion for 4 h implied the cells remains alive for at least 4 h after sample preparation (shown in Additional file 1 ).…”

Section: Methodsmentioning

confidence: 99%

Identification of Characteristic Macromolecules of Escherichia coli Genotypes by Atomic Force Microscope Nanoscale Mechanical Mapping

Chang

Liu

2018

Nanoscale Res Lett

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The categorization of microbial strains is conventionally based on the molecular method, and seldom are the morphological characteristics in the bacterial strains studied. In this research, we revealed the macromolecular structures of the bacterial surface via AFM mechanical mapping, whose resolution was not only determined by the nanoscale tip size but also the mechanical properties of the specimen. This technique enabled the nanoscale study of membranous structures of microbial strains with simple specimen preparation and flexible working environments, which overcame the multiple restrictions in electron microscopy and label-enable biochemical analytical methods. The characteristic macromolecules located among cellular surface were considered as surface layer proteins and were found to be specific to the Escherichia coli genotypes, from which the averaged molecular sizes were characterized with diameters ranging from 38 to 66 nm, and the molecular shapes were kidney-like or round. In conclusion, the surface macromolecular structures have unique characteristics that link to the E. coli genotype, which suggests that the genomic effects on cellular morphologies can be rapidly identified using AFM mechanical mapping. Graphical AbstractQuantification of surface macromolecules of E. coli cells using AFM mechanical mapping. Surface macromolecules of cellular surface of three E. coli genotypes, MG1655, CFT073, and RS218, were characterized with the sizes ranging from 38 to 66 nm and with round or kidney-like shapes. The topography images were colored with adhesion mapping with the scale bars = 200 nm. Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2452-2) contains supplementary material, which is available to authorized users.

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“…The method was used for the investigation of properties of oxide layers, ceramics, nanocomposites, and solid electrolytes . Usability of NIM was confirmed also in branches far from materials engineering such as microbiology …”

Section: Introductionmentioning

confidence: 97%

Application of multisine nanoscale impedance microscopy to heterogeneous alloy surface investigations

Zieliński

Darowicki

2015

Surface & Interface Analysis

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In the recent years atomic force microscopy is recognized as valuable tool for investigation of surficial features of construction materials. It concerns, among other things, studies of changes caused by such phenomena as galvanic corrosion, passivation associated with the growth of oxide layers, or sensitization of austenitic steels with the formation of carbide phases. In addition, atomic force microscopy allows easy coupling with impedance technique which is especially promising because of its versatility in investigations of both conductive and dielectric materials. The authors discuss the possibility of application of such type of measurement, known as impedance microscopy.

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In situbiosensing of the nanomechanical property and electrochemical spectroscopy ofStreptococcus mutans-containing biofilms

Cited by 14 publications

References 37 publications

Identification of a Supramolecular Functional Architecture of Streptococcus mutans Adhesin P1 on the Bacterial Cell Surface

Identification of a Supramolecular Functional Architecture of Streptococcus mutans Adhesin P1 on the Bacterial Cell Surface

Identification of Characteristic Macromolecules of Escherichia coli Genotypes by Atomic Force Microscope Nanoscale Mechanical Mapping

Application of multisine nanoscale impedance microscopy to heterogeneous alloy surface investigations

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