<b>Glycoproteins: a Tribute to Albert Neuberger</b> A Joint Colloquium of the Carbohydrate Groups of the Biochemical Society and the Chemical Society

Ak, Allen; Tn, Palmer

doi:10.1042/bst0070781

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Better results were obtained with an affinity adsorbent prepared by amidating CH-Sepharose with D-galactosamine [5]. At pH 8, even in 2 M NaCl, the lectin is adsorbed completely, whereas at pH 5 about two-thirds leaves the column, the residual activity is eluted on raising the NaCl concentration to 2 M. 1 ml of the affinity gel retains more than 3 mg of the purified lectin.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Purification and Properties of Blood‐Group‐Specific Lectins from Vicia cracca

Rüdiger

1977

European Journal of Biochemistry

View full text Add to dashboard Cite

The lectins from the seeds of Vicia cracca react specifically with human blood group A erythrocytes. They were purified by affinity chromatography on an adsorbent containing matrix‐bound N‐acyl‐d‐galactosamine. By a continuous pH gradient the lectins could be separated into two fractions each of which was shown to consist of several agglutinating species. The behaviour of both fractions in affinity chromatography was paralleled by the pH dependence of the interaction with the hapten sugar N‐acetyl‐d‐galactosamine. Both lectin fractions have the same molecular (125000) and subunit (33000) weights, display the same pH dependence of their titre against A1 erythrocytes, and bind to N‐acetyl‐d‐galactosamine at pH 8 with the same constant of about 6 × 103M−1.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Affinity adsorbents were prepared either by coupling p-aminophen yl N-acetyl-fi-D-galactosdminide to cyanogen-bromide-activated Sepharose 4 B [4J or by amidation of CH-Sepharose with an amino sugar using the water-soluble l-ethyl-3-(3-dimethylamino-propy1)-carbodiimide as a condensing agent [5].…”

Section: Methodsmentioning

confidence: 99%

Purification and Properties of Blood‐Group‐Specific Lectins from Vicia cracca

Rüdiger

1977

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

Glycoproteins: a Tribute to Albert Neuberger A Joint Colloquium of the Carbohydrate Groups of the Biochemical Society and the Chemical Society

Cited by 1 publication

References 0 publications

Purification and Properties of Blood‐Group‐Specific Lectins from Vicia cracca

Purification and Properties of Blood‐Group‐Specific Lectins from Vicia cracca

Contact Info

Product

Resources

About