B cell tolerance: Putting the horse before the cart

Kelsoe, Garnett

doi:10.1002/art.30162

Arthritis & Rheumatism

2011

DOI: 10.1002/art.30162

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B cell tolerance: Putting the horse before the cart

Garnett Kelsoe¹

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Cited by 3 publications

(2 citation statements)

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“…The mapping and structural features of B‐cell epitopes and apotopes (determinants expressed on the surface of apoptotic cells) on Ro60, Ro52 and La and the function of these autoantigens have been reviewed elsewhere 10 , 11 , 12 , 13 . Here, we present recent findings on the pathologic effects of these autoantibodies and an immunoproteomic approach to analyze the secreted Ro/La autoantibody repertoire.…”

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confidence: 98%

Pathogenicity and proteomic signatures of autoantibodies to Ro and La

et al. 2012

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Ro/SSA and La/SSB comprise a linked set of autoantigens that are clinically important members of the extractable nuclear antigen family and key translational biomarkers for lupus and primary Sjö gren's syndrome. Autoantibodies directed against the Ro60 and La polypeptide components of the Ro/La ribonucleoprotein complex, and the structurally unrelated Ro52 protein, mediate tissue damage in the neonatal lupus syndrome, a model of passively acquired autoimmunity in humans in which the most serious manifestation is congenital heart block (CHB). Recent studies have concentrated on two distinct pathogenic mechanisms by which maternal anti-Ro/La autoantibodies can cause CHB: by forming immune complexes with apoptotic cells in developing fetal heart; and/or by acting as functional autoantibodies that cross-react with and inhibit calcium channels. Although the precise role of the individual autoantibodies is yet to be settled, maternal anti-Ro60 and anti-Ro52 remain the most likely culprits. This article will discuss the molecular pathways that culminate in the development of CHB, including the recent discovery of b2 glycoprotein I as a protective factor, and present a proteomic approach based on direct mass spectrometric sequencing, which may give a more representative snapshot of the idiotype repertoire of these autoantibodies than genomic-based technologies.

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confidence: 98%

Pathogenicity and proteomic signatures of autoantibodies to Ro and La

et al. 2012

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“…The etiology and pathogenesis of IBD have been associated with many factors, including the interaction of environmental, genetic and immune factors, which can initiate an abnormal immune response and chronic inflammation 2 . Therefore, a cure for IBD would block inflammation and also re-establish immunological tolerance 3 . The current standard drug regimen focuses on suppression of inflammation using 5-aminosalicylic acid, corticosteroids and immunomodulators 4 5 .…”

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Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells

Liu

Ren

Wang

et al. 2015

Sci Rep

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The adoptive transfer of the natural regulatory B cells and macrophages should be a useful treatment for inflammation and autoimmune disease. However, it is usually difficult to isolate these cells from the tissues and expand them. Here, we investigated the feasibility of adoptively transferring peritoneal cells (PCs) as a treatment for DSS-induced colitis. We found that peritoneal cavity can provide an easily accessible site for harvesting enough number of PCs, namely, two-dose PCs for the treatment from a mouse in one operation. Adoptive therapy of these cells from healthy mice or those with disease is effectively in reducing the disease activity score. The natural B cells and macrophages of the infused PCs can selectively migrate to lesion sites and regulate the expression of Stat3, NF−κB, Smad3 and Smad7. Additionally, PCs exert dual activity of IL-10 and TGF-β secreted spontaneously by both peritoneal B cells and macrophages, which in turn enhance the induction of regulatory B cells and Macrophages in microenvironment of inflammation. Moreover, PCs can re-establish immunological tolerance in the OVA-immunized mice. Thus, our findings provide a new strategy for colitis therapy and could be of importance in additional exploration of other inflammation and autoimmune diseases therapy.

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Comparison of assays for the detection of West Nile virus antibodies in equine serum after natural infection or vaccination

Joó

Bakonyi

Szenci

et al. 2017

Veterinary Immunology and Immunopathology

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2West Nile virus (WNV) mainly infects birds, horses and humans. Outcomes of the infection range from light uncharacteristic signs to fatal neurologic disease. The main objectives of the present study were to measure serum IgG and IgM antibodies in naturally exposed and vaccinated horses and to compare results of hemagglutination-inhibition test (HIT), enzymelinked immunosorbent assay (ELISA) and plaque reduction neutralization test (PRNT).Altogether 224 animals were tested with HIT for WNV antibodies and 41 horses were simultaneously examined with ELISA and PRNT. After primary screening for WNV antibodies, horses were vaccinated. Samples were taken immediately before and 3-5 weeks after each vaccination. McNemar chi-squared and percent agreement tests were used to detect concordance between HIT, ELISA and PRNT.Analyses by HIT confirmed the presence of WNV antibodies in 27/105 (25.71 %) from naturally exposed horses. Sera from 57/66 (86.36%) vaccinated animals were positive before the first booster and from 11/11 (100%) before the second booster. HIT was less sensitive when detecting IgG antibodies. We could detect post vaccination IgM in 13 cases with MAC-ELISA and in 7 cases with HIT.WNV is endemic in Hungary causing regular natural infections. Protective antibodies could not be measured in each individual case 12 months after primary injections; protection is more reliable after the first yearly booster. Based on our findings it was not be possible to differentiate infected horses from recently vaccinated horses using IgM antibody capture ELISA (MAC-ELISA). HIT does not substitute ELISA or PRNT when detecting IgG, but was useful tool in this study to gain statistical information about the tendencies within a fixed population of horses.

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B cell tolerance: Putting the horse before the cart

Cited by 3 publications

References 39 publications

Pathogenicity and proteomic signatures of autoantibodies to Ro and La

Pathogenicity and proteomic signatures of autoantibodies to Ro and La

Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells

Comparison of assays for the detection of West Nile virus antibodies in equine serum after natural infection or vaccination

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