B-cell specific Moloney leukemia virus insert site 1 and peptidyl arginine deiminase IV positively regulate carcinogenesis and progression of esophageal squamous cell carcinoma

Abstract

Abstract. High expression of B-cell specific Moloney leukemia virus insert site 1 (Bmi-1) and peptidyl arginine deiminase IV (PADI4) is associated with esophageal carcinoma. However, few studies have investigated the association between the Bmi-1 and PADI4 genes. The aim of the present study was to evaluate the expression of Bmi-1 and PADI4 and identify the association between the Bmi-1 and PADI4 genes in esophageal squamous cell carcinoma (ESCC) tissues. Bmi-1 and PADI4 gene expression levels were measured using immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction in ESCC tissues from 86 patients who had not received pre-operative chemoradiation. The results revealed that the Bmi-1 and PADI4 genes had increased expression in carcinoma tissues compared with pericarcinous tissue (P<0.05). Bmi-1 gene expression was revealed to be associated with differentiation degree, clinical stage and lymph node metastasis (P<0.05), but had no association with gender, age or depth of invasion (P>0.05). The expression of PADI4 was associated with clinical stage, depth of invasion and lymph node metastasis (P<0.05), but was not associated with gender, age or differentiation degree (P>0.05). In addition, there was a positive association between Bmi-1 and PADI4 gene expression in ESCC (P<0.05). These results indicated that Bmi-1 and PADI4 positively regulate carcinogenesis and progression of ESCC.

Peptidyl Arginine Deiminases in Chronic Diseases: A Focus on Rheumatoid Arthritis and Interstitial Lung Disease

“…It has been found that tumors, including squamous cell esophageal carcinoma, have an abnormally increased expression in PADI4. Furthermore, this increase has been associated with carcinogenesis, progress, and metastasis in other tumors [44]. However, expression decreases when the tumor is extirpated [45].…”

Role of the PADI family in inflammatory autoimmune diseases and cancers: A systematic review

“…In esophageal squamous cell carcinoma (ESCC), PADI4 can stimulate the growth of ESCC cells and upregulate CA9 to promote ESCC metastasis ( 171 ). PADI4 synergizes with B-cell-specific Moloney leukemia virus insertion site 1 (Bmi-1) to promote the carcinogenesis and progression of ESCC ( 172 ). In prolactinomas and growing prolactinomas, PADI2 and PADI4 can promote the proliferation of cancer cells by catalyzing the citrullination of histones and upregulating the oncogenes HMGA1, N-MYC and IGF-1 ( 149 ).…”