Azole Resistance in Aspergillus fumigatus: A Consequence of Antifungal Use in Agriculture?

Berger, Sarah; Chazli, Yassine El; Babu, Ambrin Farizah; Coste, Alix T.

doi:10.3389/fmicb.2017.01024

Cited by 188 publications

(155 citation statements)

References 20 publications

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“…Our findings, and that of the Welsh study (13), appear to contradict the hypothesis that UK AR Af is driven by the environmental application of azoles in arable agriculture (35, 36). Indeed, of the 53 samples collected directly on or surrounding agricultural land, zero azole-tolerant isolates were identified.…”

Section: Discussioncontrasting

confidence: 99%

Elevated prevalence of azole resistant Aspergillus fumigatus in urban versus rural environments in the United Kingdom

Sewell¹,

Zhang²,

Brackin³

et al. 2019

Preprint

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8Azole resistance in the opportunistic pathogen Aspergillus fumigatus is increasing, dominated primarily 9 by two environmentally-associated resistance alleles: TR34/L98H and TR46/Y121F/T289A. Using an 10 environmental sampling strategy across the South of England we assess the prevalence of azole resistant 11 A. fumigatus (ARAf) in soil samples collected in both urban and rural locations. We characterise the 12 susceptibility profiles of the resistant isolates to three medical azoles, identify the underlying genetic 13 basis of resistance and investigate their genetic relationships. ARAf was detected in 6.7% of the soil 14 samples, with a higher prevalence in urban (13.8%) compared to rural (1.1%) locations. Nineteen 15 isolates were confirmed to exhibit clinical breakpoints for resistance to at least one of three medical 16 azoles, with 18 isolates exhibiting resistance to itraconazole, four to voriconazole, with two also showing 17 additional elevated minimum inhibitory concentration to posaconazole. Thirteen of the resistant isolates 18 harboured the TR34/L98H resistance allele and six isolates carried TR46/Y121F/T289A allele. The 19 azole-19 resistant isolates were spread across five csp1 genetic subtypes, t01, t02, t04B, t09 and t18 with t02 the 20 predominant subtype. Our study demonstrates that ARAf can be easily isolated in the South of England, 21 especially in urban city centres, which appear to play an important role in the epidemiology of 22 environmentally-linked drug resistant A. fumigatus. 23 24

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Section: Discussioncontrasting

confidence: 99%

Elevated prevalence of azole resistant Aspergillus fumigatus in urban versus rural environments in the United Kingdom

Sewell¹,

Zhang²,

Brackin³

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…The azoles used in agriculture and in clinical settings target the same active site, which means that pathogenic fungi can engage shared modes of resistance [70]. Faria-Ramos et al [71] and Berger et al [72] reported the resistance of Aspergillus sp. to clinically relevant azole fungicides was due to exposure to agricultural azoles which led to the emergence of cross-resistance.…”

Section: Discussionmentioning

confidence: 99%

Three-Locus Sequence Identification and Differential Tebuconazole Sensitivity Suggest Novel Fusarium equiseti Haplotype from Trinidad

Villafana

Rampersad

2020

Pathogens

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The Fusarium incarnatum-equiseti species complex (FIESC) consists of 33 phylogenetic species according to multi-locus sequence typing (MLST) and Genealogical Concordance Phylogenetic Species Recognition (GCPSR). A multi-locus dataset consisting of nucleotide sequences of the translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1), and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish among phylogenetic species within the FIESC isolates infecting bell pepper in Trinidad. Three phylogenetic species belonged to the Incarnatum clade , and one species belonged to the Equiseti clade . Specific MLST types were sensitive to 10 µg/mL of tebuconazole fungicide as a discriminatory dose. The EC50 values were significantly different among the four MLST groups, which were separated into two homogeneous groups: FIESC-26a and FIESC-14a, demonstrating the "sensitive" azole phenotype and FIESC-15a and FIESC-16a as the "less sensitive" azole phenotype. CYP51C sequences of the Trinidad isolates, although under positive selection, were without any signatures of recombination, were highly conserved, and were not correlated with these azole phenotypes. CYP51C sequences were unable to resolve the FIESC isolates as phylogenetic inference indicated polytomic branching for these sequences. This data is important to different research communities, including those studying Fusarium phytopathology, mycotoxins, and public health impacts.

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“…Since azole‐resistant strains were first identified in 1997, the rate of resistance has risen rapidly to account for ∼5–6% of A. fumigatus isolates . There is evidence to suggest that mounting rates of azole resistance are linked to the exposure of A. fumigatus to azole‐based agricultural fungicides in the environment and the resultant selection for resistant strains …”

Section: Emerging Threats Of Drug‐resistant Filamentous Fungal Pathogensmentioning

confidence: 99%

“…134,135 There is evidence to suggest that mounting rates of azole resistance are linked to the exposure of A. fumigatus to azole-based agricultural fungicides in the environment and the resultant selection for resistant strains. [136][137][138][139] Given the clinical relevance of Aspergillus spp., the emergence of opportunistic A. terreus, the third leading cause of global IA, is of particular concern. For example, A. terreus presents a growing problem among patients with hematological malignancies (HMs), 140,141 as it causes severe infections and has a propensity to disseminate, resulting in systemic patient infections.…”

Section: Emerging Threats Of Drug-resistant Filamentous Fungal Pathogensmentioning

confidence: 99%

New pathogens, new tricks: emerging, drug‐resistant fungal pathogens and future prospects for antifungal therapeutics

Geddes‐McAlister

Shapiro

2018

Annals of the New York Academy of Sciences

131

103

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Fungal pathogens are a growing threat to public health. As human immunodeficiency becomes increasingly common, fungal infections are becoming more prevalent. The use of antifungal agents for prophylaxis and treatment of fungal infections has favored the emergence of previously rare or unidentified species of drug-resistant fungal pathogens, including several Candida and Cryptococcus species, as well as mold pathogens. As these new and increasingly drug-resistant fungal pathogens continue to emerge, novel strategies for rapid identification and treatment are necessary to combat these life-threatening infections.

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Azole Resistance in Aspergillus fumigatus: A Consequence of Antifungal Use in Agriculture?

Cited by 188 publications

References 20 publications

Elevated prevalence of azole resistant Aspergillus fumigatus in urban versus rural environments in the United Kingdom

Elevated prevalence of azole resistant Aspergillus fumigatus in urban versus rural environments in the United Kingdom

Three-Locus Sequence Identification and Differential Tebuconazole Sensitivity Suggest Novel Fusarium equiseti Haplotype from Trinidad

New pathogens, new tricks: emerging, drug‐resistant fungal pathogens and future prospects for antifungal therapeutics

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